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Thermo Fisher Attune NxT Acoustic Focusing Flow Cytometer with Cytkick Autosampler V4B3Y4R3
Product Description
The Thermo Fisher Attune NxT Acoustic Focusing Cytometer V4B3Y4R3 is a state-of-the-art benchtop flow cytometer designed for advanced multiparametric cell analysis, essential in molecular biology, immunology, oncology, and biotechnology research applications such as gene expression profiling and synthetic biology.
- Optical Configuration: Equipped with 4 excitation lasers (405 nm violet, 488 nm blue, 561 nm yellow, and 637 nm red) delivering detection across 14 fluorescence channels utilizing 16 detectors for enhanced multicolor assays in complex cellular environments.
- Acoustic Focusing Technology: Utilizes ultrasonic waves to precisely align cells in a single stream, improving sensitivity, reducing clogging, and enabling robust data acquisition from heterogeneous samples.
- Sample Handling & Throughput: Integrated Cytkick autosampler supports high-throughput workflows compatible with 96-well, 384-well, and deep-well plate formats, facilitating unattended processing (<42 min for 96-well, <180 min for 384-well). Flow rate ranges from 12.5 to 1000 µL/min with sample volumes from 20 µL to 4 mL.
- Performance Specs: Capable of acquiring up to 35,000 events/second with 34 parameters, maximum electronic speed of 65,000 events/second. Particle detection ranges from 0.5 µm to 50 µm, suitable for a broad spectrum of cellular and microparticle analyses.
- No-Wash, No-Lyse Protocols: Supports gentle sample preparation workflows to maintain cell integrity, critical for molecular diagnostics and live-cell immunophenotyping.
- Software Integration: Included is a 1-year subscription to De Novo FCS Express 7 Analysis software (RUO), offering advanced data import, gating, statistical analysis, high-quality visualization, automated reporting, and compatibility with multiple flow cytometry data file formats. This software is widely adopted in bioprocessing and biopharmaceutical research pipelines.
- System Customization: Optional fluidics extensions (EFS1) and alternative filter sets available, enabling customization for specific fluorophore panels or assay requirements.
- Validation and Quality Assurance: The instrument undergoes thorough testing including fluidics decontamination, laser alignment with mW-level verification, detector calibration with standardized beads, electronic noise assessment, and performance tracking ensuring reproducible coefficient of variation (CV) values vital for rigorous research reproducibility.
- Applications: Ideal for immunophenotyping in vaccine development, rare cell detection in oncology, multiplexed protein expression in drug discovery, and cell sorting in molecular biology workflows. Its high sensitivity and multi-laser design make it indispensable for advanced synthetic biology and molecular cloning studies.
- Warranty & Support: Comes with 90 days continental US warranty (45 days international), with optional extended warranty and remote or onsite installation/training available especially near Boston, MA. Full after-sales technical support ensures reliable integration into research programs.
This instrument’s acoustic cell alignment combined with a high-parameter laser and detector array, plus automated sample handling, makes it a crucial tool for biotechnology researchers emphasizing bioprocessing, molecular diagnostics, and biomanufacturing in clinical and translational research environments.
Item Stats
Product details
| Stock | In Stock |
| Brand | Thermo Fisher Scientific |
| Product Code | SM100886-V4B3Y4R3-Cytkick |
| Condition | Pre-Owned |
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