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ÄKTA oligopilot plus 100 Automated Oligonucleotide Synthesizer for High-Scale DNA/RNA Synthesis
Product Description
The ÄKTA oligopilot plus 100 is a fully automated oligonucleotide synthesizer engineered for small to medium-scale DNA and RNA synthesis, employing advanced flow-through reactor technology. Designed primarily for research, process development, and biopharmaceutical applications, it supports flexible synthesis scales ranging from 50 μmol to 9 mmol, enabling cost-efficient synthesis of high-purity oligonucleotides essential in molecular diagnostics, gene editing, and synthetic biology.
Key technical features include:
- Flow-through synthesis technology, providing exact control of reaction rates and contact times, facilitating easy scale-up while significantly reducing reagent consumption.
- Seven column positions and up to twelve monomer reagent positions allow adaptable synthesis protocols compatible with diverse oligonucleotide chemistries including RNAi, siRNA, aptamers, antisense oligonucleotides, immunostimulatory CpG motifs, and exon skipping therapeutics.
- Column re-circulation capability, crucial for extended coupling reactions typical in RNA or modified amidite synthesis, ensuring optimal reaction yields and product quality.
- Pressure-controlled flow and UV-based conditional programming, enabling rapid column washing and minimizing cycle times to enhance throughput and reproducibility.
- Compatibility with a wide range of column reactors from fixed volumes (1.2 mL to 48 mL) to larger adjustable FineLINE 35 oligo columns (250 μmol to 4 mmol) and adjustable columns (<9 mmol), offering flexibility for various bioprocess development needs.
- Integration with UNICORN 21 CFR Part 11 compatible control software, featuring a sequence editor supporting up to 200 bases, multi-level password protection, lot number registration, full synthesis documentation, and real-time process monitoring for PAT (Process Analytical Technology) compliance. Online trityl and coupling efficiency monitors enhance synthesis security by halting operations if quality thresholds are breached.
- Supports cGMP and GLP compliant batch operations, facilitating its use in regulated environments pertinent to biomanufacturing and molecular biology research workflows.
This synthesizer is widely adopted in biotechnology laboratories focused on synthetic biology, molecular cloning, and bioprocessing, where precise, scalable, and reproducible oligonucleotide production is critical. Its automated workflow optimizes laboratory efficiency in clinical diagnostics, therapeutic oligonucleotide development, and advanced research in nucleic acid chemistry.
Item Stats
Product details
| Manufactured | Please ask. |
| Location | Gräfelfing, Bavaria, Germany |
| Specification | refurbished |
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