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Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader
Product Description
The Molecular Devices SpectraMax M3 is a multi-mode microplate reader featuring:
- Wavelength range: 200 to 1000 nm
- Wavelength bandwidth: ≤ 4.0 nm full width half maximum
- Wavelength accuracy: ± 2.0 nm
- Detection modes: Absorbance (UV-Vis), fluorescence intensity, luminescence
- Dual monochromators allow precise wavelength selection with 1 nm increments
- Top-read and bottom-read modes for enhanced sensitivity in solution and cell-based assays
- PathCheck technology provides accurate pathlength correction for absorbance measurements
- Temperature control: ambient +2°C to 60°C with ±1°C accuracy
- Dedicated luminescence PMT for low background noise and maximum signal
- Compatible with 6-, 12-, 24-, 48-, 96-, and 384-well microplates, plus cuvette port for flexibility
- Robotics-compatible design supports automation in high-throughput workflows
- Xenon flash lamp for consistent, reliable illumination
- Built-in programmable shaker with timing from 0 to 999 seconds
- Supports endpoint, kinetic, spectrum, and area-well scanning read types
- Simultaneous multiwavelength read capability (up to four wavelength pairs) improves efficiency for FRET and multi-label assays
Applications include nucleic acid and protein quantitation, ELISAs, enzyme kinetics, cytotoxicity assays, drug dissolution testing, and various biochemical reporter assays. The system combines multi-detection capabilities with high optical performance similar to dedicated spectrofluorometers and spectrophotometers.
Item Stats
Product details
| Wavelength Selection | Monochromator, tunable in 1 nm increments |
| Wavelength Range | 200 to 1000 nm |
| Wavelength Bandwidth | ≤ 4.0 nm full width half maximum |
| Wavelength Accuracy | ± 2.0 nm across wavelength range |
| Wavelength Repeatability | ± 0.2 nm |
| Photometric Range | 0.000 to 4.000 OD |
| Photometric Resolution | 0.001 OD |
| Photometric Accuracy (Microplate) | < ± 0.006 OD ± 1%, 0 to 2 OD |
| Photometric Accuracy (Cuvette) | < ± 0.005 OD ± 1%, 0 to 2 OD |
| Photometric Precision | < ± 0.003 OD ± 1%, 0 to 2 OD |
| Baseline Flatness | < 0.001 OD |
| Stray Light | < 0.05% @ 230 nm |
| Dual Monochromators | 1 nm increment selection |
| EX Wavelength Range | 250 to 850 nm |
| EM Wavelength Range | 250 to 850 nm |
| Wavelength Bandwidth (EX, EM) | 9 nm |
| Top-Read Optimized Sensitivity | ≤ 1 pM (fluorescein) in 96 wells≤ 1.5 pM (fluorescein) in 384 wells |
| Top-Read Guaranteed Sensitivity | < 5 pM (fluorescein) in 96 wells or cuvette < 20 pM in 384 wells |
| Sensitivity | < 2 fg/well for firefly luciferase in 96- and 384-well microplates (top read) |
| Wavelength Range | 250 to 850 nm |
| Crosstalk | < 0.5% in 96- and 384- well microplates |
| Optimized Sensitivity | ≤ 43 pM ATP in 96 wells |
| Guaranteed Sensitivity | ≤ 75 pM ATP in 96 wells |
| Plate Formats | 6, 12, 24, 48, 96, 384 wells |
| Light Source | Xenon flash lamp (1 joule/flash) |
| Detectors | 2 Photomultiplier tubes (PMT) |
| Read Time | 96 wells: Abs 18 sec., Fl 17 sec., Lumi 120 sec. 384 wells: Abs 49 sec., Fl 48 sec., Lumi 420 sec. |
| Shaker Time | 0 to 999 seconds |
| Temperature Control | Ambient +2°C to 60°C |
| Temperature Uniformity | < 1°C at 37°C set point |
| Temperature Accuracy | ± 1°C at 37°C set point |
| Dimensions (H x W x D) | 21.8 x 51.2 x 39 cm (8.6 x 22.5 x 15.4 in) |
| Weight | 16.4 kg (36 lbs) |
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