THERMO SCIENTIFIC Electroporation Cellject

The Nucleofector technology is a major improvement of the well known electroporation technology. The (more) Nucleofector technology involves a combination of optimized electrical parameters and specific Nucleofector solutions for different cell types. This combination permits the crucials step: delivery of the DNA of interest straight into the nucleus. As the DNA quickly reaches the nucleus, expression can begin without delay. The nucleofected cells are thus ready for analysis after a short incubation time of only 2 – 8 hours. DNA quality: 1 – 5 µg plasmid concentration per nuclefection sample (100 µl), plasmid concentration can be increased up to 10 µg per sample. This may lead to higher gene transfer efficiencies, but at the same time may increase mortality of the cells. Cell density: 2 x 105 – 1 x 107 cells per nuclefection sample for suspensions cells 2 x 105 – 2 x 106 cells per sample for adherent cells Cell densities of < 2 x 105 cells per smaple are not advisable beacause the gene transfer efficiency may decrease and mortality may increase. Dimensions (WxDxH): 300 x 230 x x110 mm Weight: 2.5 kg

The Gemini X2 Systems is designed for researchers who need the ultimate experiment flexibility. In o (more)ne easy setup, square wave and exponential decay waves can be applied to cells in any format. These systems incorporate remote operation functionality via footswitch or PC and internal log storage of experiment data for easy optimization, quality control requirements and troubleshooting. Electroporation of suspension cells can be achieved in 96-well plates with the HT plate handler. Usable with all cell electroporation Features: Square Waveform, Multi-Pulsing Square Wave, Exponential Decay Waveform, Multi-Pulsing Exponential Decay, Resistance/Pulse Monitoring, Experiment Log Storage, Preprogrammed Protocols, Unlimited Custom Protocol Storage, Remote Operation, PC Communications Voltage Range: 5 to 3000 V Pulse Width Range: 10 μs to 5 s Pulse Interval: 100 ms to 30 s Dimensions (DxWxH): 324 x 286 x 216 mm Weight: 7.3 kg

The offered instrument / system is fully functional tested and ready to use. FURTHER DETAILS TO FOLL (more)OW

The Gene Pulser Xcell System is a flexible, modular electroporation system for transfecting every ce (more)ll type from primary, suspension, and difficult-to-transfect cells, including T cells, to bacteria and fungi. The system is composed of a main unit, two accessory modules, the capacitance extender (CE module) and the pulse controller (PC module), and a ShockPod cuvette chamber. Features and Benefits Universal electroporation — transfects all cell types, from primary, stem, and T cells to bacteria and fungi Flexibility with your experiment parameters for the highest transfection efficiency and viability Preset protocols — ready-to-use preoptimized protocols for frequently used mammalian and bacterial cell types Manual operation — allows for entry and storage of new parameters or editing of all parameters Optimization protocols — enables the best conditions to be determined using incremental voltage steps Exponential and square waveforms — allows for optimal delivery Confidence in your results — uses PulseTrac circuitry and arc-protection features to ensure sample protection and reproducible results Protocol library — collection of electroporation protocols for every cell type including primary, immortal, and bacterial cells Prevalidated published protocols — saves time optimizing conditions Easily accessed data management — storage and recall of parameters and results from previous 100 experiments, useful for troubleshooting Output Waveform: Exponential or square Output Voltage: 10 – 3,000 V Capacitance: 10 – 500 V, 25 – 3,275 µF in 25 µF increments, 500 – 3,000 V, 10, 25, 50 µF Resistance (parallel): 50 – 1,000 Ω in 50 Ω increments, plus infinity Sample resistance: 20 Ω minimum at 10 – 2,500 V, 600 Ω minimum at 2,500 – 3,000 V Square-wave timing: 10 – 500 V: 0.05 – 10 ms in 0.05 ms increments, 10 – 100 ms pulse in 1 ms increments, 1 – 10 pulses, 0.1 – 10 sec interval/ 500 – 3,000 V: 0.05 – 5 ms in 0.05 ms increments, 1 – 2 pulses, 5 sec minimum interval Dimensions (WxDxH): 310 x 300 x 280 mm Weight: 11.6 kg

Digital display for speed and temperature AM4 Digital PRO is the ideal magnetic stirrer for precise  (more)thermoregulation. With this instrument it is possible to set the temperature up to 370°C and the stirring speed from 50 to 1500 rpm. SpeedServo ™ ensures a constant speed even when the viscosity changes (counter-reaction). In addition, the digital display allows you to precisely set the stirring speed and temperature. Excellent thermoregulation with Pt100 and VTF The AM4 Digital PRO package includes the Pt100 external probe, for thermoregulation up to 250°C, with an accuracy of ± 1.0°C. AM4 Digital PRO can be connected to VTF ensuring perfect thermoregulation up to 300°C, with an exceptional accuracy of ± 0.5°C. The exclusive CerAlTop ™, made of ceramic coated aluminum alloy, guarantees exceptional temperature homogeneity and excellent resistance and heat transmission. Maximum protection and comfort The AM4 Digital PRO hotplate stirrer guarantees a higher level of safety and protection for the user. The control panel is easily accessible, is positioned at a safe distance from heat sources and is protected from any damage caused by liquid leaks thanks to a special drain groove. AM4 Digital PRO can be programmed with a maximum safety temperature, useful for some particular applications that require not to exceed certain temperatures, and avoid accidental overheating. In addition, a 'Hot Plate Warning' is activated and remains displayed even when the instrument is turned off, until the temperature of the plate has cooled below 50°C. The die-cast structure is designed so that accidental spills of liquid cannot reach the internal parts of the hot plate agitator.

Polyscience SD15R-30-A11B 15L Refrigerated Circulator, Standard Digital (-30 to 170C), 120V, 60HzPol (more)yScience Circulating Bath with Standard DigitalTemperature Controller. Extremely easy to use and maintain, it combines designinnovation with highly intuitive operation to deliver convenient and reliableliquid temperature control for a wide range of applications.Features Large, universal icon and English display Swivel 180™ Rotating Controller On-board connectivity: RS-232 serial output On-screen prompts Automatic performance optimization Single-point calibration capability INCLUDED: The Polyscience SD15R-30-A11B 15L Refrigerated Circulator, -30°C to 170°C, 915W; 120V includes: - Power Cord - Operator's Guide - Manufacturer's Documentation - 2 Year Manufacturer's Warranty

Mettler Toledo 20KGF2 Class Calibration Weight This was removed from a laboratory where it was surpl (more)us to requirements. The unit is in good cosmetic condition but the case has marks and cracks from use. The top of the case is broken.

incubator DiaMed 37SI New New equipment (never used) Incubator fo 24 ID-Card 37°C ± 0.1°C External d (more)imensions: 31 x 19 x 34cm Internal dimensions (L) x (L): 20.5 x 20.5 cm Weight : 6kg

The offered instrument / system is fully functional tested and ready to use. FURTHER DETAILS TO FOLL (more)OW