Amaxa Nucleofector 1 Electroporation System

The Nucleofector technology is a major improvement of the well known electroporation technology. The (more) Nucleofector technology involves a combination of optimized electrical parameters and specific Nucleofector solutions for different cell types. This combination permits the crucials step: delivery of the DNA of interest straight into the nucleus. As the DNA quickly reaches the nucleus, expression can begin without delay. The nucleofected cells are thus ready for analysis after a short incubation time of only 2 – 8 hours. DNA quality: 1 – 5 µg plasmid concentration per nuclefection sample (100 µl), plasmid concentration can be increased up to 10 µg per sample. This may lead to higher gene transfer efficiencies, but at the same time may increase mortality of the cells. Cell density: 2 x 105 – 1 x 107 cells per nuclefection sample for suspensions cells 2 x 105 – 2 x 106 cells per sample for adherent cells Cell densities of < 2 x 105 cells per smaple are not advisable beacause the gene transfer efficiency may decrease and mortality may increase. Dimensions (WxDxH): 300 x 230 x x110 mm Weight: 2.5 kg

The BTX HT-200 plate handler allows for the Highthroughput System HT 25 or HT 96 for advanced molecu (more)lar delivery. The HT 96 (96-well) plates or the HT 25 (25-well) plates can be electroporated using a BTX generator (models: ECM 830 and ECM 630) with a plate handler. The Model HT-200 automatically switches columns in a Multi-well plate during electroporation using the Auto-track sensing featute. The Auto-track sensing allows rapid electroporation of an entire plate by setting up all columns and pressing one button. Voltage Range: 0 to 3000V DC Pulse Length Range: 10 µsec to 10 sec Pulse Number Range: 1 to 99 Dimensions (WxDxH): 230 x 215 x 140 mm Weight: 4.8 kg Including: 1x 96-Well Disposable Electroporation Plates, 4 mm, 250 µl, pkg. of 1 1x 96-Well Disposable Electroporation Plates, 2 mm, 125 µl, pkg. of 1 1x HT 25-Well Adapter for Plate Handler

The Gemini X2 Systems is designed for researchers who need the ultimate experiment flexibility. In o (more)ne easy setup, square wave and exponential decay waves can be applied to cells in any format. These systems incorporate remote operation functionality via footswitch or PC and internal log storage of experiment data for easy optimization, quality control requirements and troubleshooting. Electroporation of suspension cells can be achieved in 96-well plates with the HT plate handler. Usable with all cell electroporation Features: Square Waveform, Multi-Pulsing Square Wave, Exponential Decay Waveform, Multi-Pulsing Exponential Decay, Resistance/Pulse Monitoring, Experiment Log Storage, Preprogrammed Protocols, Unlimited Custom Protocol Storage, Remote Operation, PC Communications Voltage Range: 5 to 3000 V Pulse Width Range: 10 μs to 5 s Pulse Interval: 100 ms to 30 s Dimensions (DxWxH): 324 x 286 x 216 mm Weight: 7.3 kg

This BTX Electro Square Porator ECM 830 was removed from a university lab where it was surplus to re (more)quirements. The item powers on and is in reasonably good cosmetic condition, we are unable to test it further at our facility.

Electroporator Lonza 4D-Nucleofactor System. Formed by two modules: unit Core (control unit) and un (more)it-X unit-transfection). Unit X performs 16-to 32-transfections per minute. Includes instruction manual and original packaging. 2014. Specifications: 240 V; 50-60 Hz The Core Unit - Controlling the 4D-Nucleofactor™ System The X unit – Supporting Nucleofection™ of various cell numbers in different formats

Additional technical features: - External structure in steel painted with antiacid epoxy powder - In (more)ner chamber in stainless steel - 45 mm hole on the rear side for flue gas discharge with device for opening / closing the passage of cables, probes, etc. - 10 mm hole on the upper side for inserting the thermometer or probes - Temperature control with microprocessor - Adjustable safety thermostat - Safety class 3.1 - 4 work programs of 8 segments - Repeatability With the optional software you can control the operation of the programs in a very simple way and have all the data under control. Specifically, you can: - Create charts - Archive the history - Check and see all processes on a graph - Optimize times Quality materials: - External structure in steel sheet painted with anti-acid epoxy powder - Inner chamber in 18/8 stainless steel

This Agilent G3100A 3100 OFFGEL Fractionator System was removed from a university lab where it was s (more)urplus to requirements. The unit powers up and is in excellent cosmetic condition. The screen is in good condition and is clear. Item also comes with Quick Start Guide and a declaration of conformity certificate.

Thermo Scientific Multidrop 384 Microplate Dispenser Labsystems 832 Lab This was removed from a univ (more)ersity lab where it was surplus to requirements. The unit powers up with no faults, unable to test it further at our facility. It is in good cosmetic condition with minor marks.

IKA MultiDrive control MT Package Speed Range 3000 - 20000 rpm Motor principle Brushless DC Weighing (more) range 5 - 4000g Features - Versatile crushing capabilities for hard, soft, or fibrous samples with a range of vessels for coarse and fine crushing - MultiDrive control unit also mixes, grinds, and includes a dispersion vessel and a disposable tube for diverse applications - Features a USB interface for convenient actuation and documentation - High performance with variable rotational speeds from 3000 rpm to 20,000 rpm and a powerful 1000 Watt output - Interval operation mode enhances coarse crushing and thorough blending with simple activation - Integrated cooling in the milling cup for indirect heat dissipation, keeping coolant and sample separate - User-friendly operation ensured by a clear TFT-display Crushing without cross-contamination - this is possible with the MultiDrive control package including autoclavable disposable tubes BT 250 and the required TC 1 protective housing for dry samples. BT 250 is a disposable tube with a cross-shaped stainless steel beater and flow breakers. The plastic blending tube can be autoclaved. This effectively prevents cross-contamination even if it is used multiple times. An integrated RFID chip simultaneously stores and limits the lifespan of the blending tube. 5 spare seals are included in the scope of delivery. By replacing the seal, the service life of the blending tube can be extended. The disposable tube can only be operated with the TC 1 protective housing which is also included in the scope of delivery. MultiDrive universal crusher can perform a wide range of crushing tasks involving coarse and fine crushing, thanks to the variety of vessels available. There is a USB interface available for easy actuation and documentation respectively. High performance is guaranteed by a combination of variable rotational speeds, ranging from 3000 rpm to 20 000 rpm, and a 1000 Watt output. Interval programming is simple to activate at the press of a button. Interval operation is an asset during the coarse crushing of hard samples or for extra thorough blending. Integrated cooling in the milling cup, which allows indirect heat dissipation. Thus, coolant and sample remain separate. TFT-display guarantees user-friendly operation.Temperature measurement and vessel recognition using RFID. A temperature limit can be set for temperature-sensitive materials, or for specific planned reactions to protect against overheating. Weighing function is also integrated into the user-friendly workflow of the IKA MultiDrive control. Weighing is performed before crushing using the same mixing cup, so that no transfer of contents is required.INCLUDED: The IKA MultiDrive control MT Package includes: - 5 Spare Seals - IKA MultiDrive control - MultiDrive TC 1 Protective housing - MultiDrive MT 150.5 Milling tube, autoclavable - Manufacturer's Documentation - 2 Year Manufacturer's Warranty