BTX HT-200 Electroporation Plate Handler

The Nucleofector technology is a major improvement of the well known electroporation technology. The (more) Nucleofector technology involves a combination of optimized electrical parameters and specific Nucleofector solutions for different cell types. This combination permits the crucials step: delivery of the DNA of interest straight into the nucleus. As the DNA quickly reaches the nucleus, expression can begin without delay. The nucleofected cells are thus ready for analysis after a short incubation time of only 2 – 8 hours. DNA quality: 1 – 5 µg plasmid concentration per nuclefection sample (100 µl), plasmid concentration can be increased up to 10 µg per sample. This may lead to higher gene transfer efficiencies, but at the same time may increase mortality of the cells. Cell density: 2 x 105 – 1 x 107 cells per nuclefection sample for suspensions cells 2 x 105 – 2 x 106 cells per sample for adherent cells Cell densities of < 2 x 105 cells per smaple are not advisable beacause the gene transfer efficiency may decrease and mortality may increase. Dimensions (WxDxH): 300 x 230 x x110 mm Weight: 2.5 kg

Electroporator Lonza 4D-Nucleofactor System. Formed by two modules: unit Core (control unit) and un (more)it-X unit-transfection). Unit X performs 16-to 32-transfections per minute. Includes instruction manual and original packaging. 2014. Specifications: 240 V; 50-60 Hz The Core Unit - Controlling the 4D-Nucleofactor™ System The X unit – Supporting Nucleofection™ of various cell numbers in different formats

The Gemini X2 Systems is designed for researchers who need the ultimate experiment flexibility. In o (more)ne easy setup, square wave and exponential decay waves can be applied to cells in any format. These systems incorporate remote operation functionality via footswitch or PC and internal log storage of experiment data for easy optimization, quality control requirements and troubleshooting. Electroporation of suspension cells can be achieved in 96-well plates with the HT plate handler. Usable with all cell electroporation Features: Square Waveform, Multi-Pulsing Square Wave, Exponential Decay Waveform, Multi-Pulsing Exponential Decay, Resistance/Pulse Monitoring, Experiment Log Storage, Preprogrammed Protocols, Unlimited Custom Protocol Storage, Remote Operation, PC Communications Voltage Range: 5 to 3000 V Pulse Width Range: 10 μs to 5 s Pulse Interval: 100 ms to 30 s Dimensions (DxWxH): 324 x 286 x 216 mm Weight: 7.3 kg

The Gene Pulser Xcell System is a flexible, modular electroporation system for transfecting every ce (more)ll type from primary, suspension, and difficult-to-transfect cells, including T cells, to bacteria and fungi. The system is composed of a main unit, two accessory modules, the capacitance extender (CE module) and the pulse controller (PC module), and a ShockPod cuvette chamber. Features and Benefits Universal electroporation — transfects all cell types, from primary, stem, and T cells to bacteria and fungi Flexibility with your experiment parameters for the highest transfection efficiency and viability Preset protocols — ready-to-use preoptimized protocols for frequently used mammalian and bacterial cell types Manual operation — allows for entry and storage of new parameters or editing of all parameters Optimization protocols — enables the best conditions to be determined using incremental voltage steps Exponential and square waveforms — allows for optimal delivery Confidence in your results — uses PulseTrac circuitry and arc-protection features to ensure sample protection and reproducible results Protocol library — collection of electroporation protocols for every cell type including primary, immortal, and bacterial cells Prevalidated published protocols — saves time optimizing conditions Easily accessed data management — storage and recall of parameters and results from previous 100 experiments, useful for troubleshooting Output Waveform: Exponential or square Output Voltage: 10 – 3,000 V Capacitance: 10 – 500 V, 25 – 3,275 µF in 25 µF increments, 500 – 3,000 V, 10, 25, 50 µF Resistance (parallel): 50 – 1,000 Ω in 50 Ω increments, plus infinity Sample resistance: 20 Ω minimum at 10 – 2,500 V, 600 Ω minimum at 2,500 – 3,000 V Square-wave timing: 10 – 500 V: 0.05 – 10 ms in 0.05 ms increments, 10 – 100 ms pulse in 1 ms increments, 1 – 10 pulses, 0.1 – 10 sec interval/ 500 – 3,000 V: 0.05 – 5 ms in 0.05 ms increments, 1 – 2 pulses, 5 sec minimum interval Dimensions (WxDxH): 310 x 300 x 280 mm Weight: 11.6 kg

Lauda Eco Gold with Lauda Eco RE 420 Water Bath This was removed from a university lab where it was  (more)surplus to requirements. The unit powers up and is in full working order (Thermometer used for testing, not included). It is in good cosmetic condition with minor marks.

