Acea Biosciences NovoCyte 2000 Flow Cytometer

Accuri C6 Flow Cytometer Lab This item was removed from a university where it was surplus to require (more)ment. It is in good cosmetic condition and powers on, we are unable to test it further at our facility. This item has been tested with an in-house 12v power supply. (not included).

Lasers: Blue Solid State: 488 nm, 55 mW, Software Controlled Red Solid State Diode: 638 nm, 50 mW, S (more)oftware Controlled Violet Solid State: 405 nm, 80 mW, Software Controlled Configuration: Nominal 90 µm spatially separated beam spots Flow cell: 430 µm x 180 µm rectangular channel Collection optics: Fixed integrated optics and quartz flow cell design with a numerical aperture of > 1.2 Detector filters: Forward Scatter: 488/20 Blue Laser: 525/40, 575/30, 614/20 , 675/20, 695/30, 755LP Dyes: FITC, PE, ECD, PC5 or PEC5.5, PECy7 Red Laser: 660/20, 725/20, 755 LP Dyes: APC or Alexa Fluor 647, APC-Alexa Fluor 700, APC-Cy7, APC-Alexa Fluor 750 Violet Laser: 450/50, 550/40 Dyes: Pacific Blue, Krome Orange Forward Scatter Detector: Fourier design providing up to 3 measurements of forward angle Side Scatter Detector: High performance photodiode with electronic attenuation Fluorescence Detectors: FL1 – FL6 Fluorescent Detectors Flow rates: Continuous pressure is applied to the sample tube based on user selected flow rates: Low, Medium and High Performance: Carryover: < 0.1%, Dead volume as low as 2 µL when using Beckman Coulter 12 x 75 mm polypropylene tube and adjustable probe Acquisition modes: 32 tube Multi Carousel Loader (MCL), Single tube sampling mode, Automated work list acquisition, Manual work list mode Mixing: The MCL vortexes each tube individually before sample acquisition Barcode reading: Carousel number, tube location, and tube barcode verified to worklist Biosafety: Biohazard contained wash station thoroughly rinses sample probe Throughput: 10000 normal leukocytes is 82 tubes/hour Scatter resolution: Resolves 0.4 micron particles from background noise using Forward Scatter, Maximum detection of up to 40 microns Fluorescence sensitivity: FITC < 107 MESF, PE < 64 MESF, PC5 < 13 MESF Acquisition rate: 25000 events per second, 90% yield Cytometer dimensions (WxHxD): 950 x 610 x 730 mm Cytometer weight: 104 kg Supply cart dimensions (WxHxD): 724 x 298 x 495 mm Supply cart weight: 30 kg Software included but requires a new licence purchase.

The Countess 3 Automated Cell Counter is a benchtop cell counting system equipped with an advanced m (more)achine-learning algorithm, ruggedized optics, fully-automated focus and lighting, and image analysis software for rapid assessment of primary and immortalized cell samples. Capacity: 2 samples per slide Detection Method: Brightfield Memory: On Board, USB Drive Sample Type: Immortalized cell lines & primary cells For Use With (Application): Cell culture & analysis workflows For Use With (Equipment): EVOS microscopes, Attune flow cytometers Dimensions (LxWxH): 229 x 229 x 140 mm Weight: 3.6 kg

Imaging performance Cell size: 5 – 300 microns (Cellometer CHT4-PD300 Slides are required for cells  (more)> 80 microns in diameter) Concentration range: 105 – 107 cells/ml Bright field imaging, fluorescent imaging and pattern-recognition software to quickly and accurately decluster, identify and count individual cells Fluorescent optics Excitation/Emission: 470nm/535nm Example fluorophores: Acridine Orange (+DNA) Calcein AM CFDA SYTO 9 SYTO 13 Excitation/Emission: 540nm/605nm Example fluorophores: Propidium iodide Ethidium bromide Dimensions (WxDxH): 280 x 300 x 330 mm Weight: 11.8 kg

The offered instrument / system is fully functional tested and ready to use. FURTHER DETAILS TO FOLL (more)OW