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Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader with UV-Vis and Fluorescence Detection (200–1000 nm)

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Product Description

The Molecular Devices SpectraMax M5 is a versatile multi-mode microplate reader designed for advanced laboratory applications including molecular diagnostics, bioprocessing, and synthetic biology research. It supports a wide range of detection modes:

  • Absorbance (200–1000 nm) using a xenon flash lamp and silicon photodiode, ideal for DNA/RNA quantitation and protein assays
  • Fluorescence intensity (250–850 nm) from both top and bottom for diverse assay formats, including live-cell imaging and fluorescence analysis
  • Luminescence (250–850 nm) sensitive enough for reporter assays and bioluminescence-based gene editing studies
  • Time-resolved fluorescence (TRF) and fluorescence polarization (FP) for enhanced signal discrimination in high-throughput screening and drug discovery workflows
  • Homogeneous time-resolved fluorescence (HTRF) assays widely used in molecular biology and clinical diagnostics

Key technical features include dual scanning monochromators with 1 nm spectral resolution, enabling precise wavelength selection and optimization for multiplex and ratiometric assays across 6 to 384 well plates. The PathCheck sensor normalizes absorbance measurements to a 1 cm pathlength in microplates, enhancing data reliability without standard curves.

The instrument incorporates:

  • Auto PMT optimization for extended fluorescence dynamic range by per-well gain adjustment
  • Top and bottom optical reading modes to accommodate various assay setups
  • A cuvette port for standalone UV-Vis measurements, broadening experimental flexibility
  • Temperature control from ambient +2°C up to 60°C, supporting kinetic and enzyme assays important in molecular cloning workflows
  • Linear plate shaking to improve assay uniformity during incubation
  • Integration capabilities with the StakMax microplate stacker for walk-away automation in high-throughput biomanufacturing and synthetic biology pipelines
  • Compatibility with SoftMax Pro and SoftMax Pro GxP software, ideal for regulated environments and facilitating data acquisition, analysis, and compliance

Typical sensitivity specifications include fluorescence detection down to 1 fmol/well FITC in 96-well plates, less than 2 fg/well firefly luciferase luminescence, and precise fluorescence polarization measurements. The system supports endpoint, kinetic, spectral scan, and well scan read types, enabling flexibility across biotechnology and biomedical engineering research domains.

At 20.14 kg, with dimensions supportive of standard lab workflows, the SpectraMax M5 is a robust instrument engineered and manufactured in the USA, suitable for a wide array of research fields including cell viability, signaling studies, ELISA development, and molecular cloning assays.

This multi-mode microplate reader is critical in biopharmaceutical production pipelines and molecular biology research, where sensitive, precise, and flexible detection methods are paramount.


Item Stats

Status
For Sale
State
used
Brand
Molecular BioProducts
Model
Spectramax M5
Location
United States

Product details

makeMolecular BioProducts
hertz50Hz/60Hz
modelSpectramax M5
serialMV06027
weight37.0 Lb
voltage120V/220V
includesPower cord included |
warranty30-Day Warranty On Part
dimensions23.0in x 19.0in x 6.0in
shipping_typeFedEx Ground
item_conditionGood
manufacturing_dateDoes Not Apply

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