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Higgins PROTO300 C18 Semi-Prep Reversed-Phase HPLC Column 25 cm × 20 mm × 10µm
Product Description
The Higgins Analytical PROTO300 C18 Semi-Prep LC Column is engineered expressly for reversed-phase high-performance liquid chromatography (RP-HPLC) applications involving purification of large biomolecules such as proteins and peptides. Specifically designed for bioprocessing workflows, it provides enhanced resolution and high capacity needed in molecular diagnostics and biopharmaceutical production pipelines.
Key technical specifications include:
- Column dimensions: 25 cm length × 20 mm internal diameter × 10 µm particle size
- Pore size: 300Å, optimal for large biomolecule separation including cyclized peptides
- Surface area: 100 m²/g, contributing to high binding capacity
- Pore volume: 0.9 mL/g, facilitating efficient mass transfer
- Phase type: Monofunctional & fully endcapped C18 phase enhancing peak shape and reproducibility
- Resilient under extreme operating pH conditions ranging from 1.5 to 10, accommodating typical bioprocessing column washing protocols without compromising column longevity
This semi-preparative LC column is widely adopted in synthetic biology research and molecular cloning workflows requiring reliable and robust chromatographic separation of peptides and proteins. Its design supports live-cell imaging sample preparation through precise protein purification and is critical in biomanufacturing quality control operations.
Ideal for researchers and laboratory professionals engaged in cell imaging, biotechnology, and clinical diagnostics, the Higgins PROTO300 delivers superior performance for preparative and semi-preparative purification tasks in cutting-edge life sciences research.
Item Stats
Product details
| Manufacturer | Higgins Analytical Inc |
| Model | PROTO300 C18 |
| Year | 2017 |
| Condition | Used |
| Serial Number | 249298, 249367, 249376 and 271545 |
| MPN | RS-2520-W181 |
| Condition Description | IN GOOD CONDITION |
| Dimensions | 12"L x 2"W x 2"H |
| Weight (lbs) | 1 |
| Country/region of manufacture | United States |
| Custom Label | G4 |
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