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BioTek Synergy H1m Monochromator-Based Multi-Detection Microplate Reader
Product Description
The BioTek Synergy H1m is a versatile monochromator-based microplate reader designed for multiplexed biochemical and cell-based assays in biotechnology and molecular diagnostics. Optimized for fluorescence intensity detection with continuously variable monochromators, it allows excitation and emission wavelength adjustments in 1 nm increments across 250–700 nm, enabling precise fluorescence measurements crucial for synthetic biology and bioprocessing workflows.
Key features include:
- Detection modes: UV-Vis absorbance (230–999 nm, 1 nm increments), fluorescence intensity (top and bottom), and luminescence
- Light sources: Xenon flash lamps for absorbance and fluorescence excitation, enhancing sensitivity across a wide range of assay formats
- Sensitivity: Detection limits as low as 20 pM for sodium fluorescein, enabling detection in molecular cloning workflows and gene editing research
- Microplate compatibility: Supports 6- to 384-well plates, Petri dishes, cell culture dishes, and Take3 micro-volume plates, enhancing throughput for biomanufacturing and cell imaging applications
- Environmental control: Four-zone incubation up to 45 °C with condensation control and multiple shaking modes (linear, orbital, double orbital), critical for live-cell assays and long-term kinetic studies
- Software control: Gen5 software for method setup, data acquisition, and flexible analysis, aiding biomedical engineering and molecular diagnostics laboratories
With rapid reading speeds (e.g., 22 seconds for 384 wells) and pathlength correction, the Synergy H1m supports high-throughput biotechnology research with precise wavelength tuning, making it widely adopted in fluorescence analysis, live-cell imaging, and biopharmaceutical production pipelines. The monochromator design offers method adaptability beyond fixed-filter readers, ensuring compatibility across diverse clinical diagnostics and synthetic biology workflows.
Item Stats
Product details
| Detection Modes | UV-Vis absorbance Fluorescence intensity (top and bottom) Luminescence |
| Microplate Types and Lab Glassware | 6- to 384-well plates, Petri and cell culture dishes Take3 Micro-Volume Plates |
| Light source | Xenon Flash |
| Detector | Photodiode |
| Wavelength Selection | Monochromator |
| Wavelength range | 230 – 999 nm, 1 nm increment |
| Bandwidth | <4 nm (230-285 nm), <8 nm (>285 nm) |
| Dynamic range | 0 – 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength Correction | Yes |
| Monochromator Wavelength accuracy | ±2 nm |
| Wavelength repeatability | ±0.2 nm |
| Reading speed (kinetic) | 96 wells: 11 seconds 384 wells: 22 seconds |
| Lamp | Xenon Flask |
| Wavelength range | 250-700nm ex 250-700 nm em |
| Bandpass | ≤18 nm (Excitation and Emission) |
| Sensitivity | Sodium Fluorescein in phosphate-buffered saline (PBS) DL ≤20 pM, top or bottom read Excitation 485nm, Emission 528nm Methylumbelliferone (MUB) in carbonate-bicarbonate buffer (CBB) DL ≤0.16 ng/mL, top read Excitation 360 nm, Emission 460 nm Propidium Iodide (PI) in PBS DL ≤62.5 ng/mL, bottom read Excitation 485 nm, Emission 645 nm |
| Reading Speed | 96 wells: 11 seconds 384 wells: 22 seconds |
| Sensitivity | ≤ 75 amol/well flash ATP in a 96-well plate |
| Temperature control | Four Zone incubation to 45 °C; variation+0.2 °C at 37 °C with Condensation Control |
| Shaking | Linear, orbital, double orbital |
| Control | Gen5 Software (Windows 7 Notebook) |
| Dimensions (W x D x H) | 18x15x14″ |
| Power | 100 – 240V AC 50/60HZ |
| Weight | 41 lbs. |
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