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Hamamatsu ImagEM X2 C9100-23B High-Speed EM-CCD Camera
Product Description
The Hamamatsu ImagEM X2 C9100-23B is a high-performance EM-CCD camera offering the following features and specifications:
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Sensor & Imaging
- Electron Multiplying Back-Thinned Frame Transfer CCD
- Effective pixels: 512 x 512
- Pixel size: 16 µm x 16 µm
- Effective area: 8.19 mm x 8.19 mm
- EM gain range: 4 to 1200 (typical)
- Photon Imaging mode for ultra-low light with improved resolution and linearity
-
Speed & Frame Rates
- Pixel clock rates: 22 MHz, 11 MHz, 0.6875 MHz
- Full frame readout speed: 70.4 frames/s at 22 MHz
- Up to 1076 frames/s using analog binning and regions of interest (ROI)
- Enhanced corner readout technology for faster ROI imaging
-
Noise & Sensitivity
- Extremely low dark current (0.0005 e⁻/pixel/s at -80°C water cooling)
- Readout noise (typical RMS):
- 36 electrons at EM gain 4, 22 MHz
- 1 electron max at EM gain 1200, 22 MHz
- High quantum efficiency (QE) and minimal dark noise with stable cooling
- Clock induced charge (CIC) minimized by optimized sensor drive
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Cooling System
- Hermetic vacuum-sealed air/water-cooled camera head
- Cooling temperature stability ±0.01°C
- Forced-air cooling: down to -65°C stabilized (max -80°C typical)
- Water cooling: down to -80°C stabilized (max -100°C typical)
- Cooling reduces dark current and noise
-
Mechanical & Interface Features
- Integrated software-controlled mechanical shutter for EM gain protection
- Connectivity via IEEE 1394b interface
- Four SMA ports for external trigger input and output, including programmable triggers and delays
- C-mount lens interface
- 16-bit analog-to-digital conversion
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Real-time On-board Image Processing
- Background subtraction
- Shading correction
- Recursive filter (noise reduction)
- Frame averaging
- Spot noise reducer (cosmic ray noise elimination)
(Note: Recursive filter, frame averaging, and spot noise reducer cannot be used simultaneously)
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Operational & Environmental
- Exposure time:
- Internal sync mode from 13.9 ms to 2 h depending on clock
- External trigger mode from 10 µs to 2 h
- Operating temperature: 0°C to +40°C (performance guaranteed 0°C to 30°C)
- Storage temperature: -10°C to +50°C
- Max 70% humidity, no condensation
- Power: AC 100-240 V, 50/60 Hz, approx. 140 VA
- Exposure time:
-
Additional Features
- EM gain protection with warning and protection modes to prolong sensor life
- EM gain recalibration via software (DCAM-API supported)
- Direct electron output signal display in electrons (not AD counts)
- Black/White clip function to set intensity thresholds
- Stable digitizer offset providing baseline stability over time
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Applications
- Protein-protein interaction studies
- Calcium wave monitoring in cells
- Real-time spinning disk confocal microscopy
- Single molecule TIRF microscopy
- In vivo fluorescence blood cell imaging
- Luminescence gene expression imaging
This camera is designed for ultra-low light level imaging with high speed and high precision, suitable for demanding biological and physical research requiring quantitative fluorescence and photon counting under minimal light conditions.
Item Stats
Product details
| Binning | Effective vertical width (Sub-array) |
| 512 | 256 |
| 1_1 | 70.4 |
| 2_2 | 131 |
| 4_4 | 231 |
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