Amersham Biosciences ÄKTAxpress FPLC System

The Amersham Biosciences ÄKTAxpress is an advanced liquid chromatography system designed for automated, multi-step purification processes. This system is optimized for the purification of Histidine-tagged and Glutathione S-Transferase (GST)-tagged recombinant proteins from either clarified or crude cell lysates. With protocols supporting affinity chromatography, desalting, ion exchange, and gel filtration, the ÄKTAxpress caters to both low and well-expressed proteins. Additionally, it accommodates double-tagged proteins and can integrate on-column and offline tag cleavage procedures.

Key features of the ÄKTAxpress include:

• Automatic purification of up to four samples per run
• Protocols with up to four chromatography steps
• On-column affinity tag removal in the initial step
• Purification capacities of up to 50 mg of protein per sample
• Simultaneous operation of up to twelve systems controlled from a single computer, with diverse purification protocols
• Flexible system placement

Technical specifications include:

• Flow rate: 0.1 – 65 mL/min (accuracy: ±2% or ±0.02 mL/min)
• Pressure range: 0 – 30 bar
• Step gradient conditions: Water-based buffers with varying salt concentrations
• Gradient mix proportions: 5 – 95%
• UV monitor wavelengths: 280 and 254 nm
• Optical path length: 2 nm, cell volume: 2 µL
• Detector cell volume: 30 µL, absorbance range: 0.01 – 5 AU
• Conductivity range: 0.01 – 300.00 mS/cm (accuracy: ±2% or ±0.1 mS/cm)
• Dimensions: 250 x 490 x 660 mm, weight: 30 kg

The ÄKTAxpress is an ideal solution for dynamic and efficient protein purification workflows, enhancing both capability and convenience in the laboratory.