Bio-Rad Gene Pulser Xcell Electroporation System

The Nucleofector technology is a major improvement of the well known electroporation technology. The (more) Nucleofector technology involves a combination of optimized electrical parameters and specific Nucleofector solutions for different cell types. This combination permits the crucials step: delivery of the DNA of interest straight into the nucleus. As the DNA quickly reaches the nucleus, expression can begin without delay. The nucleofected cells are thus ready for analysis after a short incubation time of only 2 – 8 hours. DNA quality: 1 – 5 µg plasmid concentration per nuclefection sample (100 µl), plasmid concentration can be increased up to 10 µg per sample. This may lead to higher gene transfer efficiencies, but at the same time may increase mortality of the cells. Cell density: 2 x 105 – 1 x 107 cells per nuclefection sample for suspensions cells 2 x 105 – 2 x 106 cells per sample for adherent cells Cell densities of < 2 x 105 cells per smaple are not advisable beacause the gene transfer efficiency may decrease and mortality may increase. Dimensions (WxDxH): 300 x 230 x x110 mm Weight: 2.5 kg

The Gemini X2 Systems is designed for researchers who need the ultimate experiment flexibility. In o (more)ne easy setup, square wave and exponential decay waves can be applied to cells in any format. These systems incorporate remote operation functionality via footswitch or PC and internal log storage of experiment data for easy optimization, quality control requirements and troubleshooting. Electroporation of suspension cells can be achieved in 96-well plates with the HT plate handler. Usable with all cell electroporation Features: Square Waveform, Multi-Pulsing Square Wave, Exponential Decay Waveform, Multi-Pulsing Exponential Decay, Resistance/Pulse Monitoring, Experiment Log Storage, Preprogrammed Protocols, Unlimited Custom Protocol Storage, Remote Operation, PC Communications Voltage Range: 5 to 3000 V Pulse Width Range: 10 μs to 5 s Pulse Interval: 100 ms to 30 s Dimensions (DxWxH): 324 x 286 x 216 mm Weight: 7.3 kg

The offered instrument / system is fully functional tested and ready to use. FURTHER DETAILS TO FOLL (more)OW

The Gene Pulser Xcell System is a flexible, modular electroporation system for transfecting every ce (more)ll type from primary, suspension, and difficult-to-transfect cells, including T cells, to bacteria and fungi. The system is composed of a main unit, two accessory modules, the capacitance extender (CE module) and the pulse controller (PC module), and a ShockPod cuvette chamber. Features and Benefits Universal electroporation — transfects all cell types, from primary, stem, and T cells to bacteria and fungi Flexibility with your experiment parameters for the highest transfection efficiency and viability Preset protocols — ready-to-use preoptimized protocols for frequently used mammalian and bacterial cell types Manual operation — allows for entry and storage of new parameters or editing of all parameters Optimization protocols — enables the best conditions to be determined using incremental voltage steps Exponential and square waveforms — allows for optimal delivery Confidence in your results — uses PulseTrac circuitry and arc-protection features to ensure sample protection and reproducible results Protocol library — collection of electroporation protocols for every cell type including primary, immortal, and bacterial cells Prevalidated published protocols — saves time optimizing conditions Easily accessed data management — storage and recall of parameters and results from previous 100 experiments, useful for troubleshooting Output Waveform: Exponential or square Output Voltage: 10 – 3,000 V Capacitance: 10 – 500 V, 25 – 3,275 µF in 25 µF increments, 500 – 3,000 V, 10, 25, 50 µF Resistance (parallel): 50 – 1,000 Ω in 50 Ω increments, plus infinity Sample resistance: 20 Ω minimum at 10 – 2,500 V, 600 Ω minimum at 2,500 – 3,000 V Square-wave timing: 10 – 500 V: 0.05 – 10 ms in 0.05 ms increments, 10 – 100 ms pulse in 1 ms increments, 1 – 10 pulses, 0.1 – 10 sec interval/ 500 – 3,000 V: 0.05 – 5 ms in 0.05 ms increments, 1 – 2 pulses, 5 sec minimum interval Dimensions (WxDxH): 310 x 300 x 280 mm Weight: 11.6 kg

Foster + Freeman MVC3000 Fuming Cabinet This item was removed from a university lab where it was sur (more)plus to requirements. The cabinet powers on and is in reasonable cosmetic condition. Upon powering up, the cabinet initiates a 5-minute Mini Purge, after which the electronic door can be opened. However, the system displays a 'Filter Fault' status after startup, indicating a missing filter.Therefore, further testing could not be conducted. There is no filter fitted, the unit should work fine with one in. Specifications:- 1 Shelf: 74 x 58 cm - 2 Baskets: 57 x 30 x 15 cm - Underneath space: 75 x 64 x 32 cm Model: MVC3000Serial No: 3175

Perkin Elmer Spectrometer LS 50 B This item was removed from a university laboratory where it was s (more)urplus to requirements. It powers on, but then started emitting a bad smell, suggesting a possible issue with the power supply. Therefore, it is listed as Spares/Repair.

