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Sartorius Stedim Biostat B Twin Benchtop Bioreactor System with 2 L Jacketed Autoclavable Vessels
Product Description
The Sartorius Stedim Biostat B Twin is a compact, benchtop bioreactor system designed for precise control of mammalian cell culture processes in biotechnology and molecular diagnostics. It features two autoclavable 2-liter double-wall (jacketed) glass vessels with a working volume range of 0.4–2 L and total volume of 3 L each, optimized for small-scale parallel bioprocess experiments and seed train development.
Key technical specifications include:
- Agitation System: Direct-drive 200 W motor with a low-shear 3-blade segment impeller, providing agitation speeds from 20 to 2000 rpm to maintain homogeneous culture conditions while minimizing cell damage.
- Temperature Control: Open thermostat system with recirculation pump and automatic cooling water valve, maintaining temperatures up to 80 °C and heating capacity of 600 W, ensuring reproducible thermal regulation essential for cell metabolism.
- Gas Management: Integrated four-gas mixing capability (Air, O₂, N₂, CO₂) with precision thermal mass flow controllers (±1% full scale) and sparger aeration system for controlled oxygenation and pH regulation, critical for maintaining optimal dissolved oxygen and gas composition in biopharmaceutical development.
- Peristaltic Pumps: Multiple Watson Marlow 114 pumps for medium addition, acid/base feed, and foam dosing, supporting automated feeding strategies and process regulation in mammalian cell culture workflows.
- Sensors and Control Interfaces: Dual sets of pH, dissolved oxygen (DO), and temperature probes with high accuracy (e.g., Pt100 temperature sensors ±0.1 °C), plus extensive digital I/O ports supporting substrate weight monitoring, foam sensors, and feedback loops for tight process control.
- Dimensions and Power: Compact footprint (35×82.2×43 cm), weighing 55 kg, suitable for academic and industrial biotechnology labs, operating on standard 120 V, 60 Hz power.
This bioreactor system is essential for cell culture process development, including CHO and HEK cell lines, media optimization, and small-scale bioprocess screening. It supports bioprocess optimization and scale-up applications widely used in biomanufacturing, synthetic biology research, and molecular diagnostics.
The Biostat B Twin allows for parallel culture experimentation with reliable sterility via autoclavable glass vessels, and is widely adopted for live-cell bioprocess monitoring, biotechnology development pipelines, and preparation of seed train cultures for larger production vessels. Its modular design accommodates customization with alternative vessel sizes, impellers, and external feed pumps, making it critical instrumentation in biotechnology process development environments.
Overall, the Biostat B Twin embodies a robust platform for controlled small-scale mammalian cell culture, integrating advanced agitation, temperature regulation, gas supply precision, and automated feed delivery — all crucial for reproducible bioprocessing in clinical diagnostics, molecular biology, and biopharmaceutical production.
Primary keywords: benchtop bioreactor, jacketed autoclavable vessel, mammalian cell culture bioreactor, gas mixing bioreactor, bioprocess control system
Long-tail keywords: small-scale parallel bioprocess experiments, essential for molecular cloning workflows, widely adopted in synthetic biology research, critical in biopharmaceutical production pipelines, integrated pH and dissolved oxygen monitoring, thermal mass flow controlled aeration, live-cell bioprocess monitoring system, biomanufacturing seed train preparation, bioreactor controller with multi-pump feeding strategy
Item Stats
Product details
| Stock | In Stock |
| Brand | Sartorius Stedim |
| Product Code | 65534 |
| Condition | Pre-Owned |
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