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Bruker LC-NMR-MS Interface
The LC-NMR-MS Interface is designed to sit between an HPLC system, an NMR spectroscopy probehead and an MS unit. It would normally be mounted on top of the MS table immediately in front of the MS input connector.
The BNMI-HP consists of 3 medium pressure (> 60Bar) syringe pumps, a 1:20 splitter, an 8-Port valve, 3 mixing chambers (T-Pieces) and associated control and interface electronics. The flow of the HPLC separations to the MS unit can be modified by pumping additional solvent from the syringe pumps. This improves the MS peak detection and shortens the time needed for the split separation to reach the MS. Separations can also be stored as a result of an MS peak detection as well as through UV absorption peak detection.
The splitter splits the fluid flow from the HPLC in a 1:20 ratio. i.e. ca. 5% of the fluid is directed to the MS and the remainder to either the NMR probehead for immediate analysis or to the BPSU36 storage cassette for storage and later analysis.
The fluid directed to the MS is mixed in the mixing chamber (T-Piece) with the solvent pumped from the syringe pumps. The two left hand syringe pumps pump alternatively to guarantee a constant ’makeup’ flow. The 3rd pump at the right hand side is used to pump a calibration fluid into the flow path to allow the MS to be calibrated (or simply synchronized) at the start and end of each measurement cycle.
The 8-Port valve is used to direct the fluid flow. Control of the BNMI Unit is performed using the HyStar program via an ethernet interface. Commands received from HyStar are interpreted in the BNMI Control Board. The control board then sets up the BNMI unit accordingly e.g.: Starts the pumps, changes the flow rate, turns the 8-Port valve. The splitter has a fixed mechanical function and cannot be controlled or adjusted. It simply accepts a fluid flow and splits it into 2 paths in a 1:20 ratio. However, other split ratios are available (for example 1:100 may be preferred if you have very high sample concentrations).
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