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Bio-Rad CFX96 Touch Real-Time PCR System with CFX Maestro Software
Product Description
Block Configuration
- 96-well format
Thermal Performance
- Maximum ramp rate: 5.5°C/second
- Temperature uniformity: ±0.4°C well-to-well
- Temperature accuracy: ±0.2°C at critical stages (90°C)
- Six independent thermal electric modules for precise temperature control
- Gradient temperature control ranging 30°C to 100°C
Optical and Detection Features
- Six-channel detection system: five fluorescent colors plus one FRET channel
- Solid-state optical technology eliminates cross talk
- Tailored optical filter sets optimize fluorescence detection for various dyes (e.g., SYBR® Green I, EvaGreen®, FRET)
- Up to five-target multiplexing per well
- Quick scan time: 12 seconds for all channels
Sample Capacity
- Supports sample volumes from 1 µL to 50 µL, optimized for 10–25 µL
User Interface and Software
- Integrated LCD touchscreen for stand-alone operation without a computer
- CFX Maestro Software for experiment design, data collection, and analysis via connected PC
- Software features include statistical analysis (t-tests, ANOVA), graphical representation (bar charts, clustergrams, volcano plots), multi-plate study analysis, and primer/assay integration
- USB and direct email data export available
- Dye calibration recommended per application
This system is engineered for precise, high-throughput real-time PCR applications, enabling sensitive nucleic acid quantification with robust multiplexing capabilities and flexible experimental protocols.
Item Stats
Product details
| Block Configuration | 96-Well (1 to 50 uL reactions, recommended for 10 to 25 uL reactions) |
| Temperature Range | 0°C to 100°C |
| Temperature Uniformity | ± 0.4°C well-to-well within 10 seconds of arrival at 90°C |
| Temperature Accuracy | ± 0.2°C of preprogrammed target at 90°C |
| Maximum Ramp Rate | 5°C/second |
| Average Ramp Rate | 3.3°C/second |
| Run Time | > 30 seconds |
| Gradient Operational Range | 30°C to 100°C |
| Gradient Programmable Span | 1°C to 24°C |
| Scan Time | 12 seconds (all channels), 3 seconds (FAM/SYBR Green only) |
| Excitation Source | 6 filtered LEDs |
| Detection Channels | 6 filtered photodiodes |
| Range of Excitation/Emission Wavelengths | 450 to 730 nm |
| Dynamic Range | 10 orders of magnitude |
| Sensitivity | Detects 1 copy of target sequence in human genomic DNA |
| Multiplexing | Up to 5 targets per well |
| Dimensions (W x D x H) | 13 x 18 x 14 in. (33 x 46 x 36 cm) |
| Weight | 47 lb. (21 kg) |
| Electrical Requirements | 100-240V, 50/60Hz, 850W |
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