Roche Light Cycler 96 Real-Time PCR System with Integrated PC

The Roche Light Cycler 96 is a high-performance real-time PCR system designed for rapid and sensitive amplification and quantitative analysis of nucleic acids. It features a 96-well block capable of running standard 0.1 mL tubes, 8-strip tubes, or 96-well plates, supporting reaction volumes from 10 to 50 µL. The instrument offers fast thermal cycling with a maximum heating rate of 4.4 °C/sec and cooling rate of 2.2 °C/sec, controlled precisely within ±0.2 °C accuracy and ±0.3 °C uniformity, suitable for robust amplification protocols.

Key fluorescence detection relies on high-power broad spectrum LED excitation at four distinct wavelengths (470, 533, 577, 645 nm) paired with fixed detection wavelengths, facilitating multiplex capabilities for dyes such as SYBR Green I, FAM, VIC, HEX, Texas Red, and Cy5 without the need for ROX correction due to the innovative glass fiber-based detection system. The system includes a 96 x 2 CCD camera detector array with individual well glass fiber optics for superior sensitivity and reproducibility, essential for quantitative PCR, high-resolution melt (HRM) analysis, and genotyping.

The Light Cycler 96 supports LAN connectivity and USB data transfer, eliminating the need for dedicated PCs during operation via a user-friendly touch panel interface, enabling streamlined workflows in molecular diagnostics, biotechnology research, and clinical applications. Compatible with Windows operating systems from XP through 11, it offers comprehensive data analysis including absolute and relative quantification, Tm calling, endpoint genotyping, and qualitative detection methods.

Physically, the unit measures 40 cm width, 40 cm depth, and 53 cm height, weighing approximately 27 kg, powered by an AC 100–125 V, 600 W supply, making it suitable for benchtop use in research and diagnostic laboratories focused on molecular diagnostics, bioprocessing, and synthetic biology applications. This unit is offered in used condition and includes the operation manual, with warranty coverage for repairs or replacement within a specified period.