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GE AKTA FPLC Explorer 100 Air System with Multi-Module Purification Capabilities
Product Description
The GE AKTA FPLC Explorer 100 Air System is designed for high-performance protein purification with the following features:
- Wavelength Range: 190–700 nm, 1 nm steps
- Wavelength Accuracy: ± 2 nm
- Linearity: < 2% deviation up to 2 AU at 260 nm using Uracil at pH 2
Integrated Modules:
- P-900 and P-950 Pumps: Support isocratic, gradient, dual-mode operation; flow rates up to 200 ml/min
- UV-900 Detector: Multi-wavelength monitoring, low noise, low drift
- pH/C-900 Module: Inline pH (range 1–13) and conductivity monitoring
- 925 Mixer: Interchangeable static and dynamic chambers for optimized mixing efficiency
- Frac-950 Fraction Collector: Wide flow rate range; supports microplates, tubes, and bottles
Construction Materials: PTFE, PEEK, quartz, titanium for broad chemical compatibility.
System Validation:
- Fully tested for flow rate accuracy, pressure sensor operation, leak-tightness, UV detection performance, pH/conductivity measurement, motor valve function, and fraction collection performance via manual commands.
- Compatible with Unicorn 5.31 operating system for instrument control.
The system is modular, allowing tailored configurations for diverse purification protocols.
Item Stats
Product details
| Wavelength range | 190–700 nm in steps of 1 nm, 3 wavelengths simultaneously |
| Bandwidth | 4 nm |
| Wavelength accuracy | ±2 nm |
| Wavelength reproducibility | < 500 ms (one cycle from 214 nm to 254 nm and back to 214 nm) |
| Linearity | < 2% deviation up to 2 AU at 260 nm with Uracil at pH 2 |
| Static noise | short term: < 6x10-5 AU at 230 nm long term: < 6x10-5 AU at 230 nm |
| Drift | < 2x10-4 AU/h at 254 nm |
| Optical path length 2 mm cell | 2 mm, 2 µl cell volume |
| Optical path length 10 mm cell | 10 mm, 8 µl cell volume |
| Max. flow rate | 100 ml/min |
| Max. pressure | 2 MPa (290 psi) |
| Temperature range | +4 to +40 °C (liquid) |
| Wetted material | PTFE (polytetrafluoroethylene) PEEK (polyetheretherketone) titanium (palladium alloy), quartz (synthetic fused silica) and titanium |
| Flow Rate Range | Isocratic mode: 0.01 to 100 ml/min in steps of 10 ul/min Gradient mode: 0.01 to 100 ml/min in steps of 10 ul/min Double mode: 0.01 to 200 ml/min in steps of 10 ul/min |
| Pressure Range | 0 to 10 MPa (100 bar, 1450 psi) |
| pH Stability Range | 1 to 13 (1 to 14 with <1 day exposure) |
| Viscosity | Max 5 cP |
| Flow Rate Accuracy | ± 2% or 20 ul/min, whichever is greater |
| Flow Rate Reproducibility | Flow rate ≥ 0.5 ml/min: rsd < 0.5% |
| Gradient Composition | Accuracy: < ±1% at 0.05 to 10 ml/min Reproducibility: rsd < 0.25% at 0.5 to 100 ml/min |
| Pressure Sensor | Range: 0 to 27.5 MPa Offset error: < 0.05 MPa Scale error: < ±2% |
| Max Flow Rate | 0.001 to 100 ml/min |
| Max Pressure | 12ml chamber: 10 MPa at 0.1 to 100 ml/min (100 bar, 1450 psi) 0.6, 2, and 5ml chambers: 25 MPa at 0.001 to 100 ml/min (250 bar, 3625 psi) 90 ul and 0.2 ml chambers: 35 MPa at 0.001 to 5 ml/min (250 bar, 5075 psi) |
| pH Stability Range | 1 to 13 (1 to 14 with <1 day exposure) |
| Viscosity | Max 5 cP |
| Internal Volume | 6 interchangeable mixing chambers: 90 ul, 0.2, 0.6, 2, 5, and 12 ml |
| Mixing Principle | 1 static chamber and 1 dynamic chamber |
| Inlet and Outlet Tubing | UNF 10 to 32 thread profile |
| Max Flow Rate | 100 ml/min |
| pH Stability Range | 1 to 13 (1 to 14 with <1 day exposure) |
| Fraction Size | 0.1 to 99999.99 ml (volume mode) 0.1 to 99999.99 min (time mode) |
| Standard Mode | u-liter plates, DropSync: 0 to 1 ml/min 12 mm rack, DropSync: 0 to 1.5 ml/min 18 and 30 mm racks, DropSync: 0 to 2 ml/min 18 and 30 mm racks, Accumulator: 15 to 100ml/min |
| Prep Mode | 30 mm rack, Accumulator: 15 to 100 ml/min Funnel-to-flask, Accumulator: 15 to 100 ml/min250ml bottles, Accumulator: 15 to 100 ml/min |
| Environmental Conditions | +4° to +40°C 20 to 95% relative humidity84 to 106 kPa (840 to 1060 mbar) atmospheric pressure |
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