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Amersham Biosciences AKTA Purifier 10 Fast Protein Liquid Chromatography (FPLC) System with UPC-900 Monitor
Product Description
The Amersham Biosciences AKTA Purifier 10 FPLC System is a modular and versatile liquid chromatography platform designed for the purification of proteins, peptides, and nucleic acids, supporting microgram to milligram scale bioprocessing workflows. Operating within a flow rate range of 0.001 to 10 ml/min and pressure range of 0 to 25 MPa, it ensures precise and reproducible separations essential for molecular diagnostics and biotechnology research.
Key technical features include:
- P-900 high-precision pump with validated flow rate accuracy critical for consistent chromatographic performance
- UPC-900 Monitor for real-time UV absorbance detection across a 0.01 to 5 AU full-scale range, facilitating sensitive protein and peptide quantification
- Seamless integration with Unicorn 5.31 operating system for comprehensive control and data collection, supporting advanced chromatography protocols
- Functional communication with pH probe and Conductivity Cell (C-900) for buffer monitoring, enhancing process control in biomanufacturing and synthetic biology applications
- Tested motor valves, mixers, and Frac-920 fraction collector support high-throughput separations and downstream analysis
- Suitable for purification workflows in protein engineering, gene editing, and biopharmaceutical production pipelines
This system is widely adopted among molecular biology and biotechnology researchers for automated fast protein liquid chromatography, offering reproducible separations critical in bioprocess development and synthetic biology. The AKTA Purifier 10 supports applications including live-cell protein isolation, peptide fractionation, and nucleic acid purification, making it an essential component in molecular cloning and molecular diagnostics laboratories.
Note: pH probe and holder are available optionally at additional cost.
Item Stats
Product details
| Flow Rate Range | Isocratic mode: 0.001 to 10 ml/min in steps of 1 ul/min Gradient mode: 0.001 to 10 ml/min in steps of 1 ul/min Double mode: 0.001 to 20 ml/min in steps of 1 ul/min |
| Pressure Range | 0 to 25 MPa (100 bar, 1450 psi) |
| pH Stability Range | 1 to 13 (1 to 14 with <1 day exposure) |
| Viscosity | Max 5 cP |
| Flow Rate Accuracy | ± 2% or 20 ul/min, whichever is greater |
| Flow Rate Reproducibility | Flow rate ≥ 0.5 ml/min: rsd < 0.5% |
| Gradient Composition | Accuracy: < ±1% at 0.05 to 10 ml/min Reproducibility: rsd < 0.25% at 0.5 to 100 ml/min |
| Pressure Sensor | Range: 0 to 27.5 MPa Offset error: < 0.05 MPa Scale error: < ±2% |
| Total Volume Between Inlet and Outlet | < 800 ul/module |
| Absorbance range | 0.01 - 5 AU (full scale) |
| Autozero range | -0.2 - 2.0 AU |
| Baseline adjust Adjustable | 0-100% of full scale |
| Wavelengths | Hg lamp, fixed: 254 and 280 by changing filter:313, 365, 405, 436 and 546 nm Zn lamp: 214 nm |
| Linearity, 5 mm cell | <3% up to 2 AU at 254 nm <5% up to 1 AU at 280 nm |
| Static noise | short term: 40x10-6 AU at 254 nm (typically 6x10-6 AU at 254 nm) long term: 40x10-6 AU at 254 nm |
| Static Drift | ±100x10-6 AU/h at 254 nm |
| Flow Sensitivity | 2x10-4 AU min/ml |
| Max Flow Rate | 0.001 to 10 ml/min |
| Max Pressure | 12ml chamber: 10 MPa at 0.1 to 100 ml/min (100 bar, 1450 psi) 0.6, 2, and 5ml chambers: 25 MPa at 0.001 to 100 ml/min (250 bar, 3625 psi) 90 ul and 0.2 ml chambers: 35 MPa at 0.001 to 5 ml/min (250 bar, 5075 psi) |
| pH Stability Range | 1 to 13 (1 to 14 with <1 day exposure) |
| Viscosity | Max 5 cP |
| Internal Volume | 6 interchangeable mixing chambers: 90 ul, 0.2, 0.6, 2, 5, and 12 ml |
| Mixing Principle | 1 static chamber and 1 dynamic chamber |
| Inlet and Outlet Tubing | UNF 10 to 32 thread profile |
| Capacity | 95 fractions in 10-18 mm tubes |
| Depth | 400 mm |
| Flow Rate | < 100 ml/min |
| Height | 250 mm |
| Operating Temperature | 4° to 40°C |
| pH Stability Long Term | 1 – 13 |
| pH Stability Short Term | 1 – 14 |
| Program Memory | 99 programs |
| Rack Designation | 95 x 10-18 mm (Standard) 175 x 12 mm and 40 x 30 mm (Optional) |
| Relative Humidity | 20 to 95% (non condensing) |
| Time Mode | 0.01 to 9.999 min |
| Volume Mode | 0.1 to 999 ml |
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