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Nikon Ti2-E PFS N-SIM Super-Resolution Inverted Motorized Microscope with Dual Fluorescence Illumination
Product Description
The Nikon Ti2-E PFS N-SIM microscope is a motorized, inverted fluorescence system optimized for super-resolution structured illumination microscopy (SIM) and advanced multi-channel fluorescence imaging. It integrates dual fluorescence illumination paths combining a LU-N3-SIM laser-based unit for structured illumination and a Lumencor Sola LED light source for widefield applications, supporting comprehensive fluorescence workflows in molecular diagnostics and bioprocessing research.
Key features include:
- Microscope Base & Control: Motorized Ti2-E inverted frame with TI2-CTRE controller enables automation of stage, focus, objective turret, and filter switching facilitating repeatable high-throughput imaging.
- Imaging & Optics: Equipped with Nikon Plan Apo λ 10x/0.45, S Fluor 20x/0.75, and Apo 60x/1.40 Oil λS objectives for high numerical aperture imaging essential in biotechnology and live-cell imaging. The 6-position motorized objective turret and 6-position motorized condenser expand optical flexibility.
- Fluorescence System: Two motorized 6-position filter cube turrets and a motorized emission filter wheel carrying emission filters EM525/50, EM605/70, and EM700/75 enable rapid multi-wavelength channel switching critical for gene editing assays and synthetic biology imaging. Optional new filter sets for DAPI, FITC, Texas Red, Cy3, and Cy5 dyes address a wide range of fluorescence labels used in molecular cloning and immunostaining.
- Stage & Focus: Motorized XY stage with MCL Nano-Drive piezo and PI E-753 piezo controller supports fast Z-stack acquisition and multi-position timelapse essential for biomanufacturing and real-time cellular analysis. The integrated Perfect Focus System (PFS) maintains stable focus over long experiments, reducing drift in time-lapse and live-cell applications.
- Camera & Software: Hamamatsu ORCA-Flash4.0 monochrome sCMOS camera delivers high sensitivity and resolution. The desktop computer with NIS-Elements AR software allows multidimensional acquisition (XYZ, time, fluorescence channels), automated tile scan stitching, and hardware synchronization, enabling complex experimental designs needed in molecular diagnostics and fluorescence analysis.
- Additional Features: Environmental enclosure for airflow and thermal stability supports consistent imaging conditions. Customizable configurations are possible for enhanced fluorescence illumination sources, filter sets, objectives, and advanced CCD or EMCCD cameras.
- Applications: The microscope system is widely used in biotechnology fields including live-cell imaging, fluorescence analysis, immunocytochemistry, synthetic biology research, high-content screening, and biopharmaceutical production pipelines requiring super-resolution and multi-position automated imaging.
This Nikon Ti2-E PFS N-SIM microscope is designed to support critical workflows in cell imaging and molecular diagnostics through automated multi-dimensional fluorescence microscopy with super-resolution capabilities. It is modular and customizable for diverse research applications including gene editing validation, bioprocessing quality control, and synthetic biology experimentation. The system is verified for optical performance, fluorescence intensity, and mechanical precision, ensuring reliable operation for advanced biomedical and biotechnology research environments.
Item Stats
Product details
| Stock | In Stock |
| Brand | Nikon |
| Product Code | 61818-V2 |
| Condition | Pre-Owned |
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