Agilent Series 1100 HPLC System

System contains:

Solvent tray

Vacuum degasser: G1322A

Vacuum degasser:

The Agilent 1100 HPLC Degasser features a 4-channel vacuum container, including four tubular plastic membranes, and a vacuum pump. This degasser has a maximum flow rate of 0-10ml/min flow rate per channel and 5-10ml/min at reduced degassing performance.

Quaternary pump:

Hydraulic system: Dual plunger in series pump with proprietary servo-controlled variable stroke drive, floating plungers and active inlet valve

Setable flow range: 0.001 – 10 ml/min, in 0.001 ml/min increments

Flow range: 0.2 – 10.0 ml/min

Flow precision: < 0.07% RSD, or < 0.02 min SD whatever is greater, based on retention time at constant room temperature

Flow accuracy: ± 1% or 10 µl/min whatever is greater

Pressure: Operating range 0 – 400 bar up to 5 ml/min, 0 – 200 bar up to 10 ml/min

Pressure pulsation: < 2 % amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 10 bar

Compressibility compensation: User-selectable, based on mobile phase compressibility

Recommended pH range: 1.0 – 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel

Gradient formation: Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve Delay volume 800 – 1100 µl, dependent on back pressure

Composition Range: 0 – 95 % or 5 – 100 %, user selectable

Composition Precision: < 0.2 % RSD, at 0.2 and 1 ml/min

Autosampler thermostat:

Temperature range: setable from 4°C to 40°C in 1°C increments

Temperature accuracy (at ambient temperatures < 25 °C and humidity < 50%): – 1°C to + 4°C at a setpoint of 4°C

Temperature accuracy (at ambient temperatures > 25 °C and/or humidity > 50%): – 1°C to + 5°C at a setpoint of 4°C

Standard autosampler:

Pressure: Operating range 0 – 400 bar

GLP features: Early maintenance feedback (EMF), electronic records of maintenance and errors

Safety features: Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display

Injection range: 0.1 – 100 µl in 0.1 µl increments

Replicate injections: 1 – 99 from one vial

Precision: <0.25% RSD from 5 – 100 µl, <1% RSD 1 – 5 µl

Minimum sample volume: 1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial

Carryover: Typically <0.1%

Sample viscosity range: 0.2 – 50 cp

Replicate injections per vial: 1 – 99

Sample capacity 100 x 2 ml vials in 1 tray

Injection cycle time: Typically 50 s depending on draw speed and injection volume

Thermostatted colomn compartment:

Temperature range: 10 degrees below ambient to 80 °C, up to 80 °C: flow rates up to 5 ml/min

Temperature stability: ± 0.15 °C

Temperature accuracy: ± 0.8 °C (± 0.5 °C With calibration)

Column capacity: Three 30 cm

Warm-up/cool-down time: 5 minutes from ambient to 40 °C, 10 minutes from 40 – 20 °C

Dead volume: 3 µl

Communications: Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN via other series module

Safety and maintenance: Extensive diagnostics, error detection and display (through control module and Agilent ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.

Diode array detector:

Detection type: 1024-element photodiode array

Light source: Deuterium and tungsten lamps

Wavelength range: 190 – 950 nm

Short term noise (ASTM) Single and Multi-Wavelengt: ± 1 × 10-5 AU at 254 and 750 nm

Drift: 2 × 10-3 AU/hr at 254 nm

Linear absorbance range: > 2 AU (upper limit)

Wavelength accuracy: ± 1 nm Self-calibration with deuterium lines, verification with holmium oxide filter

Wavelength bunching: 1 – 400 nm Programmable in steps of 1 nm

Slit width: 1, 2, 4 , 8, 16 nm Programmable slit

Diode width: < 1 nm

Flow cells: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum

Control and data evaluation: Agilent ChemStation for LC

Analog outputs: Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, two outputs

Communications: Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional

Safety and maintenance: Extensive diagnostics, error detection and display through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.

GLP features: Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter.

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