Advion Interchim PuriFlash 5.250 Preparative Flash Chromatography System with UV Detection and Fraction Collector

The Advion Interchim PuriFlash 5.250 Preparative Flash Chromatography System is a robust platform designed for high-capacity preparative-scale purification, widely used in organic synthesis, medicinal chemistry, and biotechnology research. Its precise gradient pump system supports flow rates ranging from 1 to 250 mL/min with high linearity (<1) and low repeatability (<0.25), facilitating scalable and reproducible separation workflows critical in bioprocessing and synthetic biology applications.

Key components include an integrated UV-Visible detector operating over 200–400 nm wavelength range using diode array technology, allowing real-time monitoring of elution profiles essential for molecular diagnostics and biomanufacturing pipelines. The system features a quaternary gradient valve enabling flexible solvent mixing, an automated fraction collector accommodating up to 3 racks (44 tubes × 25 mL each), and supports both liquid and automated solid injections via a 10-port electric valve, enhancing workflow automation for gene editing reagent purification and metabolic profiling.

The PuriFlash 5.250 is equipped with dry-load and column holders, as well as a solvent tray with drainage system, ensuring safe and efficient operation. Its maximum operational pressure is 250 bar (3625 psi), allowing use with large column formats typical in pilot-scale separations. Power requirements range from 110 to 240 VAC with a maximum power consumption of 1000 Watts; total system weight is 54 kg distributed across control unit, pump, and collector modules.

This system is essential for laboratories involved in preparative purification of synthetic organic molecules, scale-up in process chemistry, and isolation of target fractions from complex mixtures. It is especially valued in biotechnology fields for molecular cloning workflows, synthetic biology research, and biopharmaceutical production pipelines demanding scalable, reproducible, and automated purification solutions.

Features and Benefits:

The PuriFlash 5.250 system is a critical asset for research teams conducting biotechnology-driven purification tasks, notably in gene editing reagent purification, molecular diagnostics, and synthetic biology research requiring precise fractionation of complex mixtures through preparative flash chromatography. Its flexibility, automation, and reliable performance make it a widely adopted instrument in biotechnology laboratories and pharmaceutical process development environments.