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BioTek 405 LS Automated Microplate Washer for 96- and 384-Well Plates
Product Description
The BioTek 405 LS microplate washer is a precision instrument designed for sensitive and high-throughput washing of 96- and 384-well microplates, widely used in molecular diagnostics, bioprocessing, and immunoassays like ELISA. Available in two configurations—405LSR for 96-well plates and 405LSUSQ for 96- and 384-well plates with enhanced features—the system delivers consistent and repeatable wash cycles critical for biotechnology workflows and cell-based assays.
- Plate compatibility: Supports low profile and standard 96- and 384-well plates, including solid and filter bottom plates with pore sizes ranging from 0.45 to 1.2 µm, essential for filtration assays and bead-based washing.
- Manifold design: The 405LSR features a single 96-tube manifold, while the 405LSUSQ is equipped with a dual-action manifold comprising two independent 96-tube sets (192 tubes) allowing simultaneous dispense and aspirate operations, reducing cross-contamination risk and optimizing wash efficiency for biotechnological applications.
- Volume and precision: Dispense volumes range from 25 to 3,000 µL per well with 1 µL increments; achieving <3% CV at 300 µL for 96-well plates and <4% CV at 80 µL for 384-well plates ensures precise reagent delivery critical for molecular cloning and synthetic biology protocols.
- Wash cycle capacity: Programmable up to 250 cycles with adjustable flow rates and shaking (slow, medium, fast, or variable) enhances flexibility for delicate live-cell washing or rigorous bead washing in bioprocessing pipelines.
- Ultrasonic cleaning & clog detection: The 405LSUSQ model includes ultrasonic manifold cleaning and clog detection, reducing downtime and enhancing reliability during prolonged molecular diagnostic workflows.
- Software & integration: Onboard protocol creation and editing support standalone operation, while compatibility with BioStack automation systems and 21 CFR Part 11-compliant PC control meets regulatory compliance demands for clinical diagnostics and biomanufacturing environments.
- Physical specifications: Dimensions 17 × 29 × 15 inches, weight 40 lbs, and voltage input range of 100–240 VAC at 50/60 Hz make it adaptable for various laboratory settings globally.
This microplate washer is critical for applications demanding reliability and reproducibility in biotechnology research, including gene editing assays, synthetic biology, and biopharmaceutical production pipelines, by ensuring standardized plate preparation with minimal operator variability.
Item Stats
Product details
| Microplate types | 96-well, Low Profile and Standard, Solid and Filter Bottom (Pore Sizes 0.45 to 1.2 μm) Plates (405LSR) 96- and 384-well, Low Profile and Standard, Solid and Filter Bottom (Pore Sizes 0.45 to 1.2 μm) Plates (405LSUSQ) |
| Onboard software | Create, edit or run multiple protocols |
| Shaking | Programmable up to 60 minutes Slow, medium, fast or variable |
| Soaking | Programmable up to 60 minutes |
| Manifold type | 96 Tube Manifold straight dispenser (405LSR) Dual-Action wash 96- and 384-well plates. It has two sets of 96 dispense and aspirate tubes arranged in two manifolds that move independently of each other and support overflow wash and overfill protection for the most demanding assays. (405LSUSQ) |
| Volume range | 50 to 3,000 µL/well, in 1 µL increments (405LSR) 25 to 3,000 µL/well, in 1 µL increments (405LSUSQ) |
| Wash cycles | 1 – 250 |
| Dispense precision | <3% CV: 300 μL/well (96-well washing); <4% CV: 80 μL/well (384-well washing) |
| Residual volume | <2 μL/well (96- & 384-well plates), 96-tube manifold for 96 wells, 192-tube for 384 wells |
| Wash speed | 96-wells: 300 μL/well, 3 cycles: <30 s; 384-wells: 100 μL/well, 3 cycles: <80 s; 384-wells: 400 μL/well, 1 cycle: <20 s |
| Flow rates | High flow to low flow |
| Sterilization | Chemical |
| Power | 100 – 240 Volts AC. 50/60 Hz |
| Dimensions | 17x29x15″ |
| Weight | 40 lbs |
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