Beckman Coulter Navios EX Flow Cytometer

The Beckman Coulter Navios EX Flow Cytometer is equipped with advanced lasers: a Blue Solid State at 488 nm with 55 mW, a Red Solid State Diode at 638 nm with 50 mW, and a Violet Solid State at 405 nm with 80 mW. All lasers are software controlled. It features nominal 90 µm spatially separated beam spots and a 430 µm x 180 µm rectangular channel flow cell. The collection optics are designed with fixed integrated optics and a quartz flow cell, possessing a numerical aperture greater than 1.2.

The detector filters include Forward Scatter at 488/20, with blue laser filters comprising 525/40, 575/30, 614/20, 675/20, 695/30, and 755LP. The supported dyes include FITC, PE, ECD, PC5 or PEC5.5, and PECy7. Red laser filters are 660/20, 725/20, and 755 LP, accommodating dyes like APC, Alexa Fluor 647, APC-Alexa Fluor 700, APC-Cy7, and APC-Alexa Fluor 750. Violet laser filters include 450/50 and 550/40, suitable for dyes such as Pacific Blue and Krome Orange.

The system utilizes a Fourier design forward scatter detector providing up to three forward angle measurements and a high-performance photodiode side scatter detector with electronic attenuation. There are six fluorescence detectors (FL1 – FL6) for enhanced analysis.

Flow rates range from low to high, with continuous pressure applied to the sample tube based on the user’s selection. It ensures a carryover of less than 0.1% and a dead volume as low as 2 µL when using Beckman Coulter 12 x 75 mm polypropylene tubes. Acquisition modes include a 32 tube Multi Carousel Loader (MCL), single tube sampling, automated work list acquisition, and manual work list mode. Each tube is individually vortexed by the MCL before sample acquisition, facilitated by barcode reading of carousel number, tube location, and tube barcode.

Featuring comprehensive biosafety, its biohazard contained wash station thoroughly rinses the sample probe. It offers a throughput of 82 tubes per hour when measuring 10000 normal leukocytes. Remarkable scatter resolution allows resolution of 0.4 micron particles from background noise with a maximum detection of up to 40 microns. Fluorescence sensitivity is measured as FITC < 107 MESF, PE < 64 MESF, and PC5 < 13 MESF. The cytometer can acquire up to 25000 events per second with a 90% yield.

The Navios EX dimensions are 950 x 610 x 730 mm with a weight of 104 kg, accompanied by a supply cart measuring 724 x 298 x 495 mm and weighing 30 kg. Software is included, but a new license purchase is required.