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Introducing the Amersham Biosciences ÄKTAxpress FPLC System, a pre-owned, advanced liquid chromatography platform designed for the automated, multi-step purification of proteins. This system is specifically tailored for the efficient isolation of Histidine-tagged and Glutathione S-Transferase (GST)-tagged recombinant proteins from both clarified and crude cell lysates. The ÄKTAxpress system is capable of executing up to four sequential purification steps, which may include affinity chromatography, ion exchange, desalting, and gel filtration, thereby accommodating a broad range of purification protocols.
Key Features:
- **Automated Purification:** Capable of processing up to four samples in a single, unattended run.
- **Versatile Protocols:** Supports purification protocols encompassing up to four chromatographic steps, suitable for proteins expressed at varying levels, including double-tagged proteins and those undergoing on-column or batch tag cleavage.
- **Sample Capacity:** Purifies up to 50 mg of protein per sample efficiently.
- **Scalability:** Operate up to twelve ÄKTAxpress systems in parallel, managed by one computer (computer not included), with the flexibility to use distinct purification protocols concurrently.
- **Flexible Configuration:** Systems can be configured adjacently or separately based on lab requirements.
Technical Specifications:
- **Flow Rate:** 0.1 – 65 mL/min with an accuracy of ±2% or ±0.02 mL/min, whichever is greater. Flow reproducibility is marked by RSD < 0.5%.
- **Pressure Range:** Operates effectively within a pressure range of 0 – 30 bar.
- **Gradient Formation:** Capable of quasi-isocratic mixing with water-based buffers and salt concentrations ranging from 5 – 95%, ensuring a concentration accuracy of ±2%B.
- **UV Monitoring:** Equipped with dual-wavelength monitoring at 280 and 254 nm facilitated by adjustive lamp positioning and filtering.
- **Optical Specifications:** Includes an optical path length of 2 nm, optical cell volume of 2 µL, and a detector cell volume of 30 µL; absorbance range is from 0.01 – 5 AU.
- **Conductivity Range:** 0.01 – 300.00 mS/cm with maximum accuracy of either ±2% of the full scale or ±0.1 mS/cm.
- **Compact Design:** The system's dimensions are 250 x 490 x 660 mm with a manageable weight of 30 kg.
Embrace automation and precision in your protein purification processes with the ÄKTAxpress, designed to meet the rigorous demands of modern biochemistry and molecular biology laboratories.
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