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Lasers:
Blue Solid State: 488 nm, 55 mW, Software Controlled
Red Solid State Diode: 638 nm, 50 mW, Software Controlled
Violet Solid State: 405 nm, 80 mW, Software Controlled
Configuration: Nominal 90 µm spatially separated beam spots
Flow cell: 430 µm x 180 µm rectangular channel
Collection optics: Fixed integrated optics and quartz flow cell design with a numerical aperture of > 1.2
Detector filters:
Forward Scatter: 488/20
Blue Laser: 525/40, 575/30, 614/20 , 675/20, 695/30, 755LP
Dyes: FITC, PE, ECD, PC5 or PEC5.5, PECy7
Red Laser: 660/20, 725/20, 755 LP
Dyes: APC or Alexa Fluor 647, APC-Alexa Fluor 700, APC-Cy7, APC-Alexa Fluor 750
Violet Laser: 450/50, 550/40
Dyes: Pacific Blue, Krome Orange
Forward Scatter Detector: Fourier design providing up to 3 measurements of forward angle
Side Scatter Detector: High performance photodiode with electronic attenuation
Fluorescence Detectors: FL1 – FL6 Fluorescent Detectors
Flow rates: Continuous pressure is applied to the sample tube based on user selected flow rates: Low, Medium and High
Performance: Carryover: < 0.1%, Dead volume as low as 2 µL when using Beckman Coulter 12 x 75 mm polypropylene tube and adjustable probe
Acquisition modes: 32 tube Multi Carousel Loader (MCL), Single tube sampling mode, Automated work list acquisition, Manual work list mode
Mixing: The MCL vortexes each tube individually before sample acquisition
Barcode reading: Carousel number, tube location, and tube barcode verified to worklist
Biosafety: Biohazard contained wash station thoroughly rinses sample probe
Throughput: 10000 normal leukocytes is 82 tubes/hour
Scatter resolution: Resolves 0.4 micron particles from background noise using Forward Scatter, Maximum detection of up to 40 microns
Fluorescence sensitivity: FITC < 107 MESF, PE < 64 MESF, PC5 < 13 MESF
Acquisition rate: 25000 events per second, 90% yield
Cytometer dimensions (WxHxD): 950 x 610 x 730 mm
Cytometer weight: 104 kg
Supply cart dimensions (WxHxD): 724 x 298 x 495 mm
Supply cart weight: 30 kg
Software included but requires a new licence purchase.
QuestPair is a platform to find and acquire laboratory equipment by connecting research organisations, universities, and companies with high-quality, affordable instruments. The platform facilitates multiple sales daily worldwide and works with partners around the globe to provide trusted, vetted products for a wide variety of scientific needs. Our in-house developed AI algorithms collect data on the usage and availability of scientific instruments, enabling us to provide tailor-made assistance in sourcing suitable laboratory equipment. With a focus on cost-effectiveness and sustainability, QuestPair has an excellent track record in streamlining acquisitions for laboratories to further scientific research and development.
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