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Becton Dickinson FACS Canto II Flow Cytometer with Multi-Laser and 8-Color Fluorescence Detection
Product Description
The Becton Dickinson FACS Canto II is a sophisticated laser-based flow cytometer designed for advanced cell analysis and fluorescence detection applications in biotechnology and clinical diagnostics. Equipped with a standard 488 nm solid-state laser (20 mW) and a 633 nm HeNe laser (17 mW), this instrument supports up to 8-color fluorescence detection using a 2–3 laser configuration, enabling comprehensive multi-parametric cell analysis.
Key technical features include:
- Orthogonal flow cell made of quartz (180 × 430 µm), optimized for precise sample interrogation.
- High sensitivity detection with scatter thresholds as low as 0.5 µm (SSC) and fluorescence sensitivities below 100 MESF for FITC and 50 MESF for PE, suitable for delicate molecular diagnostics and gene editing research where detection sensitivity is critical.
- Sample throughput of approximately 10,000 events per second covering 6 fluorescence channels plus 2 scatter detectors, making it ideal for high-throughput bioprocessing workflows.
- Low carryover (≤0.1%) and efficient sheath fluid consumption (1.10 L/h during measurement) to ensure reliable data integrity and economical operation.
- Robust digital signal processing with an 18-bit dynamic range and advanced compensation matrix for fluorescence channel correction supports complex multi-label experiments widely adopted in synthetic biology and molecular biology research.
- Environmental operational specifications allow installation in typical laboratory climates with 20–80% humidity and temperatures from 16 to 31 °C during operation.
Physically, the instrument’s footprint measures 91 cm (W) × 61 cm (D) × 64 cm (H) without a loader, weighing 146 kg for the main unit plus an additional 51 kg for the flow path cart. Power requirements are AC 100/115/230 V with consumption around 500 W.
This used equipment is sold as-is without a loader, and its performance may vary from original factory specifications. The FACS Canto II is a valuable tool for biomedical researchers requiring precise and versatile flow cytometry for biomanufacturing, molecular diagnostics, cell imaging, and clinical research applications.
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