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Agilent 5200 Fragment Analyzer Unit 2 - Capillary Electrophoresis DNA Analysis System
Product Description
The Agilent 5200 Fragment Analyzer Unit 2 is an advanced fluorescence-based capillary electrophoresis instrument designed for high-precision nucleic acid fragment analysis essential in genomics, molecular diagnostics, and biotechnology research. This system enables low- to medium-throughput processing, analyzing up to 288 samples across three 96-well microplates with a rapid cycle time of approximately 15 minutes per 12 samples in parallel.
Key Technical Features:
- Analysis Method: Fluorescence-based capillary electrophoresis using intercalating dyes for sensitive detection of DNA and RNA fragments.
- Capillary Array Compatibility: Supports 12-capillary arrays, facilitating simultaneous fragment separation.
- Sample Requirements: Minimum sample volume of 20 µL for liquid injection, requiring as little as 1 µL of actual nucleic acid sample.
- Sample Capacity: Accommodates three sample trays, each with 96-well PCR microplate capacity (semi or non-skirted), suitable for marker and sample handling, plus 96 deep-well plates for buffers and waste.
- Sensitivity and Accuracy: Detects fragment concentrations as low as 5 pg/µL, with sizing resolution down to 3 base pairs for fragments under 300 bp, critical for mutation detection and microsatellite analysis.
- Applications: Widely used in next-generation sequencing (NGS) fragment analysis, plasmid and large genomic DNA characterization, microsatellite/SSR genotyping, reverse genetics, and mutation screening via TILLiNG approaches.
- Throughput: Variable throughput system ideal for medium-scale molecular biology workflows, including gene editing and synthetic biology projects requiring reliable fragment sizing and quantification.
- Software and Control: Operated via dedicated computer running Fragment Analyzer controller and ProSize data analysis software for comprehensive data acquisition and interpretation.
Instrument Reliability and Maintenance:
- Automated wash system and long-life capillaries minimize user intervention and downtime.
- Validated for stable power supply, precise fluidic pump operation, smooth plate transport, and proper optical alignment, ensuring consistent data quality and reliability.
- Includes remote instrument setup and training, plus a 120-day continental US warranty and 60-day international warranty.
Biotechnology Relevance:
This instrument is critical in biopharmaceutical production pipelines, molecular cloning workflows, and synthetic biology research, providing essential fragment sizing data for nucleic acid quality control, helping to streamline bioprocessing and molecular diagnostics tasks. Its sensitivity and high resolution make it popular among molecular biology researchers requiring precise detection of mutations and fragment variations, supporting advanced applications in biomedical engineering and gene editing.
Includes:
- Agilent 5200 Fragment Analyzer hardware
- Marker/sample PCR microplate holders (4 semi/non-skirted)
- Deep-well microplate holders (2)
- Sample and waste bottles (3x 50 mL, 1x 500 mL)
- Communication and power cables
- Computer with pre-installed control and analysis software
Note: Capillary arrays and other consumables are sold separately.
The Agilent 5200 Fragment Analyzer Unit 2 is designed for researchers and laboratory professionals focused on nucleic acid analysis, fragment sizing, and quality control in genomics and biotechnology fields, offering robust performance and dependable results for fragment analysis workflows.
Item Stats
Product details
| Stock | In Stock |
| Brand | Agilent |
| Product Code | SM101493-5200-U2 |
| Condition | Pre-Owned |
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