Roche LightCycler 96 Real-Time PCR System Model 5815916001

The Roche LightCycler 96 real-time PCR system (Model 5815916001) is a high-precision molecular diagnostics instrument designed for rapid and sensitive quantitative PCR applications. Its advanced Peltier-based temperature control ensures accuracy within ±0.2°C and uniformity of ±0.3°C across samples, supporting temperature ranges from 37 to 98°C and gradient functionality up to 20°C, ideal for optimizing bioprocessing assays and gene expression analysis workflows.

Featuring a broad-spectrum high-power LED excitation system with four fixed wavelength pairs (470/514 nm, 533/572 nm, 577/620 nm, 645/697 nm), this instrument supports multiplex fluorescence detection for dyes such as SYBR Green I, FAM, VIC, HEX, Texas Red, and Cy5. The 96x2 glass fiber optic detection with CCD camera enhances sensitivity and reproducibility, critical for applications in molecular diagnostics and synthetic biology research.

Run times are notably rapid with 40-cycle protocols completing within 40 minutes, facilitating efficient throughput in molecular cloning, gene editing validation, and biomanufacturing quality control. The system supports flexible reaction formats including 96-well plates, 8-strip tubes, and 0.1 mL blocks, accommodating reaction volumes from 10 to 50 µL per well.

Data analysis features integrated software capable of absolute and relative quantification, melt curve (Tm) analysis, high-resolution melting (HRM), and genotyping endpoint calls. Operation is intuitive with a touchscreen interface that requires no PC, though data transfer via USB and LAN connectivity is supported. The LightCycler 96 is compatible with Windows XP through Windows 11 OS platforms.

Physically robust at approximately 27 kg and measuring 40 cm (W) x 40 cm (D) x 53 cm (H), it is suitable for benchtop installation in clinical diagnostics labs, biotechnology facilities, and research environments focusing on live-cell imaging and molecular diagnostics. This used unit is provided as-is, with original manuals included, and may exhibit performance variations compared to new condition equipment.