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Biotage Isolera Prime Flash Chromatography System with UV-Vis Detection and Fraction Collection
Product Description
The Biotage Isolera Prime Flash Chromatography System is a benchtop preparative chromatograph designed for efficient, high-throughput purification workflows. Featuring a single-channel, single-bed flash system equipped with a variable wavelength UV detector covering 200-400 nm or a fixed wavelength detector at 254 nm, it offers flexibility in chromatographic detection suitable for diverse molecular diagnostics and biotechnology applications.
Key technical features include:
- Flow rate range: 5–100 mL/min powered by a constant-volume electric high-performance flash chromatography (HPFC) pump.
- Pressure limit: 145 psi (10 bar) supporting robust separation tasks.
- UV detection: Variable or fixed wavelength detection modes enabling dual or single wavelength monitoring optimized for synthetic biology and molecular cloning separations.
- Fractionation modes: Multiple collection criteria such as volume, threshold, low/medium/custom slope, facilitating tailored isolation of target compounds.
- User interface: A solvent-resistant, 10.4” full-color touchscreen control panel with a window-based graphical interface allows real-time method adjustments and supports method transfer across systems, enhancing bioprocessing throughput.
- Compatibility: Supports a variety of sustainable cartridges including ZIP, SNAP, and high-performance options tailored to purification demands in biomedical engineering and molecular biology research.
- Dimensions and weight: Compact footprint (14x20x25 inches) and manageable weight (70 lbs) suited for laboratory bench space constraints.
Widely adopted in biotechnology workflows, the Isolera Prime system reduces method development time through its innovative TLC-to-Gradient feature, improving solvent usage efficiency for gene editing and biomanufacturing pipelines. It enables seamless integration into research environments requiring precision in fluorescence analysis and live-cell imaging preparative steps.
This flash chromatography system is essential for laboratories focusing on molecular diagnostics, synthetic biology, and biopharmaceutical production, offering reliable performance in purification of complex biological mixtures and small molecules critical for cutting-edge research.
Item Stats
Product details
| Wave length | 200-400 nm (PSV) or 254 nm fixed (PSF) |
| UV Detector | Variable(PSV) or Fixed(PSF) |
| Collector Bed | Single |
| Solvent Delivery | Constant volume electric HPFC pump |
| Flow Rate | 5–100 mL/min. |
| Pressure Limit | 145 psi (10 bar) |
| Flow Cell Path Length | 0.3 mm |
| UV Collection Modes | Single wavelength (Fixed) Dual wavelength (variable UV) |
| Fractionation Modes | Volume Threshold threshold with volume low slope medium slope custom slope |
| Collection Vessels | Test tubes (13, 16, 18 and 25 mm) Bottles (120, 240 and 480 mL) |
| Power | 100-240 VAC, 50/60 Hz, 4.0 A |
| System Control & Data Management | On-board computer with 10.4” diagonal touch screen interface |
| Dimensions | 14x20x25” |
| Weight | 70 lbs |
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