IKA R 1302 Dissolver Stirrer Radial flow, for drawing the material to be mixed from the top and the (more) bottom. High turbulence, high shearing forces. Particle reduction. Used at medium to high speeds.Stirrer Ø: 100 mmShaft Ø: 10 mmShaft length: 350 mm INCLUDED: The IKA R 1302 Dissolver Stirrer includes: - Operator's Guide - Manufacturer's Documentation - 30 Day Full Parts and Labor Warranty Request a Formal Quote

Ohaus AX4202 Adventurer Toploading BalanceStriking theideal balance between inventive features and f (more)unctional, uncomplicated weighingcapabilities, the Ohaus Adventurer Pro II AX4202 incorporates all of theapplications necessary for routine weighing and measurement activities. With acolor touchscreen, GLP/GMP compliance capabilities, two USB ports, and muchmore, Adventurer AX4202 is the most complete balance in its class. 4200 g capacity, 0.01 g readability 175 x 195 mm platform Internal calibration 4.3 inch Full-color VGA graphic touchscreen with user-controlled brightness RS232, USB Host, USB Device (included). GLP/GMP data output with real-time clock Selectable environmental filters, auto tare, user selectable span calibration points, software lockout and reset menu User selectable communication settings and data print options User definable project and user IDs Up to 9 operating languages Click Here to View Product Video INCLUDED: The Ohaus AX4202 Adventurer Toploading Balance includes: - Power Cord - Operator's Guide - Manufacturer's Documentation - 2 Year Manufacturer's Warranty

Ohaus SHRK07AL2 Rocking Shaker, 2 TierThe OhausSHRK07AL2 Analog Tier 2 Rocking Shaker is an easy and (more) economical option for allof your rocking needs. The Rocking Shaker is ideal for cell culture andblotting applications and is designed to be used in a variety of environmentalconditions. Rocker is supplied with two 14 x 11" (35.6 x 27.9 cm) non-skidrubber mats.The SHRK07AL2is designed for use in a range of lab applications in a variety ofenvironmental conditions. Rocking Shakers provide a seesaw-like motion, whileWaving Shakers offer a smooth, low-foaming, 3D wave motion for precise speedcontrol. All models include a non-slip mat, and most models are available witha stacking tray option.Microprocessorcontrol allows for independent control knobs for tilt, speed and time (analogmodels).Timerdisplays elapsed time or when programmed to a set time, will turn off when timereaches zero.Speed rampingfeature slowly increases speed to desired setpoint to avoid splashing.Audible/visual signals activate when the system detects an obstruction or trayoverload. INCLUDED: The Ohaus SHRK07AL2 Rocking Shaker, 2 Tier includes: - Non-Skid Rubber Mat - Tier Kit - Power Cord - Operator's Guide - Manufacturer's Documentation - 2 Year Manufacturer's Warranty

