THERMO SCIENTIFIC Electroporation Cellject

The Nucleofector technology is a major improvement of the well known electroporation technology. The (more) Nucleofector technology involves a combination of optimized electrical parameters and specific Nucleofector solutions for different cell types. This combination permits the crucials step: delivery of the DNA of interest straight into the nucleus. As the DNA quickly reaches the nucleus, expression can begin without delay. The nucleofected cells are thus ready for analysis after a short incubation time of only 2 – 8 hours. DNA quality: 1 – 5 µg plasmid concentration per nuclefection sample (100 µl), plasmid concentration can be increased up to 10 µg per sample. This may lead to higher gene transfer efficiencies, but at the same time may increase mortality of the cells. Cell density: 2 x 105 – 1 x 107 cells per nuclefection sample for suspensions cells 2 x 105 – 2 x 106 cells per sample for adherent cells Cell densities of < 2 x 105 cells per smaple are not advisable beacause the gene transfer efficiency may decrease and mortality may increase. Dimensions (WxDxH): 300 x 230 x x110 mm Weight: 2.5 kg

The BTX HT-200 plate handler allows for the Highthroughput System HT 25 or HT 96 for advanced molecu (more)lar delivery. The HT 96 (96-well) plates or the HT 25 (25-well) plates can be electroporated using a BTX generator (models: ECM 830 and ECM 630) with a plate handler. The Model HT-200 automatically switches columns in a Multi-well plate during electroporation using the Auto-track sensing featute. The Auto-track sensing allows rapid electroporation of an entire plate by setting up all columns and pressing one button. Voltage Range: 0 to 3000V DC Pulse Length Range: 10 µsec to 10 sec Pulse Number Range: 1 to 99 Dimensions (WxDxH): 230 x 215 x 140 mm Weight: 4.8 kg Including: 1x 96-Well Disposable Electroporation Plates, 4 mm, 250 µl, pkg. of 1 1x 96-Well Disposable Electroporation Plates, 2 mm, 125 µl, pkg. of 1 1x HT 25-Well Adapter for Plate Handler

The Gemini X2 Systems is designed for researchers who need the ultimate experiment flexibility. In o (more)ne easy setup, square wave and exponential decay waves can be applied to cells in any format. These systems incorporate remote operation functionality via footswitch or PC and internal log storage of experiment data for easy optimization, quality control requirements and troubleshooting. Electroporation of suspension cells can be achieved in 96-well plates with the HT plate handler. Usable with all cell electroporation Features: Square Waveform, Multi-Pulsing Square Wave, Exponential Decay Waveform, Multi-Pulsing Exponential Decay, Resistance/Pulse Monitoring, Experiment Log Storage, Preprogrammed Protocols, Unlimited Custom Protocol Storage, Remote Operation, PC Communications Voltage Range: 5 to 3000 V Pulse Width Range: 10 μs to 5 s Pulse Interval: 100 ms to 30 s Dimensions (DxWxH): 324 x 286 x 216 mm Weight: 7.3 kg

This BTX Electro Square Porator ECM 830 was removed from a university lab where it was surplus to re (more)quirements. The item powers on and is in reasonably good cosmetic condition, we are unable to test it further at our facility.

Electroporator Lonza 4D-Nucleofactor System. Formed by two modules: unit Core (control unit) and un (more)it-X unit-transfection). Unit X performs 16-to 32-transfections per minute. Includes instruction manual and original packaging. 2014. Specifications: 240 V; 50-60 Hz The Core Unit - Controlling the 4D-Nucleofactor™ System The X unit – Supporting Nucleofection™ of various cell numbers in different formats

Imaging performance Cell size: 5 – 300 microns (Cellometer CHT4-PD300 Slides are required for cells  (more)> 80 microns in diameter) Concentration range: 105 – 107 cells/ml Bright field imaging, fluorescent imaging and pattern-recognition software to quickly and accurately decluster, identify and count individual cells Fluorescent optics Excitation/Emission: 470nm/535nm Example fluorophores: Acridine Orange (+DNA) Calcein AM CFDA SYTO 9 SYTO 13 Excitation/Emission: 540nm/605nm Example fluorophores: Propidium iodide Ethidium bromide Dimensions (WxDxH): 280 x 300 x 330 mm Weight: 11.8 kg

The Model 400 Hybridization oven is a high precision rotating convection oven for Northern blot and  (more)Southern blot hybridizations and other applications.

Lab-Bubble Still Air PCR Enclosure - White - BUB-PCR-SA-WHT This was removed from a university lab w (more)here it was surplus to requirements. The unit is in good cosmetic condition with minor marks

Stereomicroscope trinocular Nexius Zoom NZ.1903-P. Objective-zoom 0.67 to 4.5 x, magnification of 6. (more)7 x to 45x, light incidente and transmitted 3-W LED. SPECIFICATIONS EYE Pair of wide-field eyepieces HWF10x/22 mm With adjustment of ± 5 diopters built-in. HEAD Trinocular head with tube holder eyepieces inclined 45°. Distance inter-pupillary adjustable between 54 and 75 mm It has a port trinocular splitter with a beam of light (50:50) OBJECTIVES Objective Zoom 1:6.7, with increases of 0.67 x to 4.5 x. Field of view from 33 mm to 4.9 mm working Distance 110 mm Lens lens optional 0.5 x, 0.75 x, 1.5 x and 2.0 x. All optical components are anti-rust and treatment of layers of anti-reflection for maximum light transmission. INCREASES Working distance and field of view with eyepieces supplied in the standard equipment HWF10x/22 mm D. T.* working Distance STAND Flatbed large format with column (model-P) bracket and movable in height with coarse by rack and pinion system mounted on the guides, ball bearings, high-precision, dual-LED lighting system 3W, base with 2 clamps-fastening of the sample, LIGHTING Incident and transmitted LED 3W power internal 100-240 Vac. Both illuminators can be used simultaneously, and enable the regulation of intensity independent. PACKAGING Supplied with power cable, protective cover, replacement fuse and user manual. Case of polystyrene.

Pallet scales is stable and proved 4 load cell construction designed for weighing pallets. The load  (more)to be weighed can be placed on the platform by a standard fork-lift truck. The pallet scales C315.4P series is manufactured in mild steel powder coated version. The H315/4P series features wheels and handles for easy transporting the scales to its place of use. The scales comes standard with an indicator PUE C315 series featuring backlit LCD display and function buttons. The indicator is installed on a long cable. The scale is powered from mains 230V AC, RS 232 interface comes standard. Scales is available in dual range version on surcharge.

Labconco RapidVap N2 48-Place Block for 16 mm tubesFor use withRapidVap N2/48 Evaporation System. Bl (more)ock tube capacity: 48. Sample tube size:16 x 150 mm OD tubes, up to 23 ml. Sample volume: 17 ml. Actual sample volumedepends on tube shape, height and vortex speed.Blocks fitinside RapidVap Evaporation Systems. Glassware is required (not included). INCLUDED: The Labconco 7482300 RapidVap N2 48-Place Block for 16 mm tubes includes: - Manufacturer's Documentation - 1 Year Manufacturer's Warranty