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Amersham Biosciences AKTA Purifier 10 FPLC System with UV-900 Detector for Protein Purification
Product Description
The Amersham Biosciences AKTA Purifier 10 FPLC System with UV-900 Detector is a modular, versatile fast protein liquid chromatography platform designed for efficient purification of proteins, peptides, and nucleic acids. It supports purification scales from microgram to milligram quantities, operating across a flow rate range of 0.001 to 10 mL/min and pressure limits up to 25 MPa, accommodating a wide range of chromatography protocols used in molecular biology, biotechnology, and molecular diagnostics workflows.
Key technical features include:
- P-900 Pump with tested precise flow rate accuracy for reproducible gradient elution.
- UV-900 Detector providing reliable UV absorbance monitoring essential for protein and peptide quantitation.
- PHC-900 Monitor and pH/C-900 modules, enabling continuous pH and conductivity measurements critical in buffer optimization and bioprocessing applications.
- Tested valve motors ensuring robust fractionation control with the Frac-920 fraction collector via manual or software-driven commands.
- Full system compatibility with the Unicorn 5.31 operating system, facilitating automated method development and data acquisition.
The system is widely adopted in synthetic biology research and biopharmaceutical production pipelines for tasks such as protein purification, molecular cloning workflows, and biomanufacturing process development. Note: The pH probe and holder are not included and can be acquired separately based on specific assay requirements.
This AKTA Purifier 10 setup is popular among researchers focusing on biotechnology, synthetic biology, and clinical diagnostics who require high accuracy and flexible system integration in liquid chromatography-based workflows.
Item Stats
Product details
| Flow Rate Range | Isocratic mode: 0.001 to 10 ml/min in steps of 1 ul/min Gradient mode: 0.001 to 10 ml/min in steps of 1 ul/min Double mode: 0.001 to 20 ml/min in steps of 1 ul/min |
| Pressure Range | 0 to 25 MPa (100 bar, 1450 psi) |
| pH Stability Range | 1 to 13 (1 to 14 with <1 day exposure) |
| Viscosity | Max 5 cP |
| Flow Rate Accuracy | ± 2% or 20 ul/min, whichever is greater |
| Flow Rate Reproducibility | Flow rate ≥ 0.5 ml/min: rsd < 0.5% |
| Gradient Composition | Accuracy: < ±1% at 0.05 to 10 ml/min Reproducibility: rsd < 0.25% at 0.5 to 100 ml/min |
| Pressure Sensor | Range: 0 to 27.5 MPa Offset error: < 0.05 MPa Scale error: < ±2% |
| Total Volume Between Inlet and Outlet | < 800 ul/module |
| Wavelength range | 190–700 nm in steps of 1 nm, 3 wavelengths simultaneously |
| Bandwidth | 4 nm |
| Wavelength accuracy | ±2 nm |
| Wavelength reproducibility | < 500 ms (one cycle from 214 nm to 254 nm and back to 214 nm) |
| Linearity | < 2% deviation up to 2 AU at 260 nm with Uracil at pH 2 |
| Static noise | short term: < 6x10-5 AU at 230 nm long term: < 6x10-5 AU at 230 nm |
| Drift | < 2x10-4 AU/h at 254 nm |
| Max Flow Rate | 0.001 to 10 ml/min |
| Max Pressure | 12ml chamber: 10 MPa at 0.1 to 100 ml/min (100 bar, 1450 psi) 0.6, 2, and 5ml chambers: 25 MPa at 0.001 to 100 ml/min (250 bar, 3625 psi) 90 ul and 0.2 ml chambers: 35 MPa at 0.001 to 5 ml/min (250 bar, 5075 psi) |
| pH Stability Range | 1 to 13 (1 to 14 with <1 day exposure) |
| Viscosity | Max 5 cP |
| Internal Volume | 6 interchangeable mixing chambers: 90 ul, 0.2, 0.6, 2, 5, and 12 ml |
| Mixing Principle | 1 static chamber and 1 dynamic chamber |
| Inlet and Outlet Tubing | UNF 10 to 32 thread profile |
| Capacity | 95 fractions in 10-18 mm tubes |
| Depth | 400 mm |
| Flow Rate | < 100 ml/min |
| Height | 250 mm |
| Operating Temperature | 4° to 40°C |
| pH Stability Long Term | 1 – 13 |
| pH Stability Short Term | 1 – 14 |
| Program Memory | 99 programs |
| Rack Designation | 95 x 10-18 mm (Standard) 175 x 12 mm and 40 x 30 mm (Optional) |
| Relative Humidity | 20 to 95% (non condensing) |
| Time Mode | 0.01 to 9.999 min |
| Volume Mode | 0.1 to 999 ml |
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