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Amersham Biosciences AKTA Purifier 100 Fast Protein Liquid Chromatography System with UV-900 Detector
Product Description
The Amersham Biosciences AKTA Purifier 100 FPLC System equipped with the UV-900 Detector is a modular and versatile fast protein liquid chromatography platform designed for precise purification of proteins, peptides, and nucleic acids. Engineered for microgram to milligram scale separations, it operates at flow rates from 0.01 to 100 ml/min and pressures up to 10 MPa, ensuring optimized performance for biotechnology workflows including molecular diagnostics, synthetic biology, and bioprocessing applications.
Key technical features include:
- P-900 Pump delivering accurate flow rates critical for reproducible chromatography and biomanufacturing processes
- PHC-900 Monitor compatible with pH and conductivity probes, enabling inline buffer condition monitoring essential for biochemical separations
- Support for Frac-920 Fraction Collector, facilitating high-throughput sample collection in protein purification and molecular cloning workflows
- Seamless integration with the Unicorn 5.31 operating system for automated gradient control, method programming, and data acquisition
This system undergoes rigorous functional testing including pump flow accuracy, pH and conductivity sensor communication, motor valve operation, and fraction collector responsiveness, ensuring reliable operation in research and industrial biotechnology environments. The optional pH probe and holder are available separately to enhance functionality in applications requiring precise buffer condition monitoring.
Widely adopted in biotechnology research and biopharmaceutical production pipelines, the AKTA Purifier 100 supports advanced purification protocols crucial for structural biology, live-cell imaging sample preparation, and protein characterization. Its adaptability and robust performance make it an essential instrument for laboratories specializing in molecular biology, cell imaging, and synthetic biology research.
Item Stats
Product details
| Flow Rate Range | Isocratic mode: 0.01 to 100 ml/min in steps of 10 ul/min Gradient mode: 0.01 to 100 ml/min in steps of 10 ul/min Double mode: 0.01 to 200 ml/min in steps of 10 ul/min |
| Pressure Range | 0 to 10 MPa (100 bar, 1450 psi) |
| pH Stability Range | 1 to 13 (1 to 14 with <1 day exposure) |
| Viscosity | Max 5 cP |
| Flow Rate Accuracy | ± 2% or 20 ul/min, whichever is greater |
| Flow Rate Reproducibility | Flow rate ≥ 0.5 ml/min: rsd < 0.5% |
| Gradient Composition | Accuracy: < ±1% at 0.05 to 10 ml/min Reproducibility: rsd < 0.25% at 0.5 to 100 ml/min |
| Pressure Sensor | Range: 0 to 27.5 MPa Offset error: < 0.05 MPa Scale error: < ±2% |
| Total Volume Between Inlet and Outlet | < 800 ul/module |
| Wavelength range | 190–700 nm in steps of 1 nm, 3 wavelengths simultaneously |
| Bandwidth | 4 nm |
| Wavelength accuracy | ±2 nm |
| Wavelength reproducibility | < 500 ms (one cycle from 214 nm to 254 nm and back to 214 nm) |
| Linearity | < 2% deviation up to 2 AU at 260 nm with Uracil at pH 2 |
| Static noise | short term: < 6x10-5 AU at 230 nm long term: < 6x10-5 AU at 230 nm |
| Drift | < 2x10-4 AU/h at 254 nm |
| Max Flow Rate | 0.001 to 10 ml/min |
| Max Pressure | 12ml chamber: 10 MPa at 0.1 to 100 ml/min (100 bar, 1450 psi) 0.6, 2, and 5ml chambers: 25 MPa at 0.001 to 100 ml/min (250 bar, 3625 psi) 90 ul and 0.2 ml chambers: 35 MPa at 0.001 to 5 ml/min (250 bar, 5075 psi) |
| pH Stability Range | 1 to 13 (1 to 14 with <1 day exposure) |
| Viscosity | Max 5 cP |
| Internal Volume | 6 interchangeable mixing chambers: 90 ul, 0.2, 0.6, 2, 5, and 12 ml |
| Mixing Principle | 1 static chamber and 1 dynamic chamber |
| Inlet and Outlet Tubing | UNF 10 to 32 thread profile |
| Capacity | 95 fractions in 10-18 mm tubes |
| Depth | 400 mm |
| Flow Rate | < 100 ml/min |
| Height | 250 mm |
| Operating Temperature | 4° to 40°C |
| pH Stability Long Term | 1 – 13 |
| pH Stability Short Term | 1 – 14 |
| Program Memory | 99 programs |
| Rack Designation | 95 x 10-18 mm (Standard) 175 x 12 mm and 40 x 30 mm (Optional) |
| Relative Humidity | 20 to 95% (non condensing) |
| Time Mode | 0.01 to 9.999 min |
| Volume Mode | 0.1 to 999 ml |
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