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Agilent 1260 Infinity I HPLC System with Diode-Array and Fluorescence Detectors
Product Description
The Agilent 1260 Infinity I HPLC System combines precise flow control, detection, and temperature management for advanced chromatographic analyses:
- Wavelength Ranges:
- DAD: 190 to 640 nm
- FLD (Excitation & Emission): 200 to 1200 nm
- Flow Rate Range: 0.001 to 5 mL/min with pressure capability up to 600 bar
- Pump: High-pressure binary pump with integrated dual pumps for high-pressure mixing and rapid gradient formation
- Autosampler: Flow-through needle design minimizing sample carryover; high-speed and variable volume injection
- Temperature Control:
- ALS thermostat: 4°C to 40°C for sample temperature stability
- Column compartment: Thermostatted with Peltier elements ensuring high stability and reproducibility
- Detectors:
- DAD provides high sensitivity with programmable slit widths (1, 2, 4, 8, 16 nm) and fast data acquisition up to 80 Hz
- FLD features a high-intensity flash lamp, multi-wavelength spectral scanning, and simultaneous multi-signal detection
- Software: Includes Agilent ChemStation Workstation for instrument control, data acquisition, and analysis
- Solvent Handling: Four solvent access for isocratic or gradient analysis; degassing through four vacuum chambers and semipermeable tubing system
- Diagnostic Tests Performed:
- DAD & FLD: Filter, dark current, holmium oxide spectrum, lamp intensity, wavelength accuracy, baseline noise
- Pump: Pressure tightness (up to 400 bar), leak test
- Column compartment: Thermostat cooling/heating performance
- Autosampler: Injector sequence functionality
- System: 4 peak isocratic standard functionality test
The system is designed for flexible method development, high throughput workflows, and repeatable, high-resolution chromatographic separation. It is factory tested to ensure specifications compliance before shipment.
Item Stats
Product details
| DAD Detector | Specification |
| Detection Type | 1024-element photodiode array |
| Light Source | Deuterium and tungsten lamps |
| Wavelength Range | 190 - 640 nm |
| Short Term Noise (ASTM) Single and Multi-Wavelength | < ± 0.7 × 10^-5 AU at 254 nm and 750 nm |
| Drift | < 0.9 × 10^-3 AU/hr at 254 nm |
| Linear Absorbance Range | > 2.0 AU (5%) at 265 nm |
| Wavelength Accuracy | ± 1 nm |
| Wavelength Bunching | 1 - 400 nm |
| Slit Width | 1, 2, 4, 8, 16 nm / programmable slit |
| Diode Width | < 1 nm |
| Signal Data Rate | Up to 80 Hz |
| Spectra Data Rate | Up to 80 Hz |
| Flow Cell | Standard: 13 µL volume, 10 mm cell path length and 120 bar (1740 psi) pressure maximum |
| Control and Data Evaluation | Agilent ChemStation for LC |
| FLD Detector | Specification |
| Detection Type | Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis |
| Performance Specifications | Single wavelength operation: • RAMAN (H2O) > 500 (noise reference measured at signal) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell • RAMAN (H2O) > 3000 (noise reference measured at dark value) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell Dual wavelength operation: RAMAN (H2O) > 300 Ex 350 nm, Em 397 nm and Ex 350 nm, Em 450 nm, standard flow cell. |
| Light source | Xenon Flash Lamp, normal mode 20 W, economy mode 5 W, lifetime 4000 h |
| Pulse frequency | 296 Hz for single signal mode 74 Hz for economy mode |
| Excitation Monochromator | Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 300 nm |
| Emission Monochromator | Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 400 nm |
| Reference System | In-line excitation measurement |
| Timetable programing | up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters |
| Spectrum acquisition | Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200 – 400 nm, 10 nm step) Step size: 1 – 20 nm Spectra storage: All |
| Wavelength characteristic | Repeatability+/- 0.2 nm Accuracy+/- 3 nm setting |
| Flow cells | Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz |
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