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The Thermo ImmunoCAP Phadia 1000 Immunoassay Analyzer is an advanced in vitro testing system designed for the precise quantification of allergen-specific IgE in human serum or plasma. It serves as an essential tool for in vitro diagnostic applications, aiding in the clinical assessment of IgE-mediated allergic conditions when used alongside other clinical evaluations, specifically within clinical laboratory settings.
The analytical process begins with the allergen of interest, which is covalently attached to the ImmunoCAP, initiating a reaction with specific IgE present in the patient sample. Subsequent to the removal of non-specific IgE through washing, enzyme-labeled anti-IgE antibodies are introduced, forming a detectable complex. The incubation period allows for the binding process to occur, after which any unbound enzyme-anti-IgE is washed away. The bound complex is then treated with a developing agent. Upon halting the reaction, the resulting fluorescence of the eluate is measured. The intensity of the response value correlates with the concentration of specific IgE present in the sample. For accurate interpretation, the patient sample responses are converted into precise concentrations utilizing a calibration curve.
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