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Marketplace
Analytics
Brand
Pharmacia Biotech
Supplier
LabMakelaar Benelux B.V.
Location
Zevenhuizen - Netherlands
Price
€399.00 
(ex. VAT)
This item is
for sale
State of item
used
Categories

Pharmacia Biotech EPS 600 Electrophoresis Power Supply

LabMakelaar Benelux B.V.€399.00 (ex VAT)
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Description by the supplier

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System consists of: Bio-Rad Mini-Sub Cell GT Electrophoresis Cell Bio-Rad PowerPac HV High-Voltage P (more)ower Supply Mini-Sub Cell GT: Bio-Rad offers a range of mini horizontal electrophoresis chambers to resolve DNA fragments on either handcast agarose gels or ReadyAgarose precast gels. These cells include a buffer tank, safety lid with cables, and leveling bubble. Fast and flexible, the Mini-Sub cell GT cell is our most popular classroom gel box. The Mini-Sub cell GT cell holds either a 7 x 7 cm or a 7 x 10 cm gel tray. A 10 cm agarose gel poured with two 8-well combs easily accommodates two student teams at a time. At 100 V, this cell resolves DNA fragments from 20–20,000 base pairs in just under 30 min. Cell size (WxLxH): 120 x 260 x 65 mm Gel tray sizes (OD) (WxL): 70 x 70 mm ReadyAgarose gels accommodated: Yes, mini format Sample throughput: 8–30 Base buffer volume: ~270 ml Buffer recirculation: No Bromophenol blue migration: 4.5 cm/hr (at 75 V) PowerPac HV: Output (programmable): Voltage 20–5,000 V, Current 0.01–500 mA, Power 1–400 W Type of output: Constant voltage, constant current, constant power, or constant temperature (with automatic crossover) Timer control: 1 min–99 hr 59 min, fully adjustable Volt-hour control: Yes, 99,999 V-hr Pause/resume function: Yes Display functions: 128 x 64 pixel, yellow-green backlit graphics LCD Programmable methods: Stores up to 9 basic and 9 IEF methods, each with up to 9 steps Real-time editing: Yes Real-time clock: Yes Automatic recovery after power failure: Yes, user-selectable; setup values maintained Data transfer/archiving: Yes Temperature control: Yes, via temperature probe (not included); 30–90°C ±2°C Microampere readout and control: Yes Safety features: No-load detection, sudden load change detection, ground leak detection, overload/short circuit protection, overvoltage protection Number of output jacks: 4 sets in parallel Dimensions (WxLxH): 75 x 340 x 100 mm Weight: 2.85 kg

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This system is used to analyze the size of DNA/RNA samples, with convenientanalytical operability. I (more)t achieves analysis costs on par with agarose gel electrophoresis, and can perform fully automatic analyses of up to 108 samples. Using optimized reagent kits (four types for DNA analysis and one type for RNA analysis), the system achieves a high resolution and high sensitivity. It can significantly improve the workflow for mutation checks in genome editing, and genotype determination. Sample rack: Compatible with 96-well PCR plate and 12/8-strip PCR tube (Shimadzu recommended product) Microchip: Quartz, 23 mm separation channel length, on-chip electrodes (insert up to four microchips) Pretreatment: Automatic sample injection, automatic separation buffer replenishing, automatic chip cleaning Electrophoresis Voltage: Max. rated voltage: 1.5 kV, max. current: 250 μA Analysis Cycle time: Approx. 80 s (using four chips) DNA standard analysis (DNA-1000/premixed). This does not include time required for carrying out the first and final wash and initial analysis. Detection Method: LED-excited fluorescence detector (470 nm excitation wavelength) Separation Size Range: 25 to 500 bp (DNA-500 Kit), 100 to 1000 bp (DNA-1000 Kit), 100 to 2500 bp (DNA-2500 Kit), 100 to 12000 bp (DNA-12000 Kit), Up to 28S rRNA (5.0 knt) (RNA Kit) Resolution: 5 bp (25 to100 bp), 5% (100 to 500 bp), 10% (500 to 1000 bp), 20% (1000 to 12000 bp) Sizing Accuracy: ±5 bp (25 to 100 bp), ±5% (100 to 500 bp), ±15% (DNA-1000, DNA-2500, DNA-12000) Required Sample Volume: DNA analysis: Premix mode: 2 to 10 μL (after mixing with marker solution: 6 to 30 μL), On-Chip Mixing mode: 5 to 30 μL, RNA analysis: Premix mode: 3 to 15 μL (after mixing with marker solution: 6 to 30 μL), In the Premix mode, the marker solution is mixed with the sample before loading in the instrument, In the On-Chip Mixing mode, the sample and marker solution are loaded separately and mixed on the microchip under program control. Maximum Salt Concentration: DNA analysis: 10mM Tris-HCI containing 125 mM KCl or NaCl max, RNA analysis: 10mM Tris-HCI, containing 1 mM EDTA max. Min. Detection Limit: DNA analysis: 0.2 ng/μL (at 10mM Tris-HCI buffer, containing 50 mM KCl and 1.5 mM MgCl2), RNA analysis: 5 ng/μL (total RNA), 25 ng/μL (mRNA) (at 10 mM Tris-HCI buffer, containing 1mM EDTA) Quantitation Range: DNA analysis: 0.5 to 50 ng/μL (at 10mM Tris-HCI, containing 50 mM KCl and 1.5 mM MgCl2), RNA analysis: 25 to 500 ng/μL (total RNA), 25 to 250 ng/μL (mRNA) (10 mM Tris-HCI buffer, containing 1 mM EDTA) Quantitation Accuracy: DNA analysis: ±30% (at 10 mM Tris-HCI buffer, containing 50 mM KCL) (DNA-500, DNA-1000, and DNA-2500 Kits), ±40 % (DNA-12000 kit. Quantitative accuracy is based on verification from 200 bp to 12000 bp.) Quantitation Repeatability: RNA analysis: CV 10% or less (CV 20% or less for eukaryotic-origin total RNA at concentrations of 150 ng/μL or more) Dimensions (WxDxH): 415 x 545 x 508 mm Weight: 43 kg

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