BTX HT-200 Electroporation Plate Handler

The Nucleofector technology is a major improvement of the well known electroporation technology. The (more) Nucleofector technology involves a combination of optimized electrical parameters and specific Nucleofector solutions for different cell types. This combination permits the crucials step: delivery of the DNA of interest straight into the nucleus. As the DNA quickly reaches the nucleus, expression can begin without delay. The nucleofected cells are thus ready for analysis after a short incubation time of only 2 – 8 hours. DNA quality: 1 – 5 µg plasmid concentration per nuclefection sample (100 µl), plasmid concentration can be increased up to 10 µg per sample. This may lead to higher gene transfer efficiencies, but at the same time may increase mortality of the cells. Cell density: 2 x 105 – 1 x 107 cells per nuclefection sample for suspensions cells 2 x 105 – 2 x 106 cells per sample for adherent cells Cell densities of < 2 x 105 cells per smaple are not advisable beacause the gene transfer efficiency may decrease and mortality may increase. Dimensions (WxDxH): 300 x 230 x x110 mm Weight: 2.5 kg

The Gemini X2 Systems is designed for researchers who need the ultimate experiment flexibility. In o (more)ne easy setup, square wave and exponential decay waves can be applied to cells in any format. These systems incorporate remote operation functionality via footswitch or PC and internal log storage of experiment data for easy optimization, quality control requirements and troubleshooting. Electroporation of suspension cells can be achieved in 96-well plates with the HT plate handler. Usable with all cell electroporation Features: Square Waveform, Multi-Pulsing Square Wave, Exponential Decay Waveform, Multi-Pulsing Exponential Decay, Resistance/Pulse Monitoring, Experiment Log Storage, Preprogrammed Protocols, Unlimited Custom Protocol Storage, Remote Operation, PC Communications Voltage Range: 5 to 3000 V Pulse Width Range: 10 μs to 5 s Pulse Interval: 100 ms to 30 s Dimensions (DxWxH): 324 x 286 x 216 mm Weight: 7.3 kg

This BTX Electro Square Porator ECM 830 was removed from a university lab where it was surplus to re (more)quirements. The item powers on and is in reasonably good cosmetic condition, we are unable to test it further at our facility.

Electroporator Lonza 4D-Nucleofactor System. Formed by two modules: unit Core (control unit) and un (more)it-X unit-transfection). Unit X performs 16-to 32-transfections per minute. Includes instruction manual and original packaging. 2014. Specifications: 240 V; 50-60 Hz The Core Unit - Controlling the 4D-Nucleofactor™ System The X unit – Supporting Nucleofection™ of various cell numbers in different formats

Mini heating plates and magnetic stirrers This ruggedly built OHAUS series of small instruments can  (more)heat and mix up to 1,000ml of water on their 102 x 102mm ceramic plate. They are perfect for school labs and other applications that require simple shaking and heating on a daily basis. All models are equipped with a support rod hook with a locking knob that accommodates the optional support rod kit. Stir models include a 38mm PTFE coated magnetic stir bar. Available models: Model with heating: up to 500°C. Fixed temperature model: 500°C. Model with stirring: 500°C. Model with heating and stirring: stirring from 100 to 1200 rpm; temp. up to 500°C. Applications Boiling water, solubilization buffers and reagents, preparation media, concentration samples Operation Detachable three-wire cable and plug (included) Structure Valox armor, knobs with basic controls, 3.8cm stir bar (included with stir models), On / Off toggle switch (fixed temperature models) ___________ Not sure which model to choose? Download the Ohaus Hotplate and Shaker Selection Guide for a quick overview of all available models.

Temperature range: 3°C to 16°C Maximum fluctuation: 3.6 °C Maximum gradient: 4.1 °C Defrosting syste (more)m: Automatic defrosting system Cooling system: Dynamic Capacity: 344 L Dimensions (HxWxD): 1840 x 597 x 615 mm Internal dimensions (HxWxD): 1635 x 440 x 435 mm Weight: 65 kg

LabCaire TC 1200 Class II Safety Cabinet Lab This was removed from a laboratory where it was surplus (more) to requirements. The unit powers up and is in full working order. It is in good cosmetic condition, with some minor marks from use. (Socket tester not included) I

This Nikon Optiphot-2 Microscope was removed from a university lab where it was surplus to requireme (more)nts. The unit powers up and all the objectives appear to focus well. It is in good cosmetic condition with only minor marks from use. There are 4 x Objectives included, and they are as follows; Objectives: Nikon 320081 Japan M Plan 100 1.25 oil 210/0Nikon 140131 Japan M Fluor 40/1.0 Oil 210/0Nikon 350730 Japan M Plan 10 0.25 210/0Nikon 120058 Japan M Fluor 20/0.6oil 210/0 Nikon PSM-2120

The newest line of Radwag Mass Comparators enables adjusting mass standards and weights according to (more) the OIML recommendations (R-111) from 1 mg to 200 g for F2 class and lower. The comparators are used both for ensuring traceability of mass measurements, and verification of weights in accordance with the principles of legal metrology. RADWAG Mass Comparators have gained recognition among Accredited Calibration Laboratories in many countries. Mass comparator XA 5Y.KO series comprises two components. One of them holds the electronic module, and the other precise mechanical measuring system. The comparator features an aesthetic, large weighing chamber and a glass draft shield in one. Its asset is an automatic door opening system. The XA 5Y.KO series features an intuitive menu supporting a user in instrument operation.

Labconco FreeZone Bulk Tray Dryer with 6-Port Manifold 115V,50/60Hz FreeZone Bulk Tray Dryers lyophi (more)lize moderate to large bulksample loads or numerous small batches of vials or serum bottles. The Bulk TrayDryers have three large, 12.7” x 16.6” shelves that heat up to +60C. Optionsinclude a 6-port manifold, isolation valve, additional heated shelves andsample trays. Lyo-Works™ OS lets the user set up to 30 programs, with up to 16steps each; record, chart and store data; and receive email alerts. The traydryer connects to a FreeZone Console Freeze Dryer that provides a real timedisplay of the tray dryer’s parameters on a large capacitive touch screen. Features Provides precise control and program flexibility for consistent performance Three 12.7 x 16.6 in. heated shelves with sensors for monitoring sample temperature provide 632 sq. inches of shelf space Microprocessor maintains system temperature within 3C of set point Clear acrylic viewing door provides complete visibility of shelves Lyo-Works™ OS provides flexible programming and control with real time display of freeze dryer and tray dryer parameters on 5 in. full color touch screen on connected FreeZone 6, 12, or 18L Freeze Dryer (sold separately) One-touch data logging and display provides information to maximize sample quality and minimize run time INCLUDED: The Labconco 780601100 FreeZone Bulk Tray Dryer with 6-Port Manifold 115V,50/60Hz includes: - Communication Cable - Hardware (Screws x 8, Nuts x 4, Washers x 8) - Power Cord - Operator's Guide - Manufacturer's Documentation - 1 Year Manufacturer's Warranty