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Agilent 1290 Infinity I HPLC G4220A Binary Pump for High-Precision Liquid Chromatography
Product Description
The Agilent 1290 Infinity I HPLC G4220A Binary Pump is designed for high-pressure liquid chromatography applications requiring precise solvent delivery and gradient formation. This binary pump features a dual-piston in-series design with a servo-controlled variable stroke, enabling a highly accurate and stable flow rate range from 0.001 to 5.0 mL/min with fine 0.001 mL/min increments. The pump achieves a flow precision of ≤0.07% RSD and flow accuracy of ±1%, ensuring reliable performance for demanding analytical methods in molecular diagnostics, bioprocessing, and synthetic biology workflows.
Key features include:
- Seal wash system for extended pump seal lifetime during buffer applications
- Solvent selection valve providing flexibility for diverse chromatographic methods
- Jet Weaver mixing technology minimizing delay volume (<45 µL) for optimal solvent blending and gradient accuracy
- Automatic purge functionality enabling easy, unattended system preparation
- Fast defill function improving intake efficiency and delivery performance
- Two-channel integrated degassing unit with 1.5 mL internal volume per channel, enhancing flow stability and detector sensitivity
- Supports operating pressures up to 120 MPa (1200 bar) at 2 mL/min and 80 MPa (800 bar) at 5 mL/min
The pump’s compact footprint (9.3 × 13.5 × 17 inches) and robust construction (48 lbs) fit seamlessly into modern laboratories engaged in biomedical engineering, cell imaging, and biomanufacturing research. Its precision control and stable solvent delivery make it essential for workflows such as gene editing analysis, molecular cloning, and biopharmaceutical production pipelines requiring reproducible gradient elution and high-throughput analysis.
This binary pump is a critical component for analytical scientists and researchers focused on high-fidelity chromatographic separations, widely used across clinical diagnostics, synthetic biology research, and biotechnology sectors.
Item Stats
Product details
| Hydraulic System | Two dual-piston in-series pumps with proprietary servo-controlled variable stroke design, power transmission by ball screws, and smooth motion control of pistons for active damping |
| Settable Flow Range | 0.001 to 5.0 mL/min, in 0.001 mL/min increments |
| Flow Precision | ≤ 0.07 % RSD or 0.005 min SD, whatever is greater (0.2 to 5.0 mL/min) |
| Flow Accuracy | ± 1 % or 10 uL/min, whichever is greater. Pumping degassed H2O at 10 MPa (100 bar) |
| Pressure Operating Range | Up to 120 MPa (1200 bar) at up to 2 mL/min; 80 MPa (800 bar) at 5 mL/min |
| Pressure Pulsation | < 1 % amplitude or < 0.5 MPa (5 bar), whichever is greater |
| Compressibility Compensation | Automatic, pre-defined, based on mobile phase selection |
| Gradient Formation | High-pressure binary mixing |
| Delay Volume | Jet Weaver V35: <45 uL; Jet Weaver V100: <75 uL. For lowest delay volumes, the Jet Weaver can be removed |
| Settable Composition Range | Settable range: 0 to 100 %; 1 – 99 % or 5 ul/min per channel, whichever is greater |
| Composition Precision | < 0.15 % RSD or 0.01 min SD, whatever is greater |
| Composition Accuracy | ± 0.35 % absolute (5 to 95 %, 0.2 to 5.0 mL/min) |
| Solvent Selection Valve | Included as a standard part of the pump |
| Integrated Degassing Unit | Number of channels: 2; Internal volume per channel: 1.5 mL |
| Dimensions (H × W × D) | 9.3 x 13.5 x 17 in. (24 × 34.5 × 43.5 cm) |
| Weight | 48 lb. (21.8 kg) |
| Electrical Requirements | 100 to 240 V, 50/60 Hz, 90 VA |
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