Offering the best quotes for your research!
New and used scientific instruments
🚚 Shipping Worldwide 🌎
✉️ info@questpair.com
GE AKTA Pure 25 L Fast Protein Liquid Chromatography System
Product Description
The GE AKTA Pure 25 L FPLC System is designed for flexible and precise purification of proteins, peptides, and nucleic acids with the following features:
- Flow rate range: 0.001 to 25 ml/min (extendable to 50 ml/min for column packing)
- Maximum operating pressure: 20 MPa (2900 psi)
- UV monitor: Fixed wavelength at 280 nm, absorbance range −6 to 6 AU with high linearity and low noise
- Conductivity monitor: 0.01 to 999.9 mS/cm range
- pH monitor: Accurate readings from 0 to 14 pH units over calibrated temperature
- Fraction collector (F9-C): Capacity up to 576 fractions, compatible with multiple vessel types including deep well plates, fraction volumes 0.1 to 250 ml
- Software: UNICORN, enabling seamless hardware, column, and resin integration
- Modular design: Extensive customization options for varied purification tasks and scales
- Precision: Flow rate RSD less than 0.5%, ensuring reproducibility
- Designed for multiuser environments and large-scale preparative applications
- Backed by over 50 years of protein research experience and 30 years in chromatography system development
This system supports microgram to gram scale target production with robust monitoring and fraction collection to optimize protein purification workflows.
Item Stats
Product details
| Flow Rate Range | 0.001 to 25 ml/min (up to 50 ml/min during column packing) |
| Flow Rate Accuracy | ± 1.2% |
| Flow Rate Precision | RSD <0.5% |
| Pressure Range | 0 to 20 MPa (2900 psi) |
| Pressure Limits | Programmable upper and lower |
| Viscosity Range | 0.35 to 10 cP (5 cP above 12.5 ml.min) |
| Wavelength Range | 280 nm (Fixed) |
| Absorbance Range | -6 to 6 AU |
| Resolution | 0.001 mAU |
| Wavelength Reproducibility | ± 0.01 nm |
| Linearity | Within ± 5% at 0 to 2 AU |
| Drift | ≤ | 0.2 mAU | AU/h (2 mm cell at 280 nm) |
| Noise | < 0.1 mAU |
| Operating Pressure | 0 to 2 MPa |
| Lamp Operating Time | > 10,000 hours |
| Flow Cell | Optical path length: 2 mm Cell volume: 2 ul Total volume: 30 ul |
| Conductivity Range | 0.01 to 999.9 mS/cm |
| Accuracy | ± 0.01 mS/cm or ± 2%, whichever is greater (within 0.3 to 300 mS/cm) |
| Operating Pressure | 0 to 5 MPa |
| Flow Cell Volume | 22 ul |
| Temperature Monitor Range | 0°C to 99°C |
| Temperature Monitor Accuracy | ± 1.5°C within 4°C to 45°C |
| pH Reading Range | 0 to 14 |
| Accuracy | ± 0.1 pH unit within pH 2 to 12, temperature within ± 3°C from calibration temperature |
| Operating Pressure | 0 to 0.5 MPa |
| Flow Cell Volume | 76 ul |
| Number of Fractions | Up to 576 |
| Vessel Types | Deep well plates, 96, 48, or 24 wells |
| Fraction Volumes | 0.1 to 250 ml |
| Spillage-Free Mode | Automatic, Drop Sync, or Accumulator |
| Flammable Liquids | No |
| Delay Volume (UV-Dispenser Head) | 205 ul, 86 ul with optional tubing kit (i.d. 0.25 mm) |
| Dimensions (W x D x H) | 15.4 x 23 x 12.6 (39 x 58.5 x 32 cm) |
| Weight | 46.3 lb. (21 kg) |
Documents
About QuestPair
Our Mission
QuestPair is Europe's leading platform for scientific equipment, connecting researchers and organizations with verified suppliers of both new and pre-owned laboratory instruments in the biotech and material science industry. Our aim is to make the procurement of scientific instruments more efficient, cost-effective, and reliable, so that laboratories can focus on advancing science rather than searching equipment and negotiating deals.
Why Choose Us
Founded by scientists for scientists, we understand your challenges. Our AI-powered platform offers transparent pricing, verified quality, and expert support, ensuring you find the perfect equipment for your research needs.
Verified Quality
Every piece of equipment undergoes thorough verification by our expert team, ensuring reliability and performance.
Cost Efficiency
Access both new and premium pre-owned equipment, saving up to 40% without compromising on quality.
Expert Support
Our dedicated team provides personalized guidance throughout your equipment procurement journey.
Ready to Transform Your Research?
Join thousands of biotech scientists who trust QuestPair for their equipment needs.
What customers think about QuestPair
Brilliant service. Everything arrived and was exactly as expected
Jon Welburn
Great customer service! Got me great options for the equipment I was looking for and in a timely manner.
Pamela
Space Lab Technologies, LLCHarm is very responsive to help me find the right equipment. The product I received is in a good condition.
Ph.D. Hsin-Wen Liang
Northeastern UniversityFrom €46,375.78*ex. VAT
In Stock
Get a quote within one business day
Verified equipment
Trusted warranties & returns
Safe payment and fast delivery
Didn't find what you're looking for?
Our sales team specializes in sourcing custom and hard-to-find products to meet your exact specifications. Submit a request for a tailored quote.
Frequently Asked Questions
Is this equipment new or refurbished?
How long does shipping take?
What about warranty and returns?
Why request a quote?
Need help choosing the right tool?
- Accurate policy information specific to your chosen product
- Clear understanding of warranty coverage and terms
- Detailed return policy explanation
- Transparent shipping options and costs
Customer Service
Here to help you with: Product Inquiries, Shipping & Support, Technical Support, Business Inquiries and Press.
We are available to assist you Mon-Fri, 10am - 5pm CET.