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GE AKTA Pilot Automated Pilot-Scale FPLC System for Bioprocess Chromatography
Product Description
The GE AKTA Pilot FPLC System is a robust, automated preparative liquid chromatography platform designed for pilot-scale purification, process development, and small-scale cGMP manufacturing in biotechnology and biopharmaceutical research. Ideal for scaling laboratory methods to clinical production, it supports flow rates from 4 to 400 ml/min, extendable to 800 ml/min in dual-pump mode, with a maximum operating pressure of 2 MPa (20 bar, 290 psi), enabling compatibility with a variety of chromatography columns and media.
Key technical features include:
- Dual-module system pump (P-907) for precise binary gradient formation essential in complex separations and method optimization.
- Single-module sample pump (P-908) optimized for accurate sample loading.
- Integrated air trap and dual air sensors protect chromatography columns by removing bubbles, ensuring reproducibility during scale-up and minimizing column damage.
- In-line detectors with self-calibration and self-testing:
- UV absorbance detection covering a wavelength range of 190–700 nm at 1 nm resolution, critical for monitoring target biomolecules.
- Sanitary pH electrode (validated range pH 2–12) with temperature compensation for accurate pH monitoring in bioprocess environments.
- Conductivity detector spanning 1 µS/cm to 999.9 mS/cm to monitor buffer and elution conditions.
- Stepper-motor-actuated valves for precise fluid routing and fraction collection, supporting automated workflows and integration with external devices.
- Operated through UNICORN software, providing seamless method development, real-time process control, gradient formation, and advanced data analysis. Predefined method templates expedite media scouting and purification protocol optimization.
Designed to comply with GLP and cGMP standards, the system’s biocompatible and hygienic design meets regulatory demands for clinical trial material production and early phase biomanufacturing. This makes it essential equipment for biotechnology research areas such as bioprocessing, molecular diagnostics, and synthetic biology, and widely adopted in scaling up molecular cloning, protein purification, and biopharmaceutical production pipelines.
The GE AKTA Pilot is the standard solution for researchers and process engineers requiring reliable, scalable liquid chromatography solutions critical for biomedical engineering and advanced cell imaging purification workflows.
Item Stats
Product details
| Flow Rate Range | 4 to 400 ml/min |
| Dual-Pump Mode Maximum Flow Rate | Up to 800 ml/min |
| Pressure Range | 0 to 2 MPa (20 bar, 290 psi) |
| Wavelength Range | 190 to 700 nm in steps of 1 nm, 3 wavelengths simultaneously |
| Conductivity Range | 1 uS/cm to 999.9 mS/cm |
| pH Range | 0 to 14 (specifications valid between 2 and 12) |
| Maximum Viscosity | 5 cP |
| Dimensions (W x D x H) | 29.5 x 21.3 x 35 in. (75 x 54 x 90 cm) |
| Weight | 251 lb. (114 kg) |
| Electrical Requirements | 100-240V, 50/60Hz, 800VA |
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Northeastern UniversityFrom €42,940.22*ex. VAT
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