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Thermo Scientific NanoDrop One Microvolume UV-Vis Spectrophotometer for DNA, RNA, Protein Quantification
Product Description
The Thermo Scientific NanoDrop One is a precision microvolume UV-Vis spectrophotometer designed for rapid quantification of nucleic acids and proteins with sample volumes as low as 1 µL. It utilizes a Xenon flash lamp and a 2048-element CMOS linear image sensor to deliver accurate absorption spectra across a wavelength range of 190–850 nm with ±1 nm accuracy. Automated path length adjustment from 0.030 to 1.0 mm enables measurement of highly concentrated samples without dilution, benefiting workflows in molecular diagnostics, gene editing research, and bioprocessing.
Key technical features include:
- Quantification of dsDNA concentrations up to 27,500 ng/µL (pedestal) and BSA proteins up to 820 mg/mL
- Wide dynamic detection from 2.0 ng/µL to 27,500 ng/µL DNA and 0.06 to 820 mg/mL proteins using pedestal method
- Rapid measurement and data processing within 8 seconds, facilitating high-throughput labs focused on synthetic biology and biomanufacturing
- Integrated touchscreen interface for direct data review and export without requiring external software, improving workflow efficiency for biotechnology applications
- Connectivity options include USB-A ports, Ethernet, Bluetooth™, and Wi-Fi, supporting modern laboratory data management and integration
- Exceptional repeatability with a typical standard deviation of 0.002 A at 1.0 mm path length or 1% CV
The NanoDrop One is essential for researchers performing routine nucleic acid and protein quantification, OD600 measurements for microbial growth, and general UV-Vis absorbance assays in fields such as biomedical engineering, clinical diagnostics, live-cell imaging workflows, and biomanufacturing quality control. It is particularly advantageous in molecular cloning workflows where minimal sample consumption and rapid, accurate data are critical.
Its pedestal-only design suits laboratories prioritizing fast, stand-alone quantification, while labs requiring cuvette-based or kinetic measurements may select the related NanoDrop OneC model. This spectrophotometer’s robustness and precision make it a trusted instrument widely adopted across biotechnology and life sciences research sectors.
Item Stats
Product details
| Lamp | Xenon flash lamp |
| Wavelength Accuracy | ±1 nm |
| Wavelength Range | 190–850 nm |
| Concentration | Maximum, dsDNA: Pedestal: 27,500 ng/µL , BSA (IgG): Pedestal: 820 (400) mg/mL |
| Connections | Three USB-A ports, Ethernet, Bluetooth™ and Wi-Fi |
| Detection Range | dsDNA: Pedestal: 2.0 ng/µL, Cuvette: 0.2 ng/μL BSA (IgG): Pedestal: 0.06 (0.03) mg/mL, Cuvette: 0.006 (0.003) mg/mL |
| Detector Type | 2048-element CMOS linear image sensor |
| Measurement Time | Measurement and Data Processing Time 8 seconds |
| Pathlength (Metric) | 0.030 to 1.0 mm auto-ranging |
| Photometric Accuracy Instrument | 3% at 0.97 A, 302 nm, (Absorbance expressed at Abs/mm at 25°C) |
| Photometric Range | 10 mm equivalent: Pedestal: 0–550 A Cuvette: 0–1.5 A |
| Repeatability | Measurement repeatability: Typical: 0.002 A (1.0 mm path) or 1%CV, whichever is greater (SD of 10 individual measurements at 0.97 A) |
| Sample Volume (Metric) | Minimum 1µL |
| Spectral Bandwidth | 1.8 nm (FWHM at Hg 254 nm) |
| Dimensions (L x W x H) | 17x12x17″ |
| Weight | 15 lbs |
| Power | Operating: 12–18 W, Standby: 5 W, 12V (DC) |
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