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GE ÄKTA FPLC Explorer 100 System for Protein Purification
Product Description
The GE ÄKTA FPLC Explorer 100 System is designed for precise protein purification using:
- Wavelength Range: 190–700 nm with 1 nm step increments
- Wavelength Accuracy: ±2 nm
- Max Flow Rate: 100 ml/min
- UV-900 Detector: Simultaneous detection at three wavelengths, 4 nm bandwidth, flow cell max pressure 2 MPa
- P-900 Pump: Accurate flow rates from 0.01 to 100 ml/min, supports isocratic and gradient modes
- M-925 Mixer: Six interchangeable chambers, flow rate range 0.001 to 100 ml/min, max pressure up to 35 MPa
- Frac-950 Fraction Collector: Wide range of fraction sizes, pH stability 1 to 13
- System supports size exclusion, ion exchange, and affinity chromatography applications
- Compatible with Unicorn 5.31 software for operation and data handling
- Tested for full functionality including pressure sensors, valves, UV monitor, pH and conductivity measurements
This system is suitable for proteomics, drug discovery, biochemistry, molecular biology, and other protein research fields.
Item Stats
Product details
| Wavelength range | 190–700 nm in steps of 1 nm, 3 wavelengths simultaneously |
| Bandwidth | 4 nm |
| Wavelength accuracy | ±2 nm |
| Wavelength reproducibility | < 500 ms (one cycle from 214 nm to 254 nm and back to 214 nm) |
| Linearity | < 2% deviation up to 2 AU at 260 nm with Uracil at pH 2 |
| Static noise | short term: < 6x10-5 AU at 230 nm long term: < 6x10-5 AU at 230 nm |
| Drift | < 2x10-4 AU/h at 254 nm |
| Optical path length 2 mm cell | 2 mm, 2 µl cell volume |
| Optical path length 10 mm cell | 10 mm, 8 µl cell volume |
| Max. flow rate | 100 ml/min |
| Max. pressure | 2 MPa (290 psi) |
| Temperature range | +4 to +40 °C (liquid) |
| Wetted material | PTFE (polytetrafluoroethylene) PEEK (polyetheretherketone) titanium (palladium alloy), quartz (synthetic fused silica) and titanium |
| Flow Rate Range | Isocratic mode: 0.01 to 100 ml/min in steps of 10 ul/min Gradient mode: 0.01 to 100 ml/min in steps of 10 ul/min Double mode: 0.01 to 200 ml/min in steps of 10 ul/min |
| Pressure Range | 0 to 10 MPa (100 bar, 1450 psi) |
| pH Stability Range | 1 to 13 (1 to 14 with <1 day exposure) |
| Viscosity | Max 5 cP |
| Flow Rate Accuracy | ± 2% or 20 ul/min, whichever is greater |
| Flow Rate Reproducibility | Flow rate ≥ 0.5 ml/min: rsd < 0.5% |
| Gradient Composition | Accuracy: < ±1% at 0.05 to 10 ml/min Reproducibility: rsd < 0.25% at 0.5 to 100 ml/min |
| Pressure Sensor | Range: 0 to 27.5 MPa Offset error: < 0.05 MPa Scale error: < ±2% |
| Max Flow Rate | 0.001 to 100 ml/min |
| Max Pressure | 12ml chamber: 10 MPa at 0.1 to 100 ml/min (100 bar, 1450 psi) 0.6, 2, and 5ml chambers: 25 MPa at 0.001 to 100 ml/min (250 bar, 3625 psi) 90 ul and 0.2 ml chambers: 35 MPa at 0.001 to 5 ml/min (250 bar, 5075 psi) |
| pH Stability Range | 1 to 13 (1 to 14 with <1 day exposure) |
| Viscosity | Max 5 cP |
| Internal Volume | 6 interchangeable mixing chambers: 90 ul, 0.2, 0.6, 2, 5, and 12 ml |
| Mixing Principle | 1 static chamber and 1 dynamic chamber |
| Inlet and Outlet Tubing | UNF 10 to 32 thread profile |
| Max Flow Rate | 100 ml/min |
| pH Stability Range | 1 to 13 (1 to 14 with <1 day exposure) |
| Fraction Size | 0.1 to 99999.99 ml (volume mode) 0.1 to 99999.99 min (time mode) |
| Standard Mode | u-liter plates, DropSync: 0 to 1 ml/min 12 mm rack, DropSync: 0 to 1.5 ml/min 18 and 30 mm racks, DropSync: 0 to 2 ml/min 18 and 30 mm racks, Accumulator: 15 to 100ml/min |
| Prep Mode | 30 mm rack, Accumulator: 15 to 100 ml/min Funnel-to-flask, Accumulator: 15 to 100 ml/min250ml bottles, Accumulator: 15 to 100 ml/min |
| Environmental Conditions | +4° to +40°C 20 to 95% relative humidity84 to 106 kPa (840 to 1060 mbar) atmospheric pressure |
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