Samples and applications - Food homogenization and preparation of spreadable creams and stuffing for (more) foods (e.g. meat, vegetables) - Preparation of samples for DNA analysis, organic tissue samples and dispersions of organic tissues - Homogenisation of tobacco leaves - Processing of textile fibres - Sterilization and cleaning methods: it is possible to sterilize all the components of the dispersing tool in different ways according to the construction materials and the sample processed TECHNICAL DATA - Rotor diameter: 15 mm - Stator diameter: 20 mm - Stator/rotor gap: 0.5 mm - Volume (H2O): 50 - 2,000 ml - Immersion depth: 40-175 mm - Max. speed: 25,000 rpm - Max. circumferential speed: 19.6 m/s - Ultimate fineness, emulsions: 1-7 µm - Ultimate fineness, suspensions: 5-40 µm - Max. allowable working temperature: 180 °C - Materials in contact with the medium: AISI 316L, PTFE - Suitable for solvents: Yes

Thermal shaker with Ohaus incubator OHAUS thermal agitators are designed for applications that requi (more)re constant and precise high-speed shaking, with heating or cooling. The temperature control allows to reach 17°C below the ambient temperature (for the model with cooling) up to 100°C. In case of high accuracy, the temperature can be calibrated by the user. Thanks to interchangeable blocks it is possible to customize the instrument for different applications, housing test tubes or microplates. The intuitive touchscreen allows the operator to view all program steps and monitor the entire process with a simple glance. - Best-in-class thermal performance thanks to advanced electronics and software. Up to 17°C below ambient temperature. - The intuitive touchscreen simplifies use and programming, with complete functions and help screens in six different languages, to assist the operator while using the unit - Easy creation of programs for repetitive applications. Memory for five different five-step programs. The USB port allows you to transfer test data to a flash drive for data collection and program storage Applications DNA, RNA, cell cultures, hybridizations, protein studies Display 4.3-inch (10.9 cm) color liquid crystal touchscreen (LCD) display, programmable in six languages Operation Detachable three-wire cable and plug (included). The unit requires a lock. Compatible with Eppendorf Thermomixer® R blocks Communication The USB port guarantees great flexibility in data collection and transfer Structure Cold-touch Valox construction, removable plastic rack, cover and screwdriver for block removal (included) Construction features Hot Cover Indicator, Audible Alarm, Maximum Temperature Limitation Function, Temperature Priority Function, Pulse / Fast Mix Mode, Temperature Calibration Mode, Temperature Rise Rate Function Technical Specifications - ISTHBLCTS Calibration: temperature calibration Transmission System: Brushless DC Motor Heating rate: 5°C/min Internal dimensions (HxLxW): 14.5cm x 28.7cm x 21.1cm Speed ​​Accuracy: ±2% Temperature accuracy (±): +/- 0.5°C between 20°C and 45°C; +/-2°C between 20°C and 45°C

The offered instrument / system is fully functional tested and ready to use. FURTHER DETAILS TO FOLL (more)OW

incl. 3 holders:

High Capacity HRP Platform Wherever high readability and precision are important. Wherever good repe (more)atability in harsh industrial conditions matters. High Capacity HRP Platforms are the ideal solution for any type of industry. Repeatability that cannot be repeated by others High Capacity HRP Platforms have a built-in internal adjustment system. Automatic internal adjustment guarantees the best quality of work and repeatability of results. You save time and avoid the inconvenience of manual adjustment. Unbreakable High Capacity HRP Platforms feature a modern electromagnetic mechanism design in a sealed steel housing and overload protection. The use of stainless steel for the pan guarantees you a long life of the platform. Fearless of its contact with moist objects or accidental splashing. High Capacity HRP Platforms are distinguished by a high degree of protection IP 67. For an additional charge we will make HRP platforms in Ex version, but in order for the device to work in explosive areas, you need to supply the PUE HX5.EX terminal. Use the potential High Capacity HRP Platforms work with one of two weighing terminals: PUE 7.1 or PUE HY10. The terminals&rsquo; software enables you to archive your work results, ensures data safety, and allows the platforms to work with powerbanks from our offer. In turn, you will select appropriate antivibration tables for the platforms, and thus increase the readability of your measurements.