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GE AKTA Pure 150 L Fast Protein Liquid Chromatography System for Protein and Nucleic Acid Purification
Product Description
The GE AKTA Pure 150 L is a modular Fast Protein Liquid Chromatography (FPLC) system designed for precise purification of proteins, peptides, and nucleic acids. It features a robust flow rate range of 0.01 to 150 ml/min (up to 50 ml/min for column packing) with high flow precision (RSD < 0.5%) and operates under pressures up to 5 MPa (725 psi), supporting both microgram and gram-scale preparations.
Key technical characteristics include:
- UV Monitor: Fixed wavelength at 280 nm ideal for detecting aromatic protein residues, with an absorbance range of -6 to 6 AU ensuring sensitive biomolecule detection essential for molecular diagnostics and bioprocessing.
- Conductivity Monitor: Measures from 0.01 to 999.9 mS/cm, enabling precise buffer and salt gradient management necessary for synthetic biology and biomanufacturing workflows.
- pH Monitor: Accurate measurement across 0 to 14 pH units, supporting stable chromatographic separations critical in gene editing and biopharmaceutical production pipelines.
- F9-C Fraction Collector: Supports up to 576 fractions with fraction volumes ranging from 0.1 to 250 ml, compatible with diverse vessel types including deep well plates, facilitating high-throughput workflows in molecular cloning and protein engineering research.
- UNICORN Software Integration: Provides seamless control and monitoring of hardware, columns, and resins, ensuring reproducibility and streamlining complex purification protocols for biotechnology research applications.
Widely adopted among molecular biology researchers, this FPLC system is suited for applications in biomedical engineering, cell imaging, and molecular diagnostics, offering extensive customization for tailoring purification strategies. With over 50 years of protein research expertise from GE Healthcare, the AKTA Pure 150 L remains a critical instrument in advanced bioprocessing and synthetic biology research, delivering reliable and high-purity separations essential in contemporary laboratories.
Item Stats
Product details
| Flow Rate Range | 0.01 to 150 ml/min (up to 300 ml/min during column packing) |
| Flow Rate Accuracy | ± 1.5% |
| Flow Rate Precision | RSD <0.5% |
| Pressure Range | 0 to 5 MPa (725 psi) |
| Pressure Limits | Programmable upper and lower |
| Viscosity Range | 0.35 to 5 cP |
| Wavelength Range | 280 nm (Fixed) |
| Absorbance Range | -6 to 6 AU |
| Resolution | 0.001 mAU |
| Wavelength Reproducibility | ± 0.01 nm |
| Linearity | Within ± 5% at 0 to 2 AU |
| Drift | ≤ | 0.2 mAU | AU/h (2 mm cell at 280 nm) |
| Noise | < 0.1 mAU |
| Operating Pressure | 0 to 2 MPa |
| Lamp Operating Time | > 10,000 hours |
| Flow Cell | Optical path length: 2 mm Cell volume: 2 ul Total volume: 30 ul |
| Conductivity Range | 0.01 to 999.9 mS/cm |
| Accuracy | ± 0.01 mS/cm or ± 2%, whichever is greater (within 0.3 to 300 mS/cm) |
| Operating Pressure | 0 to 5 MPa |
| Flow Cell Volume | 22 ul |
| Temperature Monitor Range | 0°C to 99°C |
| Temperature Monitor Accuracy | ± 1.5°C within 4°C to 45°C |
| pH Reading Range | 0 to 14 |
| Accuracy | ± 0.1 pH unit within pH 2 to 12, temperature within ± 3°C from calibration temperature |
| Operating Pressure | 0 to 0.5 MPa |
| Flow Cell Volume | 76 ul |
| Number of Fractions | Up to 576 |
| Vessel Types | Deep well plates, 96, 48, or 24 wells |
| Fraction Volumes | 0.1 to 250 ml |
| Spillage-Free Mode | Automatic, Drop Sync, or Accumulator |
| Flammable Liquids | No |
| Delay Volume (UV-Dispenser Head) | 205 ul, 86 ul with optional tubing kit (i.d. 0.25 mm) |
| Dimensions (W x D x H) | 15.4 x 23 x 12.6 (39 x 58.5 x 32 cm) |
| Weight | 46.3 lb. (21 kg) |
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