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Biotage Isolera Four Automated Flash Chromatography System with Variable/Fixed UV Detection
Product Description
The Biotage Isolera Four Flash Chromatography System is an automated purification platform designed for efficient compound isolation in synthetic chemistry and biotechnology research, including molecular diagnostics and bioprocessing workflows. This system features:
- UV Detection: Choice of a variable wavelength detector covering 200–400 nm or a fixed wavelength at 254 nm, enabling precise detection of UV-absorbing compounds critical in synthetic biology and molecular cloning workflows.
- Flow Rate & Pressure: Operates at flow rates from 1 to 200 mL/min with a pressure limit of 145 psi (10 bar), supporting a broad range of flash purification protocols, including gradient and stepwise elution methods.
- Sample Capacity: Capable of handling sample loads from milligrams up to 75 grams, suitable for both small-scale research and scale-up in biomanufacturing.
- System Configuration: Equipped with 4 solvent inlets and corresponding waste channels, allowing complex solvent gradients essential for separation of diverse biochemical compounds.
- Fraction Collection: Supports various fractionation modes (volume, threshold, slope-based) and multiple collection vessel sizes (test tubes and bottles up to 480 mL), facilitating downstream analysis and bioprocessing integration.
- Cartridge Compatibility: Optimized for Biotage cartridges such as SNAP, SNAP Ultra, ZIP, and ZIP Sphere ranging from 5g to 750g, enhancing reproducibility in chromatography-based purification critical for biotechnology applications.
- User Interface & Control: Features a built-in computer with a 10.4" touch screen, enabling intuitive protocol selection, including transfers between Isolera systems, which streamlines workflows in synthetic biology and molecular diagnostics laboratories.
- Technical Specifications: Utilizes dual piston, constant volume high-performance pumps ensuring consistent solvent delivery, supporting accurate fractionation and compound recovery essential to biopharmaceutical production pipelines.
- Dimensions & Power: Compact footprint (19x23x21 inches), weighing 105 lbs, powered by 100–240 VAC, 50/60 Hz, suitable for laboratory environments with limited space.
This flash chromatography system is widely adopted in synthetic biology research and critical in biopharmaceutical production pipelines, providing a reliable solution for automated, high-throughput purification in live-cell imaging and neurochemical analysis workflows. Its precise UV detection and modular cartridge compatibility ensure consistent purification results essential for advanced molecular biology and bioprocessing research.
Item Stats
Product details
| Wavelength | 200–400 nm -Variable Detector 4EV 254 nm Fixed Detector – 4EF |
| UV Detector | Variable – 4EV or Fixed – 4EF |
| Collector Bed | Extended |
| Solvent inlets | 4 |
| Cartridge Positions | 4 |
| Waste Channels | 4 |
| Solvent Delivery | Dual Piston, constant volume (3 mL) electric HPFC pumps |
| Flow Rate | 1–200 mL/min |
| Pressure Limit | 145 psi (10 bar) |
| Sample Interval | Milligrams–75 grams |
| UV Detection | Choice of variable wavelength (200–400 nm) |
| Flow Cell Path Length | 0.3 mm |
| Fractionation Modes | Volume, threshold, threshold with volume, low slope, medium slope, custom slope or via external detection |
| Compatible Collection Vessels | Test tubes (13 mm, 16 mm, 18 mm, and 25 mm) and bottles (120 mL, 240 mL, and 480 mL) |
| Power | 100–240 VAC, 50/60 Hz, 200VA |
| System Control & Data Management | On-board computer with 10.4” touch screen interface |
| Dimensions | 19x23x21” |
| Weight | 105lbs |
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