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Agilent 1260 Infinity II Liquid Chromatography System with Diode Array and Refractive Index Detectors
Product Description
The Agilent 1260 Infinity II LC System w/ DAD & RID is a high-performance liquid chromatography solution designed for advanced analytical workflows in biotechnology, molecular diagnostics, and bioprocessing. This system delivers ultra-fast, high-resolution separations with operating pressures up to 600 bar, compatible with both HPLC and UHPLC columns (2.1, 3, and 4.6 mm ID), supporting flow rates from 0.001 to 10 mL/min.
Key components include:
- Quaternary Pump with gradient mixing using a proprietary high-speed proportioning valve, suitable for a broad flow range including semi-preparative applications.
- Diode Array Detector (DAD) featuring a wavelength range of 190 to 950 nm, utilizing long-life deuterium and tungsten lamps, enabling simultaneous multi-wavelength detection with a fast 120 Hz acquisition rate and precise slit control (1–16 nm). Ideal for multi-analyte UV-visible detection in biotechnology and synthetic biology research.
- Refractive Index Detector (RID) optimized for non-UV absorbing compounds such as carbohydrates, lipids, and polymers, with high sensitivity and a stability-enhancing temperature control from ambient +5 °C to 55 °C, essential for bio-pharmaceutical quality control and molecular cloning workflows.
- Multisampler supporting both vials and microtiter plates with multiwash capability to reduce carryover below 9 ppm, enhancing sample throughput and reliability in clinical diagnostics and biomanufacturing.
- Multicolumn Thermostat (MCT) offering precise temperature control from ambient -10 °C to 85 °C for column thermostatting, supporting enhanced selectivity and speed in chromatographic separations. Bio-inert options available for sensitive bioprocessing applications.
- Integrated degassing unit with four channels ensures solvent purity and system reliability during extended runs.
The system is compatible with Agilent OpenLab CDS Software for comprehensive instrument control, data acquisition, and analysis, supporting automation in research labs focused on molecular diagnostics, synthetic biology, and bioprocess monitoring.
With built-in diagnostics for DAD lamp intensity, pump pressure testing, thermostat performance, and multisampler functionality, this system ensures rigorous performance verification, making it a reliable instrument for complex chromatographic workflows including gel permeation chromatography (GPC) and size exclusion chromatography (SEC).
The Agilent 1260 Infinity II LC System is widely adopted in biotechnology and biopharmaceutical production pipelines, essential for molecular cloning, gene editing analysis, and synthetic biology research requiring precise, sensitive, and reproducible liquid chromatography results.
Item Stats
Product details
| DAD Detector | Specification |
| Detection Type | 1024-element photodiode array |
| Light Source | Deuterium and tungsten lamps |
| Number of Signals | 8 |
| Data Rate | 120 Hz |
| Wavelength Range | 190 - 950 nm |
| Short Term Noise (ASTM) Single and Multi-Wavelength | < ± 0.7 x 10 -5 AU, at 254 and 750 nm |
| Drift | < 0.9 x 10 -3 AU/h at 254 nm |
| Linear Absorbance Range | > 2 AU (5%) at 265 nm |
| Wavelength Accuracy | ± 1 nm |
| Wavelength Bunching | 1 - 400 nm, programmable in 1 nm steps |
| Slit Width | 1, 2, 4, 8, 16 nm |
| Diode Width | ~ 1 nm |
| Flow Cell | Standard: 13 ul volume, 10 mm cell path length and 120 bar (1740 psi) pressure maximum |
| Time Programmable | Wavelength, polarity, peak width, lamp bandwidth, autobalance, wavelength range, threshold, spectra storage mode |
| RID Detector | Specification |
| Detection Type | Refractive Index |
| Refractive Index Range | 1.00 - 1.75 RIU, calibrated |
| Measurement Range | ± 600 x 10^-6 RIU |
| Optics Temperature Control | 5°C above ambient to 55°C |
| Sample Cell | Volume: 8µl Maximum pressure: 5 bar (0.5 MPa) Maximum flow rate: 5 ml/min |
| Valves | Automatic purge and automatic solvent cycle |
| Volumes | Inlet port to sample cell 62 µl Inlet port to outlet port 590 µl |
| Liquid Contact Materials | 316 stainless steel, PTFE and quartz glass |
| pH Range | 2.3 - 9.5 |
| Short Term Noise | < ± 1.25 x 10^-9 RIU |
| Drift | < 200 x 10^-9 RIU/hr |
| Time Programmable | Polarity and peak width |
| Maximum Data Rate | 74 Hz |
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