BD FACSCanto II Flow Cytometer

The BD FACSCanto II is an intuitive benchtop flow cytometer renowned for its precise and reliable performance. Capable of using two or three lasers to detect up to eight colors, it contains a true fixed alignment flow cell, minimizing startup times and enhancing reproducibility. Featuring an optimized optical system, the cytometer significantly boosts signal detection and resolution across multicolor assays, ideal for high-throughput clinical laboratories. The high automation level and quality control reduce both technician involvement and costs associated with repeated testing.

Lasers include a 405-nm solid state diode with 30-mW fiber output, a 488-nm solid state with 20-mW output, and a 633-nm HeNe with 17-mW output. The spatially separated beams reduce operator alignment needs with a fixed alignment procedure. Its optical-gel coupled 1.2 NA lens supports superior signal collection, while fluorescence is detected using 8 PMTs in a 4-2-2 configuration, enabling multicolor assay precision.

The FACSCanto II can handle fluorescence detection with a sensitivity of less than 100 MESF for FITC and less than 50 MESF for PE, processing particles up to 50 μm. It supports a sample flow rate of 10 μL/min to 120 μL/min, with an acquisition rate of 10,000 events per second. Its compact design, 145 kg weight, and tailored setup make it an ideal choice for modern laboratories.