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Thermo Fisher Scientific EVOS M5000 Benchtop Fluorescence Imaging System
Product Description
The Thermo Fisher Scientific EVOS M5000 Imaging System is an advanced benchtop fluorescence microscope optimized for precise cell imaging in biotechnology, molecular diagnostics, and biomedical research applications. It supports multiple imaging modalities including fluorescence (with GFP, RFP, DAPI filter cubes), phase contrast, brightfield, and color brightfield imaging, allowing for comprehensive visualization in live-cell imaging, synthetic biology, and bioprocessing workflows.
Key features include:
- Four-color fluorescence capability with up to four LED cubes, providing stable, mercury-free illumination with a >50,000-hour lifetime.
- 3.2 MP monochrome CMOS camera with 3.45 µm pixel size capturing high-resolution, 16-bit RAW images essential for quantitative fluorescence analysis.
- Automated Z-stack acquisition and time-lapse imaging support critical for dynamic cellular studies and biomanufacturing monitoring.
- Infinity-corrected optical system and a five-position manual objective turret allowing integration of over 30 high-quality long working distance/cover slip corrected objectives (1.25x to 100x magnification) to accommodate diverse experimental needs.
- Automated focus mechanism providing sub-micron precision (0.15 µm), enhancing image consistency in longitudinal live-cell imaging and synthetic biology assays.
- Onboard software tools for autofocus, cell counting, confluence and transfection efficiency measurements, image annotation, and batch processing streamline molecular biology workflows and data analysis.
- Integrated 18.5-inch articulated LCD monitor with built-in control interface facilitates workflow efficiency in core imaging facilities and clinical diagnostic laboratories.
- Flexible image storage and connectivity options include internal SSD, USB 3.0 drives, and Ethernet with network/cloud integration via Thermo Fisher Connect platform, enabling remote data access critical in modern biopharmaceutical production pipelines.
- Mechanical stage with sub-micron control travel range (120 x 80 mm), suitable for precise sample positioning in live-cell experiments and tissue imaging.
- Plug-and-play design eliminates lamp warm-up, calibration, and alignment downtime, ideal for high-throughput environments and biomanufacturing quality control.
This compact, all-in-one imaging platform is widely adopted in molecular cloning workflows, cell culture labs, and synthetic biology research, making it essential for researchers focused on fluorescence analysis, cell imaging, and biomedical engineering. The EVOS M5000 offers a robust solution for fluorescence microscopy paired with user-friendly automation critical in molecular diagnostics and biopharmaceutical production pipelines.
Item Stats
Product details
| Imaging modes | Fluorescence, brightfield, color brightfield, and phase contrast. |
| Imaging methods | Single color, multicolor, time-lapse, Z-stacking, and movie capture. |
| Optical system | Infinity-corrected optical system; RMS-threaded objectives with 45 mm parfocal distance. |
| Illumination | Adjustable-intensity LED cubes (>50,000-hour lifetime) with integrated hard-coated filters. |
| Light cube capacity | 4-position chamber for up to 4 EVOS fluorescent LED cubes. |
| Objective capacity | 5-position manual turret (not automated). |
| Objectives | Selection from more than 30 high-quality, long working distance (LWD) and coverslip-corrected (CC) objectives; magnification from 1.25x to 100x. (sold separately see includes section for details) |
| Condenser | 60 mm LWD condenser; 4-position turret with a clear aperture and 3-phase annuli. |
| Focus mechanism | Automated focus with sub-micron (0.15 µm) resolution and single-step accuracy. |
| Camera High-sensitivity | 3.2 MP monochrome CMOS camera (2,048 x 1,536 pixels) with 3.45 μm pixel resolution. |
| Captured images | 16-bit RAW monochrome: TIFF, PNG (12-bit dynamic range) 8-bit color: TIFF, PNG, JPG Movies and time-lapse: AVI, WMV |
| LCD display | 18.5-inch high-resolution articulated LCD monitor. |
| Stage control | Mechanical |
| x-axis and y-axis control | Fine positioning control; travel range: 120 x 80 mm with sub-micrometer resolution. |
| z-axis control | Automated, motorized z-axis software control. |
| Integrated onboard operating software | Autofocus Cell counting Confluence measurements Transfection efficiency measurements Z-stacking with multichannel composite overlay Batch analysis Annotation tool |
| Image saving | Save images on the embedded hard drive, an external USB device, or a local network. |
| Computer Embedded PC with 4 GB RAM. | Computer Embedded PC with 4 GB RAM. |
| Storage 10 GB SSD or 16 GB USB 3.0 memory stick. | Storage 10 GB SSD or 16 GB USB 3.0 memory stick. |
| Output ports | Power; 4 USB 2.0 ports; 1 USB 3.0 port; 1 display port; 1 RJ45 port. |
| Networking capability | Connect via the Microsoft SMB protocol using an Ethernet cable or the USB 3.0 WiFi dongle (included). |
| Cloud connectivity | Connect to the Thermo Fisher Connect Platform for remote access to images and data via the internet. |
| Dimensions | 20x20x23 |
| Power | 120V |
| Weight | 56lbs |
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