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Amersham Biosciences ÄKTAxpress FPLC System
The Amersham Biosciences ÄKTAxpress FPLC System is a pre-owned liquid chromatography platform specifically designed for automated, multi-step purification processes. This system is optimized for the purification of Histidine-tagged and Glutathione S-Transferase (GST)-tagged recombinant proteins, accommodating clarified or crude cell lysates seamlessly. It supports a variety of purification protocols, incorporating up to four chromatographic steps such as affinity chromatography, desalting, ion exchange, and gel filtration.
Key features of the ÄKTAxpress include:
- Automated purification of up to four samples in a single run.
- Versatile purification protocols with up to four chromatography steps.
- Integrated on-column affinity tag removal during initial affinity/ion exchange chromatography steps.
- Comprehensive protocols catering to both well-expressed and low-expressed proteins, including double-tagged proteins and those cleaved off-line.
- Capacity to purify up to 50 mg of protein per sample.
- Capability to operate up to twelve systems in parallel, governed by a single computer (not included), supporting concurrent execution of different purification protocols.
- Flexible system placement, either adjacent or separated depending on laboratory requirements.
Technical Specifications:
- Flow rate range: 0.1 – 65 mL/min
- Flow rate accuracy: ±2% or ±0.02 ml/min, whichever is greater
- Flow rate reproducibility: RSD < 0.5%
- Pressure range: 0 – 30 bar
- Gradient mixing conditions: Water-based buffers with varying salt concentrations, proportions of 5 – 95%
- Concentration accuracy: ±2%B
- UV monitoring wavelengths: 280 and 254 nm, adjustable via lamp positioning and filter
- Optical path length: 2 nm, with a cell volume of 2 µL and a detector cell volume of 30 µL
- Absorbance range: 0.01 – 5 AU, with an auto zero range of -0.2 – 2.0 AU
- Linearity and deviation constraints of < 3% up to 2 AU
- Static noise and drift factors: < 40 μAU and ±0.1 mAU/h, respectively
- Conductivity range and accuracy: 0.01 – 300.00 mS/cm, with a maximum ±2% of full-scale calibrated range accuracy or ±0.1 mS/cm
- Noise parameter: Maximum ±0.5% of full scale calibrated range, typically ±0.1%
- Response time: Maximum 4 seconds (0–95% of step)
- Internal volume: 24 µl
- Dimensions (W x D x H): 250 x 490 x 660 mm
- Weight: 30 kg
The ÄKTAxpress FPLC System is a robust choice for laboratories requiring reliable and precise protein purification capabilities.
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