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Nikon Eclipse Ti-E PFS Motorized Inverted Fluorescence and Phase Contrast Microscope with LED Illumination and Motorized XY Stage
Product Description
The Nikon Eclipse Ti-E PFS is a motorized inverted fluorescence microscope engineered for advanced live-cell imaging, fluorescence analysis, and phase contrast applications widely used in biotechnology, molecular diagnostics, and cellular research. This system integrates a motorized XY stage, motorized Z-axis focus drive, and Nikon’s Perfect Focus System (PFS) for real-time automated focal drift compensation, minimizing phototoxicity during long-term time-lapse and multi-position imaging.
Key technical features include:
- Microscopy techniques: Brightfield with phase contrast, fluorescence imaging with LED illumination, and automated focus control via the PFS optical feedback system.
- Fluorescence illumination: Lumencor LED light source with a collimating adapter and motorized 6-position filter cube turret supporting interchangeable filter sets (DAPI, FITC/EGFP, Texas Red, Cy3, Cy5).
- Objectives: High-quality Plan Fluor and Plan Apo VC objectives (4x/0.13, 10x/0.30, 20x/0.75) for high-resolution imaging in biotechnology workflows such as gene editing and synthetic biology.
- Stage and automation: Motorized XY stage with joystick controller and automated parfocality adjustment allowing precise Z-stack acquisition and multi-well plate scanning critical in molecular biology and bioprocessing.
- Camera and software: Equipped with a Bestscope 8.3MP color CMOS camera; software package includes open-source MicroManager integrated with ImageJ for multidimensional image acquisition, time-lapse, and quantitative analysis vital for neuroimaging and pharmacological assays.
- Optical and mechanical quality checks: Comprehensive preventive maintenance including optics inspection for field distortion and fluorescence intensity verification at the specimen plane ensure consistent performance for biomedical engineering and drug response studies.
The motorized automation components enhance throughput and reproducibility in experimental protocols requiring stable long-term observations, such as live-cell dynamics, neuronal activity tracking, and biopharmaceutical production pipelines. The PFS uses infrared light to continuously monitor the interface between cover glass and sample, allowing real-time focus corrections which reduce photobleaching and phototoxicity.
This microscope supports customization with additional fluorescence filters, advanced camera options (CCD, sCMOS, EMCCD), higher magnification apochromat objectives, and illumination upgrades (Xenon, Metal Halide) adapting to diverse research demands from molecular biology to clinical diagnostics.
In summary, the Nikon Eclipse Ti-E PFS is a versatile, motorized inverted fluorescence microscope system designed for high-precision imaging tasks instrumental in synthetic biology, molecular cloning workflows, and real-time cellular bioprocess monitoring, supporting cutting-edge applications in biotechnology, neuroscience, and biomedical research.
Item Stats
Product details
| Stock | In Stock |
| Brand | Nikon |
| Product Code | SM100196-MG1XYPFSPH |
| Condition | Pre-Owned |
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Northeastern UniversityFrom €21,273.28*ex. VAT
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