REFRIGERATED LABORATORY INCUBATORS ST SMART POL-EKO ST - STABILITY AND QUALITY AT THE BEST PRICE Bei (more)ng able to both heat and cool, ST incubators are perfect for the stable and safe incubation of samples, in any working environment. The ST series maintains a constant temperature from 3°C to 40°C with excellent precision. As an option, the range can be extended up to +70 °C. Operation at ambient temperature +10°C...+28°C, for cooled incubators with glass door +10°C...+25°C. It's necessary to purchase automatic defrosting function (ST/CHL PLUS) for all units operating at set point <=5°C to prevent icing. Large touch display, LED lighting, alarms, lock, simple and comfortable design, will make using these incubators a breeze; and thanks to the programming with time and temperature profiles, you will be able to carry out highly precise and repeatable incubations. But it doesn't end here, continue reading and discover all the other surprising features of these incubators! WHICH VERSION? THE THREE VERSIONS OF THE ST MODEL The three models differ in the materials of the internal chamber, the materials of the shelves and the safety class. This subdivision allows you to choose based on your real needs, meeting your budget. BASIC: aluminum interior; metal grid shelves; safety class 1.0 (DIN 12880). COMFORT: stainless steel interior according to DIN 1.4016; stainless steel grill shelves; safety class 1.0. PREMIUM: Interior in acid-resistant stainless steel according to DIN 1.4301; stainless steel grill shelves; safety class 2.0. As an option, at the bottom of the page, you can find the ST-INOX accessory; this allows you to order your ST refrigerated incubator with polished stainless steel exteriors, rather than powder-coated sheet metal (light gray with graphite front panel) as standard. ABSOLUTE CONTROL CONTROL PANELS The SMART control panel is among the most complete you can find on the market. Large symbols and temperature values ​​guarantee comfortable and clear reading, combined with disarming ease of use. The latest generation touch screen responds to commands even if touched with latex gloves and is equipped with a large display: all values ​​are perfectly readable from any angle, regardless of the intensity of the light. More and more? POL-EKO has already thought about it. For those who want cutting-edge functions and the freedom of transfer and remote use, POL-EKO offers you the SMART PRO controller. You can find all the differences between the SMART and SMART PRO panel in our dedicated post: insights on SMART and SMART PRO. With the SMART PRO versions, more accessories and options will also be available. DO YOU KNOW THE ADVANTAGES OF AN ADVANCED CONTROLLER LIKE THE SMART PANEL? - Sharp 4.3' color touch screen - Quick and easy data transfer: via LAN or USB - Fast, multi-segment, time and temperature programming - Visual and audible alarms with colored bar on display - Internal memory for programs and data storage - User manual downloadable directly from the controller - Loop function up to 255 times or infinite - Controls can be managed even when wearing latex gloves - Recording of the minimum, average and maximum temperature value for each segment - Overview of the set and current parameters during operation - Operation in temperature or time priority mode - ... and much more QUALITY FIRST Resistant. Durable. POL-EKO incubators are designed and built to guarantee the best performance. They are a smart purchase, for customers who want to invest in the future of their laboratory. By choosing these products you will have precise results for years and years, repeatable and in complete comfort. The ST SMART series incubators are built with the best materials and equipped with the most important accessories, to give you a complete and ready-to-use standard product. - Insulated door with lock - Internal LED light - Optional internal and/or external glass door - USB port for downloading recording data, events, manuals, service. - LAN port - Open door alarm - Access port for external sensor on the side - Quality control protocol (in +37°C) - Available with FOT: phytoperiodic system – day and night simulation. To find out more, download our informative PDF: The photoperiodic (FOT) and phytotron (FIT) systems by POL-EKO. The three versions of the ST models The three models differ in the materials of the internal chamber, the materials of the shelves and the safety class. This subdivision allows you to choose based on your real needs, meeting your budget. BASIC: aluminum interior; wire shelves; over temperature protection class 1.0. COMFORT: stainless steel interior according to DIN 1.4016; stainless steel wire shelves; over temperature protection class 1.0. PREMIUM: Interior in acid-resistant stainless steel according to DIN 1.4301; stainless steel wire shelves; over temperature protection class 2.0. As an option, at the bottom of the page, you can find the ST-INOX accessory; this allows you to order your ST refrigerated incubator with polished stainless steel exteriors, rather than powder-coated sheet metal (light gray with graphite front panel) as standard. ST SMART SERIES POL-EKO offers 10 models of refrigerated incubators with capacities from 70 to 1540 litres. The ST series also offers multi-chamber models with two or three separate and independently controlled internal compartments. These solutions allow you to save space in the laboratory and perform tests at different temperatures. Mixed multichambers are also available, such as incubator-refrigerator or incubator-freezer combinations. DETAILS AND ACCESSORIES Every detail of your POL-EKO models is designed to make routine work as simple and comfortable as possible. Below, the front and rear view. 1 - rating plate 2 - Temperature sensor 3 - Shelf 4 - Access port for external sensor 5 - Adjustable feet 6 - Solid external door 7 - Ventilation 8 - Lock 9 - Touch screen control panel 10 - USB port 11 - Door open sensor 1 - Main switch 2 - Condenser 3 - Cooling system 4 - Fuse 5 - Main power socket 6 - LAN port For larger models: a - Anchor rods b - Integrated power cable c - Fuse d - Main switch e - LAN port OPTIONS POL-EKO allows you to increase the possibilities of your incubator with quality accessories; to better adapt to particular applications or to equip the instrument with safety systems and advanced connections. In video, the animation shows the ST2 refrigerated incubator with the possible shelves available: shelf with compartments; perforated shelf; grill shelf. - Internal glass door - External glass door - Grid shelf - Stainless steel grill shelf - Perforated shelf - Reinforced shelf - Stainless steel basins - Aluminum or stainless steel drawer with guides - Wheels - Coffee table with wheels - Automatic defrost function - Heating up to 70°C - Photoperiodic system - POT - Internal socket - LabDesk software - IQ OQ PQ calibration and qualification - Overtemperature protection class 3.3 according to DIN 12880 - Backup battery for the display in case of power failure - Dry alarm contact - LAN cable - Non-standard 20/60/100 mm access door - Additional 30 mm access door