Applied Biosystems StepOne Plus PCR System

The StepOnePlus Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. The StepOnePlus Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 4-color optical recording, the StepOnePlus Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.

Advanced software and instrumentation for performing a wide array of genomic assays

Sensitive 4-color optical LED recording system

Intuitive and robust software perfect for both first-time and advanced users

Simple and flexible instrument set-up and usage New StepOne Software

The StepOne Software included with the StepOnePlus Real-Time PCR System runs on the Windows XP operating system and provides instrument control, data collection, and data analysis capabilities (analysis software is also compatible with Windows 7).

This latest version includes capabilities to collect melt curve data for High Resolution Melt (HRM) applications and the option to export in Real-Time PCR Data Markup Language (RDML) for compatibility with MIQE guidelines.

Rapid and Simple Assay Set-Up This remarkably simple Real-Time PCR system is designed with an LCD touchscreen and a user-friendly interface that brings the power of genetic studies to researchers new to Real-Time PCR.

The intuitive software and protocol wizards help guide new users through their Real-Time PCR experiments.

The StepOnePlus Real-Time PCR System also includes a quick-start setup so that you start a run immediately and enter plate information at a later time.

Sensitive LED-Based 4 Color Fluorescence Reading

The StepOnePlus Real-Time PCR System utilizes a long-life LED-based optical system that can record fluorescence from FAM/SYBR Green, VIC/JOE, NED/TAMRA, and ROX dyes.

This cost-effective, 4-color, 96-well system delivers precise, quantitative Real-Time PCR results and saves data from all filters in every run without depending on a computer or plate setup.

It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan assay with 99.7% confidence. Compatible with Many Genomic Analysis Techniques

Perform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOnePlus Real-Time PCR System and StepOne Software.

The StepOnePlus System supports many Real-Time PCR applications, including the following:

SNP Genotyping

Gene Expression Analysis

MicroRNA Expression

Protein Expression

Translocation Analysis

Gene Detection

Viral Load Analysis

The included StepOne Software supports a variety of analysis methods , including the following:

Standard Curve (absolute quantitation)

Relative Standard Curve

Comparative Ct (ΔΔCt) (relative quantitation)

Genotyping and Presence/Absence

Melt Curve Analysis (as a standalone application)

High Resolution Melting (as a standalone applications)