Shimadzu Nexera Hybrid UHPLC System with Dual LC-20ADXR Pumps and SPD-40 UV-VIS Detector

The Shimadzu Nexera Hybrid UHPLC System is an integrated ultra-high performance liquid chromatography platform optimized for advanced analytical workflows in biotechnology, pharmaceutical, and clinical diagnostics. Equipped with dual LC-20ADXR binary gradient pumps, the system delivers precise high-pressure solvent mixing with flow rates from 0.0001 to 10 mL/min and maximum pressures up to 66 MPa, suitable for complex gradient elution and high-throughput bioprocessing applications.

Key components include the SPD-40 UV-VIS detector, featuring a D2 lamp light source spanning 190–700 nm wavelength range, an 8 nm bandwidth, and a sensitive 10 mm optical path flow cell, ensuring accurate molecular absorbance measurements essential for molecular diagnostics and gene editing research. The SIL-30ACMP autosampler supports automated sample injection from multiple microtiter plate formats (96/384 well, deep well) and vial racks with volumes up to 50 µL, enabling efficient, reproducible sample handling for synthetic biology and biomanufacturing studies.

The CTO-20A column oven provides uniform forced-air temperature control between ambient +10°C to 85°C with ±0.1°C precision, critical for stable chromatographic separations in protein characterization and impurity profiling. The DGU-20A5R degassing unit improves baseline stability by removing dissolved gases from solvents, enhancing method robustness in molecular cloning workflows.

System integration is managed via the CBM-20A controller, ensuring seamless communication across modules and software-based control. The complete UHPLC setup is validated for pharmaceutical drug analysis, environmental contaminant screening, food safety, and bioanalytical research, widely adopted in academic and industrial biotechnology laboratories.

Technical Highlights:

Widely used in biotechnology, this system is critical for molecular diagnostics, biopharmaceutical production pipelines, and synthetic biology research, providing high reproducibility and ruggedness for complex analytical separations. Its precise pump and detector performance support applications in live-cell imaging sample prep, fluorescence analysis workflows, and clinical bioanalysis. The comprehensive pre-owned package includes power conditioner, solvent trays, and communication cables, ready for immediate deployment with warranty and technical support.

This UHPLC system presents a cost-effective, sustainable choice for laboratories demanding reliable performance in gene editing, bioprocessing, and molecular cloning workflows, ensuring reproducible quantitation of biomolecules and impurities.