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Marketplace
Analytics
Brand
Amersham
Supplier
LabMakelaar Benelux B.V.
Location
Zevenhuizen - Netherlands
This item is
Not available
State of item
used
Categories

Amersham Biosciences ÄKTAxpress FPLC System

LabMakelaar Benelux B.V.
item image

Description by the supplier

ÄKTAxpress is a liquid chromatography system intended for automated, multi steppurification processes. The system has been developed and optimized for purificationof Histidine-tagged and Glutathione S-Transferase (GST)-tagged recombinant proteins from clarified or crude cell lysates. ÄKTAxpress supports a number of purification protocols containing up to four chromatography steps, for example, affinity chromatography followed by desalting, ion exchange, and gel filtration. Protocols for purification of both low expressed proteins and well expressed proteins are included. Also protocols supporting purification of double tagged proteins and protocols that can be combined with on-column and off-line (batch) tag cleavage are provided.


ÄKTAxpress provides the following features:


•Automatic purification of up to four samples in a single run.


•Purification protocols containing up to four chromatography steps.


•Automated on-column affinity tag removal in the first step of purification protocols starting with an affinity/ion exchange chromatography step.


•Purification protocols supporting purification of both well expressed and low expressed proteins, double tagged proteins and proteins that have been cleaved off-line.


•Up to 50 mg of protein can be purified per sample.


•Up to twelve ÄKTAxpress systems can be run in parallel, controlled from one computer (not included). Different purification protocols can be run simultaneously.


•The systems can be placed next to each other or they can stand separately if preferred.


Flow rate: 0.1 – 65 mL/min


Flow rate accuracy: ±2% or ±0.02 ml/min whichever is greater


Flow rate reprocibility: RSD < 0.5%


Pressure range: 0 – 30 bar


Step gradients (quasi-isocratic mixing) formation conditions: Water based buffers with different concentrations of salts


Gradient mmix proportions: 5 – 95%


Concentration accuracy: ±2%B


Linear gradients conditions: Water based buffers with different concentrations of salts


Linearity: ±2%B within 20 – 80%B


UV monitor wavelengths: 280 and 254 nm set by lamp position and filter


Optical path length: 2 nm


Opitcal cell volume: 2 µL


Detector cell volume: 30 µL


Absorbance range: 0.01 – 5 AU


Auto zero range: -0.2 – 2.0 AU


Linearity, deviation: < 3% up to 2 AU


Static noise, short and long term: < 40 μAU


Static drift: ±0.1 mAU/h


Conductivity range: 0.01 – 300.00 mS/cm


Accuracy: Max. ±2% of full scale calibrated range or ±0.1 mS/cm whichever is greater


Reproducibility, short and long term: Max. ±1% or ±5 μS/cm whichever is greater


Noise: Max. ±0.5% of full scale calibrated range, typically ±0.1%


Response time: Max. 4 s (0–95% of step)


Internal volume: 24 μl


Dimensions (WxdxH): 250 x 490 x 660 mm


Weight: 30 kg

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