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Molecular Devices SpectraMax M3 Multimode Microplate Reader with Tunable Monochromators
Product Description
The Molecular Devices SpectraMax M3 is a versatile multimode microplate reader designed for diverse biochemical and cell-based assay formats in biotechnology, molecular diagnostics, and bioprocessing workflows. It supports absorbance, fluorescence intensity, and luminescence measurements on a single platform, facilitating seamless integration into molecular biology and synthetic biology research pipelines.
- Tunable monochromators with 1 nm wavelength increments provide precise spectral optimization over a broad range:
- Absorbance: 200–1000 nm
- Fluorescence and Luminescence: 250–850 nm
- Equipped with dual photomultiplier tube (PMT) detectors to deliver high sensitivity and dynamic range critical for low-concentration biomolecule detection.
- Compatible with multiple plate formats (6–384 wells) and cuvettes, enhancing flexibility for various throughput needs in biomanufacturing and gene editing assay development.
- Features both top and bottom reading modes, optimizing detection sensitivity for suspension and adherent cell assays, essential for live-cell imaging and cell-based fluorescence analysis.
- Robust temperature control from 2°C above ambient to 60°C with <1°C uniformity ensures stable assay conditions, supporting sensitive enzyme kinetics and cell culture studies.
- Fast read times (e.g., ~18 seconds for absorbance in 96-well plates) and 100+ integrated protocols accelerate experimental workflows in molecular cloning and clinical diagnostics.
- Robotic compatibility allows automation integration, suitable for high-throughput biopharmaceutical production and screening applications.
This reader is engineered for reliability and precision, meeting the stringent needs of biotechnology research and clinical laboratories. The SpectraMax M3’s combination of tunable detection, flexible assay accommodation, and validated performance makes it widely adopted in molecular diagnostics and essential for workflows involving fluorescence-based assays and luminescence measurements.
Item Stats
Product details
| Robotic-compatible | Yes |
| Plate formats | 6, 12, 24, 48, 96, 384 wells |
| Light source | Xenon Flash Lamp (1 joule/flash) |
| Detectors | 2 photomultiplier tubes (PMT) |
| Shaker time | 0 to 999 seconds |
| Temp. control | 2°C above ambient to 60°C |
| Temp. uniformity | < 1°C at 37°C set point |
| Temp. accuracy | ±1°C at 37°C set point |
| Readings | Endpoint, Kinetic Spectral Scanning |
| Standard Read Times (minutes:seconds)* 96 wells 384 wells | Absorbance 0:18 0:49 Fluorescence Intensity 0:17 0:48 Luminescence 2:00 7:00 |
| Wavelength range | 200–1000 nm |
| Wavelength selection | Monochromator, tunable in 1.0 nm increments |
| Wavelength bandwidth | 4.0 nm |
| Wavelength accuracy | ±2.0 nm |
| Wavelength repeatability | ±0.2 nm |
| Photometric range | 0–4.0 OD |
| Photometric resolution | 0.001 OD |
| Photometric accuracy | (microplate) < ±0.006 OD ±1.0%, 0–2 OD (cuvette) < ±0.005 OD ±1.0%, 0–2 OD |
| Photometric precision | < ±0.003 OD ±1.0%, 0–2 OD |
| Stray light | < 0.05% @ 230 nm |
| Fluorescence Intensity Performance | Top or bottom |
| Wavelength range | 250–850 nm |
| Wavelength selection | Monochromators, tunable in 1.0 nm increments |
| Bandwidth (EX, EM) | 9 nm, 15 nm |
| Optimized sensitivity | ? 1 pM fluorescein in 96 wells, ? 1.5pM in 384 wells |
| Guaranteed sensitivity | < 5 pM fluorescein in 96 wells or cuvette < 20 pM in 384 wells |
| Luminescence Performance | Top or Bottom |
| Wavelength selection | Choice of simultaneous detection of all wavelengths or selection via monochromator, tunable in 1.0 nm increments |
| Wavelength range | 250–850 nm |
| Optimized sensitivity | 43pM ATP in 96 wells |
| Guaranteed sensitivity | 75pM ATP in 96 wells |
| Dynamic range | > 6 decades |
| Cross-talk | < 0.3% in white 96- and 384-well Microplates |
| Dimensions | 23 x 16 x 9 inches |
| Weight | 36 lbs |
| Power | < 420 watts, 100–240 VAC, 3.5 A, 50/60Hz |
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