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Applied Biosystems 7500 Fast Real-Time PCR System with 96-Well Block and Fast Cycling
Product Description
The Applied Biosystems 7500 Fast Real-Time PCR System is designed for rapid, precise nucleic acid quantification and detection featuring:
- Block Configuration: 96-well fast block optimized for 10 µL reaction volumes (range 5–30 µL)
- Maximum Ramp Rate: 5.5°C per second for accelerated thermal cycling
- Temperature Uniformity: ±0.5°C, with accuracy of ±0.25°C ensuring consistent thermal control
- Optical System: CCD camera with halogen lamp and five excitation/emission filters supporting multiplex detection of up to five targets per well
- Dynamic Range: Nine logarithmic orders enabling detection from single-copy templates with 99.7% confidence in 20 µL reactions
- Applications: Suitable for gene expression analysis, DNA quantification, and other nucleic acid assays requiring fast and accurate results
- Performance: Delivers results in under 30 minutes without compromising assay quality or extension times
Additional Note: Dye calibration is recommended for application-specific dyes as per the Operator's Manual. The unit is fully tested to meet factory specifications before shipment.
Item Stats
Product details
| Block Configuration | 96-Well Fast (5 to 30 uL reactions, optimized for 10 uL reactions) |
| Temperature Range | 4°C to 100°C |
| Temperature Uniformity | ± 0.5°C (30 seconds after clock start) |
| Temperature Accuracy | ± 0.25°C of setpoint/display temperature (measured at 3 minutes after clock start) |
| Maximum Ramp Rate | 5.5°C/second |
| Run Time | < 30 minutes |
| Optical System | CCD camera with halogen lamp excitation; five-exciation and five-emission filters |
| Dynamic Range | 9 logs of linear dynamic range |
| Sensitivity | Detection of 1 copy of template in 20 uL reaction for a single reporter TaqMan assay, with 99.7% confidence |
| Multiplexing | Up to 5 targets per well |
| Dye Compatibility | FAM/SYBR Green, VIC/JOE, NED/TAMRA/Cy3, ROX/Texas Red, and Cy5 (Calibration for new dyes within the wavelength range is possible by following the custom dye calibration procedure in the user’s manual) |
| Passive Reference Dyes | ROX dye or any calibrated dye |
| Dimensions (W x D x H) | 13.9 x 17.7 x 19.3 in. (34 x 45 x 49 cm) |
| Weight | 75 lb. (34 kg) |
| Electrical Requirements | 100-240V, 50/60Hz, 1080W |
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Northeastern UniversityFrom €7,725.72*ex. VAT
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