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Agilent 6545A Q-TOF Mass Spectrometer with 1200 LC System
Product Description
The Agilent 6545A Q-TOF Mass Spectrometer combined with the 1200 LC System delivers advanced mid-range quadrupole time-of-flight mass spectrometry optimized for small molecule analysis.
Key technical specifications:
- Mass range: m/z 100 to 10,000
- Mass accuracy: <0.8 ppm RMS
- Spectral acquisition rate: 50 spectra/second (MS mode), 30 MS/MS spectra/second (MS/MS mode)
- Dynamic range: Up to five decades
- Ion mode switching: Positive/negative ion modes with 1.5-second cycle time
Instrument features:
- Up to 5x sensitivity improvement over prior models
- Fast autotune functionality for optimized performance and enhanced small molecule detection
- Robust ion optics and beam shaping technology improve maintenance intervals and analytical consistency
- New high-voltage pulsers and power supplies boost mass resolution and accuracy
- New-generation detector with improved longevity and wide dynamic range
- Advanced MassHunter software with powerful data-mining tools
- Seamless positive/negative mode switching to improve workflow efficiency
1200 LC System diagnostics include:
- Variable Wavelength Detector tests (filter, dark current, holmium oxide spectrum, lamp intensity, wavelength accuracy, baseline noise)
- Binary Pump tests (pressure to 400 bar, leak-tightness)
- Column Compartment thermostat performance test
- Autosampler injector step functional test
- System-wide 4 Peak Test to verify overall LC performance
The system is factory recertified and calibrated, ready for immediate shipment, with all installation components included. This platform suits pharmaceutical, environmental, food safety, metabolomics, and lipidomics research demanding precise, high-throughput small molecule analysis.
Item Stats
Product details
| Sensitivity, MS Mode, Electrospray On-Column, 400 uL/min Flow Rate | 500:1 RMS (1 pg LC/MS injection of reserpine. Signal-to-noise for the reserpine (M+H)+ at m/z 609.2807 while maintaining a resolution of 40,000 at m/z 2,722 in 4 GHZ mode) |
| Sensitivity, MS/MS Mode, Electrospray On-Column, 400 uL/min Flow Rate | 1,500:1 RMS (1 pg LC/MS injection of reserpine. Signal-to-noise for most intense product ions (174, 195, 397, and 448 m/z) while maintaining a resolution of 40,000 at m/z 2,722 in 4 GHZ mode) |
| Mass Resolving Power | 45,000 FWHM at m/z 2,722 (measured after automatic tuning procedure) |
| Mass Accuracy - MS Mode, Electrospray On-Column, 400 uL/min | Less than 0.8 ppm RMS as measured from 10 repeat injections (measured at the (M+H)+ ion of reserpine (m/z 609.2807) using an internal mass reference) |
| Mass Accuracy - MS/MS Mode, Electrospray On-Column, 400 uL/min | Less than 2 ppm RMS on m/z 397 as measured from 10 repeat injections (product ion m/z 397 for reserpine) |
| Dynamic Range | Up to 5 decades (intrascan dynamic range on co-eluting components) |
| Mass Range | m/z 100 to 10,000 for extended mass range m/z 50 to 1,700 or 50 to 3,200 for both high resolution and extended dynamic range modes Quadrupole up to m/z 4,000 |
| Spectral Acquisition Rate, MS Mode | 50 spectra/second (m/z 50 to 1,700 in MS mode while maintaining a resolution of 40,000 at m/z 2,722 in 4 GHZ mode) |
| Spectral Acquisition Rate, MS/MS Mode | 30 MS/MS spectra/second (m/z 50 to 1,700 in MS/MS mode while maintaining a resolution of 40,000 at m/z 2,722 in 4 GHZ mode) |
| Positive to Negative Switching | 1.5 seconds (complete cycle switching from positive to negative and positive modes allow for stabilization time) |
| Dimensions (H x D x W) | 76 x 30 x 48 in. (193 x 76.2 x 121.9 cm) |
| Weight | 375 lb. (170 kg) |
| Electrical Requirements | 200-240VAC, 50/60Hz |
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